FastQCFastQC Report
Thu 2 Feb 2017
SRR4064152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610988
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA18280.11347073969514361No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17530.10881521153478486No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17150.1064564106002031No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31250.020.9790211
ATACGAA4650.018.92398340
AATACCG951.5986185E-518.525015
ACCGTCG4200.018.332048
CGCAATA4900.017.96070536
TACGAAT4950.017.77652441
CGAATGC4950.017.33264743
TAATACT2700.017.1099034
CGTCGTA4400.016.99932710
TAGCGGC5450.016.95661530
AACGCAG38500.016.7988156
CAATACG5250.016.76228338
ATACCGT4600.016.737956
TACCGTC4650.016.5579727
TCGTTTA5050.016.55688530
CGAGATA800.001987611916.50139835
TACGCTG800.001989674816.4988375
GGTATCA16950.016.4836371
ATCGTTT5150.016.23438529
CGAGCCG5900.016.03326415
Produced by FastQC (version 0.11.5)