##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064152_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1610988
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29315860825779	32.0	32.0	32.0	32.0	32.0
2	31.369483819867064	32.0	32.0	32.0	32.0	32.0
3	31.46130014624566	32.0	32.0	32.0	32.0	32.0
4	31.546320642984305	32.0	32.0	32.0	32.0	32.0
5	31.48245176252089	32.0	32.0	32.0	32.0	32.0
6	35.070790098995154	36.0	36.0	36.0	36.0	36.0
7	35.093497903150116	36.0	36.0	36.0	36.0	36.0
8	35.039176579837964	36.0	36.0	36.0	36.0	36.0
9	35.15365601730118	36.0	36.0	36.0	36.0	36.0
10	35.00099007565544	36.0	36.0	36.0	36.0	36.0
11	35.1546057450459	36.0	36.0	36.0	36.0	36.0
12	35.058390875661395	36.0	36.0	36.0	36.0	36.0
13	35.10463827166931	36.0	36.0	36.0	36.0	36.0
14	35.04982408310925	36.0	36.0	36.0	36.0	36.0
15	35.01266489880744	36.0	36.0	36.0	36.0	36.0
16	35.0288549635379	36.0	36.0	36.0	36.0	36.0
17	34.98850208691809	36.0	36.0	36.0	36.0	36.0
18	34.98581181237849	36.0	36.0	36.0	36.0	36.0
19	34.98491360581208	36.0	36.0	36.0	36.0	36.0
20	34.97661745463033	36.0	36.0	36.0	36.0	36.0
21	34.98099427183815	36.0	36.0	36.0	36.0	36.0
22	34.95857076526951	36.0	36.0	36.0	36.0	36.0
23	34.9097783472006	36.0	36.0	36.0	32.0	36.0
24	34.87750436378173	36.0	36.0	36.0	32.0	36.0
25	34.847450756926804	36.0	36.0	36.0	32.0	36.0
26	34.7904521945539	36.0	36.0	36.0	32.0	36.0
27	34.78760425279394	36.0	36.0	36.0	32.0	36.0
28	34.76412363096435	36.0	36.0	36.0	32.0	36.0
29	34.733960153644844	36.0	36.0	36.0	32.0	36.0
30	34.71937593576116	36.0	36.0	36.0	32.0	36.0
31	34.71014185083936	36.0	36.0	36.0	32.0	36.0
32	34.67572073783293	36.0	36.0	36.0	32.0	36.0
33	34.648708742709445	36.0	36.0	36.0	32.0	36.0
34	34.636027084000624	36.0	36.0	36.0	32.0	36.0
35	34.602301196532814	36.0	36.0	36.0	32.0	36.0
36	34.57176527696048	36.0	36.0	36.0	32.0	36.0
37	34.563695074078765	36.0	36.0	36.0	32.0	36.0
38	34.52393313916677	36.0	36.0	36.0	32.0	36.0
39	34.53411943478164	36.0	36.0	36.0	32.0	36.0
40	34.50920428954157	36.0	36.0	36.0	32.0	36.0
41	34.49057845247761	36.0	36.0	36.0	32.0	36.0
42	34.42087091896401	36.0	36.0	36.0	32.0	36.0
43	34.41152882578889	36.0	36.0	36.0	32.0	36.0
44	34.3526860535274	36.0	36.0	36.0	32.0	36.0
45	34.313425053445464	36.0	36.0	36.0	32.0	36.0
46	34.29104375699881	36.0	36.0	36.0	32.0	36.0
47	34.26254944170906	36.0	36.0	36.0	32.0	36.0
48	34.217918445078425	36.0	36.0	36.0	32.0	36.0
49	34.19469480840329	36.0	36.0	36.0	32.0	36.0
50	33.584461833359406	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	4.0
19	2.0
20	9.0
21	64.0
22	149.0
23	490.0
24	1348.0
25	2923.0
26	6061.0
27	10993.0
28	18468.0
29	28064.0
30	41159.0
31	58254.0
32	84226.0
33	138407.0
34	288278.0
35	932085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.129367162944945	17.803752909165745	11.734188695830852	26.332691232058465
2	16.112935053777857	20.270096630682335	36.788189045294814	26.82877927024499
3	17.96561478302378	24.20590409706372	28.846859339918545	28.981621779993954
4	12.021194447134304	15.811849622715998	36.1165322150134	36.050423715136304
5	14.184773567525022	37.11275316762136	33.31291108313656	15.389562181717059
6	34.50890665163654	35.83666410715915	16.593181695445093	13.061247545759214
7	30.567801334089808	30.572208572886417	20.481121499504027	18.378868593519744
8	28.565593639178964	32.64526653536	19.05092964747698	19.738210177984055
9	27.704019685010046	13.57776547326707	18.165182262633987	40.5530325790889
10	15.629713774930632	26.804658063154495	31.639633264430746	25.925994897484124
11	37.76123720350493	20.774580567949606	22.21711148686396	19.2470707416815
12	24.72492001792691	23.63198687015281	28.73693501991948	22.9061580920008
13	29.70504271005395	19.244136639894126	25.190831165396943	25.85998948465498
14	23.810525858403402	19.232270939828737	25.20892497447214	31.748278227295724
15	25.36665611362522	26.980402381262763	22.139411885207743	25.51352961990428
16	25.849096542036293	25.68131016147344	23.366503680685696	25.10308961580457
17	24.164798248031644	25.791067345008155	24.930601593556254	25.11353281340395
18	25.04162057676622	24.764274212871403	25.878501435770236	24.31560377459214
19	25.669216654624368	24.81936550737808	25.302857625258536	24.208560212739012
20	26.08572313571206	23.902637964415383	24.801719701771038	25.209919198101527
21	27.151412673464982	24.026622172232194	24.244873332389812	24.577091821913015
22	26.197620587640113	23.9844418263101	24.843915465435455	24.974022120614332
23	24.595028107184714	23.884651292135946	25.40106122003059	26.119259380648753
24	24.908549511136382	24.74268784051457	25.117676744790906	25.231085903558142
25	25.15538880877788	24.17926216620038	25.268613291834242	25.396735733187498
26	24.96857348775623	25.01413805069337	25.424529502433728	24.59275895911667
27	25.548733929691043	24.52979408898187	24.674248396848945	25.247223584478146
28	24.832456403902356	24.377686346469478	25.613809217567063	25.176048032061104
29	24.771128552876938	24.56336269078828	25.43775466371354	25.22775409262124
30	24.658845497033695	24.77834191121013	25.674161523147852	24.888651068608322
31	25.259579582264035	24.600496376501205	24.659222260710646	25.48070178052411
32	25.126417031567943	24.671899228896457	24.53794000762289	25.663743731912714
33	24.584676773558364	24.29372393560426	25.162237090248148	25.959362200589233
34	25.463313860339465	24.42169181023111	25.239346497002696	24.875647832426726
35	25.987435906985983	24.101145914807503	25.27257377680112	24.63884440140539
36	24.867476880232868	24.883617417129205	24.82842919674135	25.420476505896573
37	25.8026309173448	24.73709139057719	24.70617739789847	24.75410029417954
38	24.93650934558843	24.427218386123744	25.259751425784998	25.376520842502824
39	25.591913684052397	24.497507323459466	24.588387125609668	25.32219186687847
40	25.70036487877309	24.6223647471734	24.950991529200856	24.72627884485265
41	24.65242105642148	25.135682578189357	25.355369479350117	24.85652688603905
42	25.99335548997675	24.97042125349327	24.822993083601393	24.213230172928586
43	25.14880887719675	24.18435230195182	25.102314832345208	25.564523988506227
44	24.949859308105008	24.788713859439703	24.74240626902972	25.51902056342557
45	24.991619924467585	24.919054677820238	24.812286308073926	25.277039089638254
46	24.892875166592074	24.74085504953224	24.809881742129434	25.556388041746253
47	25.047642577007164	24.605109767255925	25.27576827147144	25.071479384265473
48	25.752878736149476	25.084515348086533	24.321983922530187	24.840621993233807
49	24.787225655952064	25.286797655338773	24.564131500162322	25.361845188546837
50	25.072844492062522	25.315183560671052	24.47084544304473	25.14112650422169
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	20.0
1	24.0
2	28.0
3	75.0
4	122.0
5	157.5
6	193.0
7	203.0
8	213.0
9	286.0
10	359.0
11	563.0
12	767.0
13	1413.0
14	2059.0
15	2840.5
16	3622.0
17	4009.5
18	4397.0
19	4469.5
20	4542.0
21	5114.5
22	5687.0
23	6402.0
24	7117.0
25	7858.0
26	8599.0
27	10478.5
28	12358.0
29	14893.0
30	17428.0
31	20212.0
32	22996.0
33	26333.0
34	29670.0
35	34398.0
36	39126.0
37	47687.5
38	56249.0
39	61940.5
40	67632.0
41	74829.0
42	82026.0
43	85640.5
44	89255.0
45	98173.5
46	107092.0
47	114608.5
48	122125.0
49	126116.5
50	130108.0
51	125817.0
52	121526.0
53	119469.0
54	117412.0
55	118230.0
56	119048.0
57	115313.0
58	111578.0
59	101397.0
60	91216.0
61	79841.0
62	68466.0
63	59728.5
64	50991.0
65	43761.5
66	36532.0
67	31296.5
68	26061.0
69	23569.0
70	21077.0
71	16298.0
72	11519.0
73	9871.0
74	8223.0
75	6390.5
76	4558.0
77	4025.0
78	3492.0
79	2867.0
80	2242.0
81	1851.0
82	1460.0
83	1181.5
84	903.0
85	664.0
86	425.0
87	300.5
88	176.0
89	132.5
90	89.0
91	76.0
92	63.0
93	51.0
94	39.0
95	48.0
96	57.0
97	36.5
98	16.0
99	22.5
100	29.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008131655853426593
2	5.586633792430484E-4
3	3.1036854402391575E-4
4	0.0
5	0.0
6	6.207370880478315E-5
7	1.241474176095663E-4
8	6.207370880478315E-5
9	9.931793408765304E-4
10	0.0011173267584860967
11	0.0
12	1.241474176095663E-4
13	5.586633792430484E-4
14	1.8622112641434945E-4
15	0.004841749286773086
16	6.828107968526147E-4
17	0.0
18	3.724422528286989E-4
19	0.0
20	6.828107968526147E-4
21	0.0
22	1.241474176095663E-4
23	7.448845056573978E-4
24	4.3451596163348206E-4
25	0.0018001375553387115
26	0.005400412666016134
27	0.006083223462868749
28	0.003972717363506122
29	0.002482948352191326
30	0.003786496237091772
31	0.0070764028037452785
32	0.0031036854402391578
33	0.004593454451553953
34	0.002420874643386543
35	0.0029795380226295912
36	0.008504098106255293
37	0.004158938489920471
38	0.007510918765378761
39	0.004283085907530037
40	0.002607095769800892
41	0.003662348819482206
42	0.0021105060993626273
43	0.0018001375553387115
44	0.001303547884900446
45	0.0014897690113147956
46	0.0010552530496813137
47	0.0021105060993626273
48	0.00235880093458176
49	5.586633792430484E-4
50	0.0013656215937052293
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1610988.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.93388986982472
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.64209097900012	43.42824166082303
2	13.410122816434878	15.00160665510584
3	4.086137627461848	6.856607161421937
4	1.742136328364743	3.897778461158973
5	0.8805696071676109	2.4626841715013987
6	0.47719597682193204	1.6014856328328824
7	0.31895230897087334	1.2488170326592192
8	0.2488897358938885	1.1137096861774813
9	0.17826789212143934	0.8974094980721744
>10	0.8753360142921789	8.553194412691177
>50	0.06770568240617295	2.62833242274726
>100	0.061761873813943206	7.256151119032937
>500	0.008264470479663924	3.157200526508818
>1k	0.002568686770706355	1.896781559267006
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1828	0.11347073969514361	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1753	0.10881521153478486	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1715	0.1064564106002031	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8622112641434945E-4
2	0.0	0.0	0.0	0.0	1.8622112641434945E-4
3	0.0	0.0	0.0	0.0	1.8622112641434945E-4
4	0.0	0.0	0.0	0.0	1.8622112641434945E-4
5	0.0	0.0	0.0	6.207370880478315E-5	1.8622112641434945E-4
6	0.0	0.0	0.0	6.207370880478315E-5	1.8622112641434945E-4
7	0.0	0.0	0.0	6.207370880478315E-5	1.8622112641434945E-4
8	0.0	0.0	0.0	6.207370880478315E-5	1.8622112641434945E-4
9	0.0	0.0	0.0	1.241474176095663E-4	2.482948352191326E-4
10	0.0	0.0	0.0	1.241474176095663E-4	2.482948352191326E-4
11	0.0	0.0	0.0	1.241474176095663E-4	2.482948352191326E-4
12	0.0	0.0	0.0	1.241474176095663E-4	3.1036854402391575E-4
13	0.0	0.0	0.0	1.241474176095663E-4	4.965896704382652E-4
14	0.0	0.0	0.0	1.241474176095663E-4	5.586633792430484E-4
15	0.0	0.0	0.0	1.241474176095663E-4	7.448845056573978E-4
16	0.0	0.0	0.0	1.241474176095663E-4	8.06958214462181E-4
17	0.0	0.0	0.0	1.8622112641434945E-4	8.06958214462181E-4
18	0.0	0.0	0.0	3.1036854402391575E-4	8.06958214462181E-4
19	0.0	0.0	0.0	3.724422528286989E-4	8.06958214462181E-4
20	0.0	0.0	0.0	4.3451596163348206E-4	8.690319232669641E-4
21	0.0	0.0	0.0	4.965896704382652E-4	9.931793408765304E-4
22	0.0	0.0	0.0	5.586633792430484E-4	9.931793408765304E-4
23	0.0	0.0	0.0	9.311056320717473E-4	0.0010552530496813137
24	0.0	0.0	0.0	0.002048432390557844	0.0010552530496813137
25	0.0	0.0	0.0	0.00235880093458176	0.0010552530496813137
26	0.0	0.0	0.0	0.002793316896215242	0.0010552530496813137
27	0.0	0.0	0.0	0.003600275110677423	0.00117940046729088
28	0.0	0.0	0.0	0.005090044121992219	0.00117940046729088
29	0.0	0.0	0.0	0.00943520373832704	0.00117940046729088
30	0.0	0.0	0.0	0.018746260059044513	0.00117940046729088
31	0.0	0.0	0.0	0.037927036079722504	0.00117940046729088
32	0.0	0.0	0.0	0.05810099144127703	0.00117940046729088
33	0.0	0.0	0.0	0.07690932520912633	0.00117940046729088
34	0.0	0.0	0.0	0.09925586037884826	0.00117940046729088
35	0.0	0.0	0.0	0.12271972230705629	0.00117940046729088
36	0.0	0.0	0.0	0.16083297951319314	0.00117940046729088
37	0.0	0.0	0.0	0.2147750324645497	0.00117940046729088
38	0.0	0.0	0.0	0.2899462938271421	0.0014276953025100126
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3125	0.0	20.979021	1
ATACGAA	465	0.0	18.923983	40
AATACCG	95	1.5986185E-5	18.52501	5
ACCGTCG	420	0.0	18.33204	8
CGCAATA	490	0.0	17.960705	36
TACGAAT	495	0.0	17.776524	41
CGAATGC	495	0.0	17.332647	43
TAATACT	270	0.0	17.109903	4
CGTCGTA	440	0.0	16.999327	10
TAGCGGC	545	0.0	16.956615	30
AACGCAG	3850	0.0	16.798815	6
CAATACG	525	0.0	16.762283	38
ATACCGT	460	0.0	16.73795	6
TACCGTC	465	0.0	16.557972	7
TCGTTTA	505	0.0	16.556885	30
CGAGATA	80	0.0019876119	16.501398	35
TACGCTG	80	0.0019896748	16.498837	5
GGTATCA	1695	0.0	16.483637	1
ATCGTTT	515	0.0	16.234385	29
CGAGCCG	590	0.0	16.033264	15
>>END_MODULE