FastQCFastQC Report
Thu 2 Feb 2017
SRR4064153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences703647
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG19960.28366496268725655No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC15900.22596557648934765No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12200.17338239202327302No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA11870.16869254043575826No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT11840.16826619029143874No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT11670.16585020614029478No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11170.15874437040163605No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC11150.15846013697208972No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC10920.15519145253230668No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10800.1534860519550286No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA10150.14424846549477224No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9620.136716279611794No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC9380.1333054784572378No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG9070.12889986029926936No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC8270.1175305231174154No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA7890.11213008795603477No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG7810.11099315423784936No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT7630.10843505337193224No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG7590.10786658651283952No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG7520.10687176950942733No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA7430.10559271907646874No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA7070.10047651734463445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11650.027.3806921
CAACGGA801.3078898E-724.75059914
GTAAACG3000.022.00053227
CGCGGGA2700.021.9989744
ATTATAC602.872035E-421.998973
AACGCAG14400.021.5406576
AACGCTT3150.020.95437830
AACGGAC856.106169E-620.70638515
GTATTAT856.109927E-620.7049141
TAACGGC654.9261784E-420.30962836
TTAACGG654.9261784E-420.30962835
CGTCTTA654.92854E-420.30818615
AAACGCT3150.020.2530229
TCAACGC15350.020.2075214
ATCACGT550.00448299619.99906339
CGCTTCG3200.019.939432
TAAACGC3200.019.93798328
CAACGCA15750.019.6943155
TTCGGGC3150.019.55741935
CAAGTTA1252.2831955E-819.3604722
Produced by FastQC (version 0.11.5)