FastQCFastQC Report
Thu 2 Feb 2017
SRR4064152_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064152_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610988
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38260.23749400988710032No Hit
TATCAACGCAGAGTACTTTTTTTTT26760.16610924476159972No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG18280.11347073969514361No Hit
GAATAGGACCGCGGTTCTATTTTGT17220.10689092656183659No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17100.1061460420561792No Hit
GTCTTGCGCCGGTCCAAGAATTTCA16320.10130429276940611No Hit
GATTAAGAGGGACGGCCGGGGGCAT16180.10043526084613913No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAACG250.006050366418.9900932
CCGCACT508.716074E-515.1996269
ACTCTAA3000.013.616755510
GTATTAA1301.4551915E-1113.1506631
TTATACT1551.8189894E-1212.255484
CGGTCCA2750.012.09098810
CCTATTC3150.012.05870153
CGCATCG2550.011.9242413
CGTCTTA1209.978976E-911.87766115
AGGACGG802.8717908E-511.8728655
TATACTT802.8717908E-511.8728655
GTATTAT1459.822543E-1111.790251
GTTCTAA1052.7284477E-711.7590591
AGACCCG752.0773699E-411.397955
GTTTTCG1850.011.29982915
GCATCGC2700.011.26143214
CTATTCC3100.011.0299324
CAGGACG951.36335E-510.9976814
ACGCGCG700.001488667110.85957513
CTAAGCT700.001494078410.8548544
Produced by FastQC (version 0.11.5)