FastQCFastQC Report
Thu 2 Feb 2017
SRR4064151_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064151_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605802
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29720.185078857791932No Hit
TATCAACGCAGAGTACTTTTTTTTT18850.11738682602213722No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT551.9575158E-413.82042612
AACCGCG1550.013.484387
GTAATAC1052.0037987E-812.6620143
CTAAGAC1001.4494435E-712.3454643
AGAACCG2200.012.0891055
TCTAGAC1650.012.0864693
GTATCAA21700.011.9921071
GAACCGC2250.011.8211966
GTATTAT1157.100061E-811.5620511
TCGCGTA1251.830449E-811.400439
ACGGTAT1604.5474735E-1111.2816759
GCGTCCC1104.9575465E-711.22909516
ACCGTCG2050.011.1227188
GCGTAAC1303.2619937E-810.96229411
CGCGGTC1658.0035534E-1110.93980710
CGTCGTA2000.010.92541110
CGATTCT700.001490203210.8582314
ACGGTGC700.001490590710.8578918
CTAGGAC1752.0008883E-1110.8531553
CCGCGCG803.7695025E-410.6879039
Produced by FastQC (version 0.11.5)