FastQCFastQC Report
Thu 2 Feb 2017
SRR4064151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605802
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23250.024.6020451
GGTATCA10650.023.7557681
CTATACT1653.6379788E-1219.9980474
CGATATA1404.0763553E-918.85999936
TACGACA851.4322741E-418.1158774
AACGCAG32850.017.2098876
ATCAACG33500.016.8759633
TCAACGC33750.016.7509574
CAACGCA34300.016.4823555
TATCAAC35200.016.3103982
TATTACC1351.0870972E-616.2947044
CCGGTTA850.00298245115.52789514
GATATAT1857.572453E-915.4593941
GTCCTAA2001.2769306E-915.3999351
TACGCTA3050.015.1465339
GTATTAC1603.8258622E-715.1249361
CTTATAG2051.8135324E-915.02292253
TAGGACA2509.094947E-1214.9585394
CTATAAG2102.5447662E-914.6666051
GTATAAT1951.5168553E-814.6666051