##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064153_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	703647
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.279036221287093	32.0	32.0	32.0	21.0	32.0
2	29.46905053244027	32.0	32.0	32.0	21.0	32.0
3	30.629075374442014	32.0	32.0	32.0	32.0	32.0
4	31.0416330915928	32.0	32.0	32.0	32.0	32.0
5	30.68329432229513	32.0	32.0	32.0	32.0	32.0
6	34.38422106539216	36.0	36.0	36.0	32.0	36.0
7	34.41986251629013	36.0	36.0	36.0	32.0	36.0
8	34.25628049291761	36.0	36.0	36.0	32.0	36.0
9	34.63177417085556	36.0	36.0	36.0	32.0	36.0
10	34.08019788331365	36.0	36.0	36.0	32.0	36.0
11	34.6769843401592	36.0	36.0	36.0	32.0	36.0
12	34.358640056732995	36.0	36.0	36.0	32.0	36.0
13	34.54747906265499	36.0	36.0	36.0	32.0	36.0
14	34.40003865574642	36.0	36.0	36.0	32.0	36.0
15	34.30644485089825	36.0	36.0	36.0	32.0	36.0
16	34.339521095094554	36.0	36.0	36.0	32.0	36.0
17	34.26203195636448	36.0	36.0	36.0	32.0	36.0
18	34.31392729593106	36.0	36.0	36.0	32.0	36.0
19	34.30940229973268	36.0	36.0	36.0	32.0	36.0
20	34.32464289622495	36.0	36.0	36.0	32.0	36.0
21	34.305243964658416	36.0	36.0	36.0	32.0	36.0
22	34.29199584450726	36.0	36.0	36.0	32.0	36.0
23	34.19382161794195	36.0	36.0	36.0	32.0	36.0
24	34.146616129962894	36.0	36.0	36.0	32.0	36.0
25	34.110950519223415	36.0	36.0	36.0	32.0	36.0
26	33.9830824262734	36.0	36.0	36.0	32.0	36.0
27	34.00517873308633	36.0	36.0	36.0	32.0	36.0
28	33.94395911586349	36.0	36.0	36.0	32.0	36.0
29	33.89052180994163	36.0	36.0	36.0	32.0	36.0
30	33.87096797115599	36.0	36.0	36.0	32.0	36.0
31	33.90312472020772	36.0	36.0	36.0	32.0	36.0
32	33.83260640633727	36.0	36.0	36.0	32.0	36.0
33	33.77572845475075	36.0	36.0	36.0	32.0	36.0
34	33.758614759957766	36.0	36.0	36.0	32.0	36.0
35	33.739473059644965	36.0	36.0	36.0	32.0	36.0
36	33.65094429451131	36.0	36.0	36.0	27.0	36.0
37	33.67020963636596	36.0	36.0	36.0	32.0	36.0
38	33.5989437885758	36.0	36.0	36.0	27.0	36.0
39	33.60685684725438	36.0	36.0	36.0	27.0	36.0
40	33.595355341527785	36.0	36.0	36.0	27.0	36.0
41	33.54274089138446	36.0	36.0	36.0	21.0	36.0
42	33.42802427921955	36.0	36.0	36.0	21.0	36.0
43	33.477830503078955	36.0	36.0	36.0	21.0	36.0
44	33.32615501807014	36.0	36.0	36.0	21.0	36.0
45	33.281743544703524	36.0	36.0	36.0	21.0	36.0
46	33.27736492872136	36.0	36.0	36.0	21.0	36.0
47	33.29108345519842	36.0	36.0	36.0	21.0	36.0
48	33.22256898700627	36.0	36.0	36.0	21.0	36.0
49	33.18208704080313	36.0	36.0	36.0	21.0	36.0
50	32.26770951911967	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	0.0
20	5.0
21	36.0
22	79.0
23	263.0
24	670.0
25	1579.0
26	3275.0
27	6242.0
28	11579.0
29	20033.0
30	32131.0
31	50764.0
32	78793.0
33	124825.0
34	189927.0
35	183443.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.56976372357435	18.766672588381596	12.469710428139988	24.19385325990407
2	16.80110965203995	21.170506676671728	37.02952060985385	24.998863061434477
3	18.815000724796274	25.202445547903352	29.373434937454345	26.609118789846033
4	13.176209093480113	16.922974161760088	37.53757210646816	32.36324463829165
5	16.385772979917487	35.39672591512505	31.935757560253936	16.281743544703524
6	33.95031024122925	37.556669120552094	15.85996367491608	12.633056963302568
7	30.072181079433296	30.824831200872026	20.53487046772032	18.568117251974357
8	28.073593719578138	33.1396282510975	18.741357527282858	20.045420502041505
9	27.054163753261594	13.622087305512446	18.27728226205297	41.04646667917299
10	15.369015253473709	26.130011952083652	30.58298028972078	27.917992504721855
11	38.289511644333025	20.720048547069766	22.232738859115436	18.75770094948177
12	24.826795253870195	23.45906399089316	28.5050600656295	23.20908068960715
13	28.97416875579128	19.095451677268617	25.510201181279168	26.420178385660932
14	23.876460782181972	20.21510785948068	24.564447798398913	31.343983559938437
15	24.930325498238417	27.522316448014507	21.989144847639878	25.558213206107194
16	26.043499393157315	26.104894250201095	23.390872062781927	24.460734293859662
17	23.682613583231365	26.39221086709671	24.946741761138753	24.978433788533174
18	25.20269396444524	24.85436589653619	26.054967902939968	23.887972236078603
19	25.628617758620443	24.934803957097806	25.270625754106817	24.16595253017493
20	25.74195794754457	24.05516986548615	24.492890591136156	25.709981595833124
21	27.415309096748796	24.198355141143217	23.882856034346766	24.50347972776122
22	25.85092503901109	24.4719645958337	25.092589171259412	24.584521193895796
23	24.02799134792211	23.87933705300677	25.897397270786733	26.19527432828439
24	24.47484171705903	25.24255839237115	25.24255839237115	25.04004149819867
25	24.65589305788671	24.533812370937692	25.607525514010852	25.202769057164744
26	24.541122397206443	25.674832544317304	25.7366557753557	24.047389283120552
27	25.402530659696907	24.634350514702017	24.997903671204686	24.965215154396393
28	23.95316810280505	24.800781100106164	26.19300392397633	25.05304687311245
29	24.166355185346784	24.65908784057462	25.975552387841855	25.19900458623674
30	23.98013711868839	25.123929826157156	26.36592839357384	24.53000466158062
31	25.411486810108485	24.81598392284329	24.525622968503725	25.246906298544502
32	24.975697523705573	25.047894353866795	24.26410396343543	25.7123041589922
33	23.98280912242105	24.53537304460163	25.893901473645915	25.58791635933141
34	25.174593609745976	24.52425550783504	25.92995790425136	24.371192978167628
35	26.380752602900927	24.30553779073899	25.46053510469872	23.853174501661364
36	24.057203118826376	25.492255520908248	24.982731616633362	25.46780974363202
37	25.59652650952581	25.15480767180916	24.933237637060042	24.315428181604997
38	24.53439983627247	24.491336084865438	25.737484508419463	25.23677957044263
39	25.55221411496514	24.530216508295645	24.54954506823266	25.368024308506556
40	25.816157002300915	25.200637264028263	25.371607073573866	23.611598660096956
41	24.083998811873872	25.278093995557317	26.322394329966503	24.315512862602308
42	26.36181337305067	25.761079189754977	24.908227088038043	22.968880349156315
43	25.109395895895187	24.09338906282643	25.75362011946501	25.043594921813376
44	24.501165368654426	25.387129782275025	24.61571258029674	25.495992268773804
45	24.69305993556355	25.91655664339242	24.77563068562146	24.614752735422567
46	24.322277063795134	25.228134153256693	24.796523236925545	25.65306554602263
47	25.008242851458135	24.590273435279407	25.722386447615257	24.679097265647208
48	26.372614366173565	25.401519813427054	23.982468282741575	24.243397537657803
49	24.714700289634276	25.624959141386434	24.47452270033511	25.18581786864418
50	25.507612590014457	25.327548153380224	24.21816931173318	24.946669944872145
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	4.5
2	7.0
3	16.5
4	26.0
5	52.0
6	78.0
7	85.0
8	92.0
9	107.0
10	122.0
11	180.0
12	238.0
13	428.5
14	619.0
15	852.0
16	1085.0
17	1197.0
18	1309.0
19	1460.5
20	1612.0
21	1960.0
22	2308.0
23	2798.5
24	3289.0
25	4163.5
26	5038.0
27	6479.0
28	7920.0
29	9082.0
30	10244.0
31	11333.5
32	12423.0
33	14400.0
34	16377.0
35	18996.5
36	21616.0
37	25608.5
38	29601.0
39	32005.5
40	34410.0
41	37070.0
42	39730.0
43	39025.0
44	38320.0
45	41735.5
46	45151.0
47	48090.0
48	51029.0
49	51749.5
50	52470.0
51	48846.0
52	45222.0
53	44174.5
54	43127.0
55	44892.5
56	46658.0
57	47100.5
58	47543.0
59	42285.0
60	37027.0
61	32832.0
62	28637.0
63	25131.0
64	21625.0
65	18622.5
66	15620.0
67	13767.0
68	11914.0
69	11740.5
70	11567.0
71	8888.0
72	6209.0
73	5308.0
74	4407.0
75	3522.0
76	2637.0
77	2363.5
78	2090.0
79	1786.0
80	1482.0
81	1291.5
82	1101.0
83	896.0
84	691.0
85	536.5
86	382.0
87	292.0
88	202.0
89	170.5
90	139.0
91	111.0
92	83.0
93	65.5
94	48.0
95	45.0
96	42.0
97	35.0
98	28.0
99	39.0
100	50.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0031265677250098415
2	4.2635014431952387E-4
3	1.4211671477317461E-4
4	0.0
5	0.0
6	1.4211671477317461E-4
7	0.0
8	0.0
9	4.2635014431952387E-4
10	5.684668590926985E-4
11	0.0
12	0.0
13	4.2635014431952387E-4
14	0.0
15	0.002558100865917143
16	7.105835738658731E-4
17	0.0
18	0.0
19	0.0
20	2.8423342954634923E-4
21	0.0
22	1.4211671477317461E-4
23	1.4211671477317461E-4
24	2.8423342954634923E-4
25	0.0019896340068244448
26	0.0039792680136488895
27	0.005116201731834286
28	0.003410801154556191
29	0.0028423342954634924
30	0.003268684439783016
31	0.005684668590926985
32	0.0021317507215976193
33	0.002558100865917143
34	0.0018475172920512698
35	0.0012790504329585714
36	0.006963719023885556
37	0.004547734872741588
38	0.00554255187615381
39	0.0035529178693293655
40	0.0019896340068244448
41	0.002558100865917143
42	0.001136933718185397
43	0.0014211671477317462
44	9.948170034122224E-4
45	0.001136933718185397
46	5.684668590926985E-4
47	9.948170034122224E-4
48	0.001136933718185397
49	1.4211671477317461E-4
50	0.0014211671477317462
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	703647.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.243233308751925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.1062771423109	46.74165734736789
2	10.483261708212655	11.792250681834192
3	2.8220923148888146	4.7617078945538225
4	1.1578004858337194	2.6047377139892887
5	0.6039403802733836	1.6983779856146137
6	0.3355316567261908	1.132283115103393
7	0.24321634729041444	0.957549163561005
8	0.17362746205597954	0.7812295885776743
9	0.1354727947648364	0.6857485202652623
>10	0.7300102210791132	7.8370606701807635
>50	0.09627724266505239	3.8434689480863016
>100	0.10033092023423998	11.740725498821398
>500	0.009374353658222014	3.461997839516496
>1k	0.002786970006498436	1.9612050325279085
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1996	0.28366496268725655	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1590	0.22596557648934765	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1220	0.17338239202327302	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1187	0.16869254043575826	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1184	0.16826619029143874	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1167	0.16585020614029478	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1117	0.15874437040163605	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1115	0.15846013697208972	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	1092	0.15519145253230668	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1080	0.1534860519550286	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	1015	0.14424846549477224	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	962	0.136716279611794	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	938	0.1333054784572378	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	907	0.12889986029926936	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	827	0.1175305231174154	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	789	0.11213008795603477	No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG	781	0.11099315423784936	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	763	0.10843505337193224	No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG	759	0.10786658651283952	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	752	0.10687176950942733	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	743	0.10559271907646874	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	707	0.10047651734463445	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.4211671477317461E-4	0.0
11	0.0	0.0	0.0	1.4211671477317461E-4	0.0
12	0.0	0.0	0.0	1.4211671477317461E-4	0.0
13	0.0	0.0	0.0	1.4211671477317461E-4	0.0
14	0.0	0.0	0.0	1.4211671477317461E-4	0.0
15	0.0	0.0	0.0	1.4211671477317461E-4	0.0
16	0.0	0.0	0.0	1.4211671477317461E-4	0.0
17	0.0	0.0	0.0	1.4211671477317461E-4	0.0
18	0.0	0.0	0.0	1.4211671477317461E-4	0.0
19	0.0	0.0	0.0	1.4211671477317461E-4	0.0
20	0.0	0.0	0.0	1.4211671477317461E-4	0.0
21	0.0	0.0	0.0	1.4211671477317461E-4	0.0
22	0.0	0.0	0.0	1.4211671477317461E-4	0.0
23	0.0	0.0	0.0	1.4211671477317461E-4	0.0
24	0.0	0.0	0.0	1.4211671477317461E-4	0.0
25	0.0	0.0	0.0	1.4211671477317461E-4	0.0
26	0.0	0.0	0.0	1.4211671477317461E-4	0.0
27	0.0	0.0	0.0	1.4211671477317461E-4	0.0
28	0.0	0.0	0.0	1.4211671477317461E-4	0.0
29	0.0	0.0	0.0	2.8423342954634923E-4	0.0
30	0.0	0.0	0.0	4.2635014431952387E-4	0.0
31	0.0	0.0	0.0	0.0014211671477317462	0.0
32	0.0	0.0	0.0	0.0021317507215976193	0.0
33	0.0	0.0	0.0	0.002984451010236667	0.0
34	0.0	0.0	0.0	0.0038371512988757146	0.0
35	0.0	0.0	0.0	0.005258318446607461	0.0
36	0.0	0.0	0.0	0.008242769456844127	0.0
37	0.0	0.0	0.0	0.013785321332997938	0.0
38	0.0	0.0	0.0	0.020891157071656667	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1165	0.0	27.380692	1
CAACGGA	80	1.3078898E-7	24.750599	14
GTAAACG	300	0.0	22.000532	27
CGCGGGA	270	0.0	21.99897	44
ATTATAC	60	2.872035E-4	21.99897	3
AACGCAG	1440	0.0	21.540657	6
AACGCTT	315	0.0	20.954378	30
AACGGAC	85	6.106169E-6	20.706385	15
GTATTAT	85	6.109927E-6	20.704914	1
TAACGGC	65	4.9261784E-4	20.309628	36
TTAACGG	65	4.9261784E-4	20.309628	35
CGTCTTA	65	4.92854E-4	20.308186	15
AAACGCT	315	0.0	20.25302	29
TCAACGC	1535	0.0	20.207521	4
ATCACGT	55	0.004482996	19.999063	39
CGCTTCG	320	0.0	19.9394	32
TAAACGC	320	0.0	19.937983	28
CAACGCA	1575	0.0	19.694315	5
TTCGGGC	315	0.0	19.557419	35
CAAGTTA	125	2.2831955E-8	19.36047	22
>>END_MODULE