FastQCFastQC Report
Thu 2 Feb 2017
SRR4064150_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064150_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538826
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33100.2150990430367046No Hit
TATCAACGCAGAGTACTTTTTTTTT24490.15914729800510258No Hit
GATTAAGAGGGACGGCCGGGGGCAT17310.11248835150952738No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17260.1121634284837922No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17230.11196847466835107No Hit
CTATTGGAGCTGGAATTACCGCGGC15630.10157093784482457No Hit
GAATAGGACCGCGGTTCTATTTTGT15540.1009860763985012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAC350.002168920616.28713611
CGTTATT1600.013.6481192
TCGCGTA1205.0931703E-1113.4599469
GTTATTC1850.013.3455713
CGCGTAA1251.0186341E-1012.92070710
CGTCGTA2600.012.78916210
CCGTCGT2550.012.6681849
CGCATCG1900.012.50149813
GCGTAAC1301.9826984E-1012.42416111
GTATAAA1301.9826984E-1012.4181021
GCATCGC2000.012.35188114
GTCGTAG2700.012.31588911
GCGTTAT1700.012.2891951
CCGATAA1103.806963E-812.0923599
TTTTCGG2300.011.98008516
CGAACGA1052.7142232E-711.76369716
CCCAATT658.015275E-411.69370813
TCGGGGG1552.5465852E-1111.6382272
ACTCTAA2300.011.5658510
CGATAAC1157.066774E-811.5658510
Produced by FastQC (version 0.11.5)