FastQCFastQC Report
Thu 2 Feb 2017
SRR4064153_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064153_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences703647
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGT15450.21957032432455476No Hit
GCGCAAGACGGACCAGAGCGAAAGC15140.2151647061665864No Hit
CTATTGGAGCTGGAATTACCGCGGC14590.20734828685406176No Hit
GTATCTGATCGTCTTCGAACCTCCG14490.20592711970633001No Hit
GTATCAACGCAGAGTACTTTTTTTT14090.200242451115403No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14040.19953186754153715No Hit
GAACTACGACGGTATCTGATCGTCT12780.18162516148011718No Hit
GATTAAGAGGGACGGCCGGGGGCAT12550.17835647704033414No Hit
ATCAGATACCGTCGTAGTTCCGACC12110.17210334159031446No Hit
TCGTAGTTCCGACCATAAACGATGC11320.16087612112323368No Hit
GTCCTATTCCATTATTCCTAGCTGC11030.1567547363948116No Hit
GAATAACGCCGCCGCATCGCCAGTC10960.15575991939139938No Hit
TATCAACGCAGAGTACTTTTTTTTT10650.15135430123343097No Hit
GTCTTGCGCCGGTCCAAGAATTTCA10500.14922255051183334No Hit
ATACAGGACTCTTTCGAGGCCCTGT10450.14851196693796748No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT10180.14467481563909176No Hit
CTTTAATATACGCTATTGGAGCTGG10030.14254306491749413No Hit
GAATAATGGAATAGGACCGCGGTTC9820.13955861390725746No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG9650.1371426297561135No Hit
GGGTAGGCACACGCTGAGCCAGTCA9590.13628992946747445No Hit
GTACATGGGGAATAATTGCAATCCC9450.13430029546065003No Hit
GCTTTGAACACTCTAATTTTTTCAA9350.13287912831291826No Hit
GTCCTGTATTGTTATTTTTCGTCAC9260.1316000778799597No Hit
GTATCAACGCAGAGTACATGGGGAA9200.13074737759132066No Hit
CTCCCGACCCGGGGAGGTAGTGACG9020.1281892767254035No Hit
GTGCATGGCCGTTCTTAGTTGGTGG8990.12776292658108399No Hit
ATATTAAAGTTGCTGCAGTTAAAAA8940.12705234300721813No Hit
CTCTTAATCATGGCCTCAGTTCCGA8800.12506270900039365No Hit
CATCTAAGGGCATCACAGACCTGTT8290.11781475654696176No Hit
CTCTAGATAACCTCGGGCCGATCGC8240.11710417297309589No Hit
CTCCAATAGCGTATATTAAAGTTGC7930.11269855481512746No Hit
CTCCACTCCTGGTGGTGCCCTTCCG7640.1085771700867054No Hit
ACCATACTCCCCCCGGAACCCAAAG7390.10502425221737605No Hit
GTTCTATTTTGTTGGTTTTCGGAAC7360.10459790207305651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAAGAC504.5678244E-617.0950093
TCCAACG402.75409E-416.62842418
CGTTATT601.4695761E-615.8275882
TTTTCGG1150.015.69888316
GTTTTCG1250.014.44194615
GGTATCA3200.014.2438151
ACTCTAA1350.014.07496910
CGAACGA1152.5465852E-1114.04636916
CCGTCGT2050.013.9033239
CGCCAGT1850.013.8677218
ACTGATC551.9566939E-413.8190618
GAGGTTA903.6347046E-813.7201675
ACGTGGA707.24426E-613.5722928
CGTCGTA2100.013.57229210
TAAGACC707.2655785E-613.5684334
GTATCAA10450.013.3578371
ATCCCGT500.001497405313.30084610
GTGCGGC500.001497405313.30084611
CTAGACA500.001500618913.2970634
CCAAGAT1500.013.2951742
Produced by FastQC (version 0.11.5)