FastQCFastQC Report
Thu 2 Feb 2017
SRR4064154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1936454
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA105270.5436225182730908No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA90840.4691048690028268No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA87890.4538708381402295No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC74540.3849303933891536No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA56890.29378441212649514No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA53270.2750904488306978No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG43670.2255152975490252No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT36840.19024464304341854No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA35010.1807943798303497No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA32790.16933012609646292No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC32590.1682973104447614No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA30750.1587954064491075No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG27380.141392462717937No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA26570.13720955932854587No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT26530.13700299619820558No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC23350.12058122733615154No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA23160.1196000524670351No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA22160.11443597420852755No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA22140.11433269264335741No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC21540.11123424568825287No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG21450.11076947864498718No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAG20750.10715462386403189No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT20620.10648329369042589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGG357.2892362E-637.71696536
AATCGCC250.002353811935.19795611
CACCGCC300.00574458729.3346632
TAGGACC45000.028.8924034
TCTCGCG407.036061E-427.49840243
TGTGGCG1300.025.38313916
TTCGTAA1151.2732926E-1124.8681244
TATAGGA9350.024.4685342
ATACCGG450.001398780224.4436556
CTCGCGA450.001398990624.44302644
GAATTCG450.001398990624.44302641
ATAGGAC9350.023.5286523
AGGACCT89050.023.4197945
GGACCTG87500.022.8792636
GGACCTA3950.022.8347056
TTTCGTA1355.456968E-1222.8134943
GGCGGAA500.00257992922.00156238
GGGCGTC602.8742696E-421.999297
ACTGTTC28900.021.9231688
CTAGGAC5900.021.6264233
Produced by FastQC (version 0.11.5)