##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064154_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1936454
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.394573276721264	32.0	32.0	32.0	32.0	32.0
2	31.545819833572086	32.0	32.0	32.0	32.0	32.0
3	31.586079504083237	32.0	32.0	32.0	32.0	32.0
4	31.649348241682993	32.0	32.0	32.0	32.0	32.0
5	31.637862298820423	32.0	32.0	32.0	32.0	32.0
6	35.25880656085815	36.0	36.0	36.0	36.0	36.0
7	35.274834826956905	36.0	36.0	36.0	36.0	36.0
8	35.21880509425992	36.0	36.0	36.0	36.0	36.0
9	35.31122247158982	36.0	36.0	36.0	36.0	36.0
10	35.215965884033395	36.0	36.0	36.0	36.0	36.0
11	35.29936419868481	36.0	36.0	36.0	36.0	36.0
12	35.25771074345169	36.0	36.0	36.0	36.0	36.0
13	35.27127935907592	36.0	36.0	36.0	36.0	36.0
14	35.23434225651629	36.0	36.0	36.0	36.0	36.0
15	35.20528347174785	36.0	36.0	36.0	36.0	36.0
16	35.23614761827547	36.0	36.0	36.0	36.0	36.0
17	35.25242376013063	36.0	36.0	36.0	36.0	36.0
18	35.24478867042543	36.0	36.0	36.0	36.0	36.0
19	35.17996916012464	36.0	36.0	36.0	36.0	36.0
20	35.16469536585945	36.0	36.0	36.0	36.0	36.0
21	35.12970047313285	36.0	36.0	36.0	36.0	36.0
22	35.08443164671095	36.0	36.0	36.0	36.0	36.0
23	35.100164527533316	36.0	36.0	36.0	36.0	36.0
24	35.07471336783626	36.0	36.0	36.0	36.0	36.0
25	35.024965736340754	36.0	36.0	36.0	36.0	36.0
26	34.99397765193493	36.0	36.0	36.0	36.0	36.0
27	34.89463111439776	36.0	36.0	36.0	36.0	36.0
28	34.824255055890816	36.0	36.0	36.0	36.0	36.0
29	34.80366226101937	36.0	36.0	36.0	32.0	36.0
30	34.706269294287395	36.0	36.0	36.0	32.0	36.0
31	34.701169767007116	36.0	36.0	36.0	32.0	36.0
32	34.6580621073364	36.0	36.0	36.0	32.0	36.0
33	34.738030441208515	36.0	36.0	36.0	32.0	36.0
34	34.686097888201836	36.0	36.0	36.0	32.0	36.0
35	34.67386986729352	36.0	36.0	36.0	32.0	36.0
36	34.57500875311265	36.0	36.0	36.0	32.0	36.0
37	34.436840224451494	36.0	36.0	36.0	32.0	36.0
38	34.37017610539677	36.0	36.0	36.0	32.0	36.0
39	34.11902322492556	36.0	36.0	36.0	32.0	36.0
40	34.257690087138656	36.0	36.0	36.0	32.0	36.0
41	34.236405305780565	36.0	36.0	36.0	32.0	36.0
42	34.23227094472681	36.0	36.0	36.0	32.0	36.0
43	34.25864750724779	36.0	36.0	36.0	32.0	36.0
44	34.08565501685039	36.0	36.0	36.0	32.0	36.0
45	34.063226908565866	36.0	36.0	36.0	32.0	36.0
46	33.80709430742997	36.0	36.0	36.0	32.0	36.0
47	33.8713772699997	36.0	36.0	36.0	32.0	36.0
48	33.77859066107431	36.0	36.0	36.0	27.0	36.0
49	33.719927248465495	36.0	36.0	36.0	27.0	36.0
50	33.32285713990624	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	3.0
20	12.0
21	37.0
22	97.0
23	374.0
24	1034.0
25	2665.0
26	6069.0
27	12361.0
28	21957.0
29	34565.0
30	50519.0
31	72281.0
32	103665.0
33	164943.0
34	331843.0
35	1134028.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.74235579627025	21.67336080538313	13.184001619607999	26.400281778738616
2	9.478333526126185	21.25567017549658	51.73828576858248	17.527710529794756
3	18.950096155633727	27.572550510110283	31.475477731346736	22.001875602909248
4	9.744718955368938	18.944111246639476	42.05093433667931	29.260235461312273
5	8.545929828438991	42.70496484811929	38.09024123475177	10.658864088689946
6	28.096413540241162	41.11208655013039	19.688450515117868	11.103049394510574
7	24.687263097665216	32.5080611344872	24.168840745858923	18.63583502198867
8	19.59865816590531	48.74647164352987	20.116976700711714	11.537893489853102
9	26.068872846593877	14.477519986738338	21.87575042256052	37.577856744107265
10	15.079777798548989	30.62726930487425	36.957438930491556	17.335513966085205
11	32.552232069545674	18.875842132062008	32.82685775133311	15.745068047059213
12	26.886687953052213	22.659827343761698	37.192923748572774	13.260560954613315
13	31.81346604415007	24.381481284480135	26.637372375404155	17.16768029596564
14	16.425967984648164	28.556231364055595	28.0351737151462	26.98262693615004
15	17.330976346991356	45.41841413654718	21.848499073782865	15.402110442678598
16	14.923157959967776	28.175517960794032	39.56084360992336	17.340480469314826
17	16.101182883765894	34.30605632770001	33.03352416323858	16.559236625295515
18	16.218466087633693	27.371572782523867	42.768637613113086	13.64132351672935
19	24.834852606726116	24.370911336815993	28.32980110015637	22.464434956301524
20	23.85980259661926	31.204255642180676	31.17895148883958	13.75699027236048
21	19.24114902806883	26.960051723407837	27.686069485771412	26.11272976275192
22	22.560458571096596	35.57138061917426	27.93627514265796	13.931885667071187
23	16.37347445806564	35.32910599485758	32.168517224622676	16.12890232245411
24	24.114473569377317	27.94659497523041	33.24041401597875	14.698517439413525
25	19.582818208014572	32.138036027270914	33.215383836787645	15.063761927926866
26	13.335629745387465	34.91773714930225	35.513960773701506	16.232672331608786
27	16.051974477556826	33.787851792174386	32.71035228924747	17.44982144102131
28	14.890272337370924	35.03900309081097	29.119828959633544	20.95089561218456
29	19.278756624385586	29.381390059729213	27.867761202970865	23.472092112914336
30	15.361928301189126	30.850794627908396	32.015081766060135	21.77219530484234
31	22.473657328099314	27.187424308232117	31.2478857949695	19.091032568699077
32	17.60675647970221	33.76158662007144	31.39657084073326	17.23508605949309
33	14.970666914551895	34.66483851310176	35.62270835269937	14.741786219646972
34	17.877453005577358	33.38432142119397	34.39945259243958	14.338772980789095
35	15.568751878487792	35.20572733497969	33.3044136587623	15.92110712777022
36	14.225002311098073	32.747046055149255	35.5636936046881	17.464258029064574
37	15.541589095143785	32.67919344551832	28.68200280419441	23.097214655143475
38	18.334838796641602	36.795382488388825	28.28360139876392	16.586177316205656
39	22.38478846792431	28.603063562650377	30.29659058456196	18.71555738486335
40	24.12859273458899	29.058029093042375	32.35439019049704	14.458987981871587
41	22.71468601818026	26.125369631881245	35.31754515886812	15.842399191070374
42	18.925924051521932	34.91332966332683	32.55464470997481	13.606101575176435
43	14.31936980808975	35.96553650901196	34.18380046539346	15.531293217504826
44	13.945707334782389	34.98090042970806	31.78311026832353	19.290281967186022
45	14.729432372681881	35.39742484748989	31.502988232462954	18.370154547365278
46	17.4817461141261	28.64348285020375	32.01328006023434	21.861490975435814
47	22.888366962470876	29.40711086873895	30.354686183134206	17.349835985655975
48	15.554739613915006	31.44380873295108	30.215248503289327	22.78620314984458
49	20.53312993718922	29.508828263663784	30.440532706482117	19.51750909266488
50	16.375460383758135	36.901552755899004	28.011885802105734	18.711101058237126
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	128.0
1	85.5
2	43.0
3	129.0
4	215.0
5	254.0
6	293.0
7	296.5
8	300.0
9	442.5
10	585.0
11	844.5
12	1104.0
13	1948.0
14	2792.0
15	3830.5
16	4869.0
17	5524.5
18	6180.0
19	6569.0
20	6958.0
21	8303.5
22	9649.0
23	12033.0
24	14417.0
25	20196.0
26	25975.0
27	42856.5
28	59738.0
29	81950.0
30	104162.0
31	132598.5
32	161035.0
33	206910.5
34	252786.0
35	296885.5
36	340985.0
37	323814.5
38	306644.0
39	259855.5
40	213067.0
41	171870.5
42	130674.0
43	103741.0
44	76808.0
45	64809.0
46	52810.0
47	46734.0
48	40658.0
49	36292.5
50	31927.0
51	28768.5
52	25610.0
53	22405.0
54	19200.0
55	16691.5
56	14183.0
57	12241.5
58	10300.0
59	8827.0
60	7354.0
61	6178.5
62	5003.0
63	4133.5
64	3264.0
65	2802.0
66	2340.0
67	2028.0
68	1716.0
69	1374.0
70	1032.0
71	835.0
72	638.0
73	518.0
74	398.0
75	321.0
76	244.0
77	200.0
78	156.0
79	120.0
80	84.0
81	65.5
82	47.0
83	36.0
84	25.0
85	20.0
86	15.0
87	11.5
88	8.0
89	9.5
90	11.0
91	9.5
92	8.0
93	6.0
94	4.0
95	6.5
96	9.0
97	6.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009450263213068837
2	3.098446955104537E-4
3	5.16407825850756E-4
4	0.0
5	0.0
6	2.065631303403024E-4
7	1.032815651701512E-4
8	0.0
9	0.0023238352163284025
10	6.713301736059828E-4
11	0.0
12	2.58203912925378E-4
13	6.713301736059828E-4
14	4.131262606806048E-4
15	0.004286184954561276
16	7.229709561910585E-4
17	0.0
18	3.6148547809552924E-4
19	1.032815651701512E-4
20	6.713301736059828E-4
21	0.0
22	2.065631303403024E-4
23	5.680486084358317E-4
24	3.6148547809552924E-4
25	0.0016525050427224192
26	0.005164078258507561
27	0.006145253127623997
28	0.0035632139983702168
29	0.0024787575640836295
30	0.0039246994764657464
31	0.007591195040006114
32	0.003150087737689612
33	0.003821417911295595
34	0.002788602259594083
35	0.002891883824764234
36	0.007487913474835963
37	0.0035632139983702168
38	0.00893385538721808
39	0.004182903389391124
40	0.0023754759989134777
41	0.0035115732157851415
42	0.0018590681730627219
43	0.001445941912382117
44	0.0011877379994567388
45	0.0012910195646268902
46	8.778933039462854E-4
47	0.0019623497382328732
48	0.0021172720859881
49	1.5492234775522684E-4
50	0.0013426603472119656
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1936454.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.65632973346691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.12813579048887	38.299001425401265
2	12.925408817011567	12.836567243147734
3	4.2409459438875485	6.317694305144676
4	1.8570943426572895	3.688659561005854
5	0.9928766331833295	2.4651304741002944
6	0.5810833409738377	1.731267958921288
7	0.4168899777946754	1.449085833996507
8	0.28280603227614354	1.1234487671454136
9	0.2051135351709219	0.9166666801719924
>10	1.1770433447262707	10.885280725422065
>50	0.10667998334747465	3.652394566210267
>100	0.07275278196753408	6.972255600685657
>500	0.007629934806066544	2.553510723545876
>1k	0.004912423779248323	4.67645386655239
>5k	5.225982743881195E-4	1.8862243948338977
>10k+	1.045196548776239E-4	0.5463578737147853
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	10527	0.5436225182730908	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	9084	0.4691048690028268	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	8789	0.4538708381402295	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	7454	0.3849303933891536	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	5689	0.29378441212649514	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	5327	0.2750904488306978	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	4367	0.2255152975490252	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	3684	0.19024464304341854	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	3501	0.1807943798303497	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	3279	0.16933012609646292	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	3259	0.1682973104447614	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	3075	0.1587954064491075	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG	2738	0.141392462717937	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	2657	0.13720955932854587	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	2653	0.13700299619820558	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	2335	0.12058122733615154	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2316	0.1196000524670351	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	2216	0.11443597420852755	No Hit
GTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAA	2214	0.11433269264335741	No Hit
GCCATATTCCACGTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTC	2154	0.11123424568825287	No Hit
CCATATTTCACGTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAG	2145	0.11076947864498718	No Hit
CCATATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAG	2075	0.10715462386403189	No Hit
ATTCCAGGTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGAT	2062	0.10648329369042589	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.164078258507561E-5	0.0
5	0.0	0.0	0.0	5.164078258507561E-5	0.0
6	0.0	0.0	0.0	5.164078258507561E-5	0.0
7	0.0	0.0	0.0	5.164078258507561E-5	0.0
8	0.0	0.0	0.0	5.164078258507561E-5	0.0
9	0.0	0.0	0.0	1.0328156517015121E-4	0.0
10	0.0	0.0	0.0	1.0328156517015121E-4	0.0
11	0.0	0.0	0.0	1.5492234775522681E-4	0.0
12	0.0	0.0	0.0	2.0656313034030243E-4	0.0
13	0.0	0.0	0.0	2.0656313034030243E-4	0.0
14	0.0	0.0	0.0	3.0984469551045363E-4	0.0
15	0.0	0.0	0.0	3.6148547809552924E-4	0.0
16	0.0	0.0	0.0	4.1312626068060486E-4	0.0
17	0.0	0.0	0.0	4.1312626068060486E-4	0.0
18	0.0	0.0	0.0	4.6476704326568047E-4	0.0
19	0.0	0.0	0.0	5.16407825850756E-4	0.0
20	0.0	0.0	0.0	8.262525213612097E-4	0.0
21	0.0	0.0	0.0	9.295340865313609E-4	0.0
22	0.0	0.0	0.0	0.001187737999456739	0.0
23	0.0	0.0	0.0	0.0017557866078925705	0.0
24	0.0	0.0	0.0	0.0029435246073493096	0.0
25	0.0	0.0	0.0	0.00340829165061499	0.0
26	0.0	0.0	0.0	0.004441107302316502	0.0
27	0.0	0.0	0.0	0.004905874345582183	0.0
28	0.0	0.0	0.0	0.0065583793883046025	0.0
29	0.0	0.0	0.0	0.010431438082185272	0.0
30	0.0	0.0	0.0	0.020036623643009337	0.0
31	0.0	0.0	0.0	0.04151918919840079	0.0
32	0.0	0.0	0.0	0.07276186266237153	0.0
33	0.0	0.0	0.0	0.09775600143354812	0.0
34	0.0	0.0	0.0	0.12419608211710684	0.0
35	0.0	0.0	0.0	0.15234030862597303	0.0
36	0.0	0.0	0.0	0.1924651966945768	0.0
37	0.0	0.0	0.0	0.26956488509409465	0.0
38	0.0	0.0	0.0	0.35895507974886054	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGGCGG	35	7.2892362E-6	37.716965	36
AATCGCC	25	0.0023538119	35.197956	11
CACCGCC	30	0.005744587	29.33466	32
TAGGACC	4500	0.0	28.892403	4
TCTCGCG	40	7.036061E-4	27.498402	43
TGTGGCG	130	0.0	25.383139	16
TTCGTAA	115	1.2732926E-11	24.86812	44
TATAGGA	935	0.0	24.468534	2
ATACCGG	45	0.0013987802	24.443655	6
CTCGCGA	45	0.0013989906	24.443026	44
GAATTCG	45	0.0013989906	24.443026	41
ATAGGAC	935	0.0	23.528652	3
AGGACCT	8905	0.0	23.419794	5
GGACCTG	8750	0.0	22.879263	6
GGACCTA	395	0.0	22.834705	6
TTTCGTA	135	5.456968E-12	22.81349	43
GGCGGAA	50	0.002579929	22.001562	38
GGGCGTC	60	2.8742696E-4	21.99929	7
ACTGTTC	2890	0.0	21.923168	8
CTAGGAC	590	0.0	21.626423	3
>>END_MODULE