##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064151_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1605802
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22229079301184	32.0	32.0	32.0	32.0	32.0
2	30.73137099094409	32.0	32.0	32.0	32.0	32.0
3	30.74185671707969	32.0	32.0	32.0	32.0	32.0
4	30.777145002933114	32.0	32.0	32.0	32.0	32.0
5	30.641877392106874	32.0	32.0	32.0	32.0	32.0
6	34.32528294272893	36.0	36.0	36.0	32.0	36.0
7	34.25519522332143	36.0	36.0	36.0	32.0	36.0
8	34.19846344692559	36.0	36.0	36.0	32.0	36.0
9	34.39247117639659	36.0	36.0	36.0	32.0	36.0
10	33.99619878415894	36.0	36.0	36.0	32.0	36.0
11	34.37607936719471	36.0	36.0	36.0	32.0	36.0
12	34.149756321140465	36.0	36.0	36.0	32.0	36.0
13	34.24547796054557	36.0	36.0	36.0	32.0	36.0
14	34.12500358076525	36.0	36.0	36.0	32.0	36.0
15	34.04560088977346	36.0	36.0	36.0	32.0	36.0
16	34.05200018433157	36.0	36.0	36.0	32.0	36.0
17	33.97032573131681	36.0	36.0	36.0	32.0	36.0
18	33.958769512056904	36.0	36.0	36.0	32.0	36.0
19	33.98840641623313	36.0	36.0	36.0	32.0	36.0
20	33.96695607553111	36.0	36.0	36.0	32.0	36.0
21	33.96066825175208	36.0	36.0	36.0	32.0	36.0
22	33.92927397026533	36.0	36.0	36.0	32.0	36.0
23	33.86346075045367	36.0	36.0	36.0	32.0	36.0
24	33.84918377234553	36.0	36.0	36.0	32.0	36.0
25	33.419744775507816	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	16.0
5	19.0
6	69.0
7	31.0
8	109.0
9	94.0
10	135.0
11	46.0
12	89.0
13	74.0
14	152.0
15	256.0
16	414.0
17	585.0
18	836.0
19	1176.0
20	1777.0
21	2739.0
22	4366.0
23	6748.0
24	10177.0
25	14454.0
26	20324.0
27	26273.0
28	35605.0
29	46834.0
30	61512.0
31	83263.0
32	116854.0
33	163346.0
34	340206.0
35	667219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.63401164330012	17.010805406129396	11.331254691739112	26.02392825883137
2	17.124257657585844	19.4236508300285	37.43610374858009	26.015987763805576
3	19.13172036147077	23.75690710953541	28.299890457742162	28.811482071251664
4	12.84210211653985	15.643998321017055	35.758475563200385	35.755423999242716
5	14.928720455890138	36.29078566312708	33.5600535608632	15.220440320119577
6	34.15752815049139	35.44305531939807	16.91246768275631	13.486948847354238
7	30.322256743995997	30.17581604407049	20.73932932526404	18.762597886669475
8	28.22272128379957	32.88316040953505	19.064062271256233	19.830056035409147
9	27.678088558917572	14.019419459833967	18.094590115315505	40.20790186593295
10	16.110648983266817	26.673146841382145	31.421557173522256	25.79464700182878
11	37.291648103920636	20.990182046797436	22.254982656423977	19.463187192857948
12	24.946488892433795	23.364803368878626	28.817172407647774	22.87153533103981
13	29.956374544568565	19.556180625960792	24.69857558307998	25.788869246390668
14	23.851108869146024	19.355622676029743	25.039398832087134	31.753869622737096
15	25.71315520550335	26.684529298870917	21.946113008150345	25.656202487475387
16	25.88579273144897	25.741034890814703	23.325828525208614	25.047343852527714
17	24.389673965196863	25.511684336595636	24.819572307542757	25.279069390664745
18	24.89519214410044	25.146718534117163	25.560029490160495	24.3980598316219
19	25.969844912061163	24.695975042346	24.85812999567504	24.4760500499178
20	26.3037579289946	24.20516853904574	24.500266117562887	24.990807414396773
21	27.14402519721156	24.017681422789916	24.051084928413047	24.78720845158548
22	26.309311399766795	24.13039237742918	24.38247965384584	25.17781656895818
23	25.162261780261343	24.10336888737094	24.89482744221586	25.839541890151864
24	25.291214547421653	24.665565231373083	24.665004334486497	25.378215886718774
25	25.36587889148496	24.360868798381613	24.57762590515412	25.695626404979304
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	78.0
1	78.0
2	235.5
3	393.0
4	393.0
5	393.0
6	928.0
7	1463.0
8	1463.0
9	1463.0
10	1757.0
11	2051.0
12	2051.0
13	2051.0
14	2746.0
15	3441.0
16	3441.0
17	3441.0
18	6118.0
19	8795.0
20	8795.0
21	8795.0
22	14709.0
23	20623.0
24	20623.0
25	20623.0
26	31162.5
27	41702.0
28	41702.0
29	41702.0
30	53338.0
31	64974.0
32	64974.0
33	64974.0
34	80474.0
35	95974.0
36	95974.0
37	95974.0
38	111831.0
39	127688.0
40	127688.0
41	127688.0
42	147326.0
43	166964.0
44	166964.0
45	166964.0
46	187028.0
47	207092.0
48	207092.0
49	207092.0
50	213961.0
51	220830.0
52	220830.0
53	220830.0
54	209223.5
55	197617.0
56	197617.0
57	197617.0
58	180487.5
59	163358.0
60	163358.0
61	163358.0
62	142547.0
63	121736.0
64	121736.0
65	121736.0
66	100310.5
67	78885.0
68	78885.0
69	78885.0
70	59342.0
71	39799.0
72	39799.0
73	39799.0
74	30509.5
75	21220.0
76	21220.0
77	21220.0
78	16672.5
79	12125.0
80	12125.0
81	12125.0
82	8333.0
83	4541.0
84	4541.0
85	4541.0
86	3394.0
87	2247.0
88	2247.0
89	2247.0
90	1600.5
91	954.0
92	954.0
93	954.0
94	674.0
95	394.0
96	394.0
97	394.0
98	626.0
99	858.0
100	858.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.036554942639254406
2	0.004608289191319976
3	0.001805951169571342
4	0.004234644121753492
5	0.009465675095684276
6	0.014634431891353978
7	0.023290542669644202
8	0.03530945907403279
9	0.050691181104519735
10	0.05648267968280025
11	0.05511264776105647
12	0.06196280736977535
13	0.06258554915238616
14	0.06594835477848453
15	0.05990775948715969
16	0.06557470970891804
17	0.06252327497412509
18	0.07466673973503582
19	0.072487143495898
20	0.07771817446982877
21	0.07348353034807528
22	0.07466673973503582
23	0.08070733502636065
24	0.07653496508286825
25	0.07728225522200122
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1605802.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.09648792493045
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.93787319636611	48.640819192149685
2	12.044298995870566	14.47640138339175
3	3.1999943675651994	5.7692526861068245
4	1.2923386998231468	3.1066006827536823
5	0.6540572441587802	1.9653271637900716
6	0.4059869904944739	1.4639035363158002
7	0.26847528935692794	1.1294095389486578
8	0.1889948098016371	0.908633944409489
9	0.13855697170542092	0.7494108639308782
>10	0.7309027679848068	8.092737031084903
>50	0.06757936914010411	2.8631645747949195
>100	0.06489950380417064	7.854116832057277
>500	0.005000105320312308	2.1163799612020675
>1k	0.0010416886083983977	0.8638426090640201
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2972	0.185078857791932	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1885	0.11738682602213722	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.113708913054038E-4
2	0.0	0.0	0.0	0.0	3.113708913054038E-4
3	0.0	0.0	0.0	0.0	3.113708913054038E-4
4	0.0	0.0	0.0	0.0	3.113708913054038E-4
5	0.0	0.0	0.0	0.0	3.113708913054038E-4
6	0.0	0.0	0.0	0.0	3.7364506956648455E-4
7	0.0	0.0	0.0	0.0	3.7364506956648455E-4
8	0.0	0.0	0.0	0.0	4.98193426088646E-4
9	0.0	0.0	0.0	0.0	4.98193426088646E-4
10	0.0	0.0	0.0	0.0	4.98193426088646E-4
11	0.0	0.0	0.0	0.0	4.98193426088646E-4
12	0.0	0.0	0.0	0.0	8.095643173940499E-4
13	0.0	0.0	0.0	0.0	8.718384956551305E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGATT	55	1.9575158E-4	13.820426	12
AACCGCG	155	0.0	13.48438	7
GTAATAC	105	2.0037987E-8	12.662014	3
CTAAGAC	100	1.4494435E-7	12.345464	3
AGAACCG	220	0.0	12.089105	5
TCTAGAC	165	0.0	12.086469	3
GTATCAA	2170	0.0	11.992107	1
GAACCGC	225	0.0	11.821196	6
GTATTAT	115	7.100061E-8	11.562051	1
TCGCGTA	125	1.830449E-8	11.40043	9
ACGGTAT	160	4.5474735E-11	11.281675	9
GCGTCCC	110	4.9575465E-7	11.229095	16
ACCGTCG	205	0.0	11.122718	8
GCGTAAC	130	3.2619937E-8	10.962294	11
CGCGGTC	165	8.0035534E-11	10.939807	10
CGTCGTA	200	0.0	10.925411	10
CGATTCT	70	0.0014902032	10.85823	14
ACGGTGC	70	0.0014905907	10.857891	8
CTAGGAC	175	2.0008883E-11	10.853155	3
CCGCGCG	80	3.7695025E-4	10.687903	9
>>END_MODULE