FastQCFastQC Report
Thu 2 Feb 2017
SRR4064154_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064154_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936454
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT241991.2496552977762447No Hit
GTCCTACAGTGGACATTTCTAAATT205211.0597204994283365No Hit
CTTTAGGACGTGAAATATGGCGAGG204791.0575515865597633No Hit
CTGTAGGACGTGGAATATGGCAAGA197161.0181496694473506No Hit
GTCCTACAGTGTGCATTTCTCATTT143430.7406837446177394No Hit
CTGTAGGACCTGGAATATGGCGAGA108200.5587532675705181No Hit
ATTTAGAAATGTCCACTGTAGGACG105460.5446036931422074No Hit
CTGAAGGACCTGGAATATGGCGAGA102860.5311770896700877No Hit
GTCCTTCAGTGTGCATTTCTCATTT93730.4840290551699137No Hit
TTTCTAAATTTTCCACCTTTTTCAG82210.42453887363190657No Hit
GATATACACTGTTCTACAAATCCCG69470.35874851661852025No Hit
GAATATGGCAAGAAAACTGAAAATC66760.34475386453796475No Hit
TTGTAGAACAGTGTATATCAATGAG64700.3341158633254392No Hit
GGAATATGGCGAGAAAACTGAAAAT57740.2981738786462265No Hit
GTGTATATCAATGAGTTACAATGAG51370.2652787001395334No Hit
CTGTAGGACATGGAATATGGCAAGA50300.2597531364029303No Hit
GCCATATTCCACGTCCTACAGTGGA48360.24973482458142565No Hit
ATTCCAGGTCCTTCAGTGTGCATTT48260.2492184167555749No Hit
CCATATTCCAGGTCCTTCAGTGTGC47120.24333136754087625No Hit
GAAATATGGCGAGGAAAACTGAAAA46710.24121409545488814No Hit
GTGTATATCAATGAGTTACAATGAA43440.22432755954956843No Hit
ACAGTGGACATTTCTAAATTTTCCA42920.2216422388551445No Hit
GTATCAACGCAGAGTACTTTTTTTT42400.21895691816072055No Hit
GTTCTACAGTGTGGTTTTTATCATT42150.21766589859609367No Hit
GTCCACTGTAGGACGTGGAATATGG41500.21430924772806378No Hit
CCATATTTCACGTCCTAAAGTGTGT41060.21203705329432043No Hit
ATACACACTTTAGGACGTGAAATAT40990.2116755678162249No Hit
TTCCAGGTCCTTCAGTGTGCATTTC40300.2081123538178547No Hit
ACCTGGAATATGGCGAGAAAACTGA38990.20134741129920977No Hit
GACCTGGAATATGGCGAGAAAACTG37780.19509887660641564No Hit
CCTAAAGTGTGTATTTCTCATTTTC37780.19509887660641564No Hit
GTGTATTTCTCATTTTCCGTGATTT36010.18595845808885725No Hit
CCACTGTAGGACGTGGAATATGGCA35250.18203375861239152No Hit
GAAATACACACTTTAGGACGTGAAA34830.17986484574381834No Hit
CACTTTAGGACGTGAAATATGGCGA34810.1797615641786482No Hit
GTTCTACAAATCCCGTTTCCAACGA32970.17025966018299427No Hit
GATATACACTGTTCTACAATGCCGG32910.16994981548748384No Hit
TCCTAAAGTGTGTATTTCTCATTTT31360.1619454941867971No Hit
CTACAGTGGACATTTCTAAATTTTC31000.16008642601373438No Hit
TATCAACGCAGAGTACTTTTTTTTT30730.15869212488393733No Hit
AGTGTGTATTTCTCATTTTCCGTGA29780.15378625053835515No Hit
GTAGGACGTGGAATATGGCAAGAAA29570.1527017941040686No Hit
ATTCCACGTCCTACAGTGGACATTT29440.15203046393046257No Hit
CTGTAGAACATATTAGATGAGTGAG28220.14573028845508335No Hit
TTTCTAAATATTCCACCTTTTTCAG26800.13839729732800263No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26150.1350406464599727No Hit
ATATTTCACGTCCTAAAGTGTGTAT25870.1335947045475906No Hit
ATGTTCTACAGTGTGGTTTTTATCA24860.12837898550649796No Hit
GGTCCTTCAGTGTGCATTTCTCATT24370.12584858715982927No Hit
GTATTTCTCATTTTCCGTGATTTTC23470.12120091672717245No Hit
GTCCTACAGTGGACATTTCTAAATA23010.11882544072825897No Hit
TTTTTCAAGTCGTCAAGTGGATGTT22670.1170696541203664No Hit
GTGCATTTCTCATTTTTCACGTTTT22090.11407448873043202No Hit
GTAGAACAGTGTATATCAATGAGTT21910.11314495464390065No Hit
GAGAAATACACACTTTAGGACGTGA21770.11242198368770959No Hit
GTAGAACATATTAGATGAGTGAGTT21490.1109760417753275No Hit
ACGTGGAATATGGCAAGAAAACTGA21320.11009814847138119No Hit
CTTTAGGACATGAAATATGGCGAGG21050.10870384734158414No Hit
CACTGAAGGACCTGGAATATGGCGA20660.1066898568207662No Hit
GTGAAATATGGCGAGGAAAACTGAA20590.10632837134267067No Hit
GTATCAACGCAGAGTACATGGGAAA20040.10348812830049152No Hit
CCTTCAGTGTGCATTTCTCATTTTT19900.10276515734430046No Hit
ACGTCCTACAGTGGACATTTCTAAA19670.10157741934484373No Hit
TTGATATACACTGTTCTACAAATCC19370.10002819586729145No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAGG400.005269865514.25401419
GGACCCG400.005283439514.2484886
TAGGACC37800.014.0944324
CCACCTT22500.013.97732413
GTCCTAT7250.013.8849521
CACCTTT23650.013.58028914
CCAACGA7000.013.57525119
TATGCCG707.2475086E-613.5745516
CGCAAGG707.2475086E-613.5745516
TTGTAGA13100.013.4114881
TATACTG1850.013.3478645
CCGGGAA500.001498933213.3009959
GGACCGG500.001500978413.298596
AGGACCG500.001502733213.2965275
ACCTTTT24450.013.25287715
TAGAAAT29800.013.2253874
TCCAACG9650.013.19542618
GTACCGC655.446041E-513.15585112
GGCGAAG1102.73576E-912.95819519
CCAACGT2500.012.9236419
Produced by FastQC (version 0.11.5)