##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064150_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1538826
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.242416621502365	32.0	32.0	32.0	32.0	32.0
2	30.782820149906488	32.0	32.0	32.0	32.0	32.0
3	30.804434679424443	32.0	32.0	32.0	32.0	32.0
4	30.841965238435016	32.0	32.0	32.0	32.0	32.0
5	30.721705377995953	32.0	32.0	32.0	32.0	32.0
6	34.39077712489911	36.0	36.0	36.0	32.0	36.0
7	34.32323602538558	36.0	36.0	36.0	32.0	36.0
8	34.27714309480084	36.0	36.0	36.0	32.0	36.0
9	34.45057920778567	36.0	36.0	36.0	32.0	36.0
10	34.09038513776086	36.0	36.0	36.0	32.0	36.0
11	34.42655309957071	36.0	36.0	36.0	32.0	36.0
12	34.20107471539992	36.0	36.0	36.0	32.0	36.0
13	34.308360399421375	36.0	36.0	36.0	32.0	36.0
14	34.200783584368864	36.0	36.0	36.0	32.0	36.0
15	34.120461312715015	36.0	36.0	36.0	32.0	36.0
16	34.12438183394354	36.0	36.0	36.0	32.0	36.0
17	34.03566939991916	36.0	36.0	36.0	32.0	36.0
18	34.04283200309847	36.0	36.0	36.0	32.0	36.0
19	34.0649904537615	36.0	36.0	36.0	32.0	36.0
20	34.03376015221994	36.0	36.0	36.0	32.0	36.0
21	34.018187891288555	36.0	36.0	36.0	32.0	36.0
22	34.00471918202578	36.0	36.0	36.0	32.0	36.0
23	33.93122809206499	36.0	36.0	36.0	32.0	36.0
24	33.925046106577355	36.0	36.0	36.0	32.0	36.0
25	33.51856870107471	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	9.0
5	14.0
6	66.0
7	17.0
8	93.0
9	124.0
10	128.0
11	55.0
12	104.0
13	99.0
14	147.0
15	267.0
16	377.0
17	526.0
18	735.0
19	949.0
20	1598.0
21	2314.0
22	3698.0
23	5946.0
24	8861.0
25	12606.0
26	18208.0
27	23796.0
28	32384.0
29	43726.0
30	57396.0
31	77905.0
32	109806.0
33	155237.0
34	326602.0
35	655032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.45906671884934	17.235821935708685	11.277439789466294	26.027671555975672
2	16.594410625309912	19.851748929806902	37.639212836988165	25.914627607895024
3	18.667411831220228	24.145021152708914	28.7302525977866	28.45731441828426
4	12.53623018874791	15.476496842937095	36.1512296818502	35.83604328646479
5	14.53934425909883	36.76511207282573	33.7028908413472	14.992652826728243
6	33.947642073519475	35.76457130972807	17.05824624343576	13.229540373316695
7	30.133320506762225	30.457668090372724	21.03527328721401	18.37373811565104
8	28.10264531278035	32.90778979995683	19.593789898769266	19.395774988493553
9	27.856755078621514	13.768923940145573	18.423148566543027	39.951172414689886
10	15.935933748962878	26.5362427401379	31.756841111431893	25.77098239946733
11	37.1955041509748	20.720022471442544	22.966581531803335	19.117891845779322
12	24.979467567861068	23.52863908503711	29.000767974738118	22.491125372363705
13	29.746172824092316	19.313989041616107	25.260401425916463	25.679436708375114
14	23.66309803980223	19.84037748585111	25.141098733021494	31.355425741325167
15	25.1348780229291	27.644386297680363	22.063359020359965	25.157376659030568
16	25.663871043679737	25.428528880625795	24.193681592913332	24.713918482781132
17	24.01509136557741	25.817367685567937	25.32310075281256	24.844440196042093
18	24.782861204817085	24.70462295079835	26.483226251633212	24.02928959275135
19	25.851577117504863	24.578463067270967	25.161301651159206	24.408658164064963
20	25.952051738648024	24.23324015624614	24.857925537242433	24.9567825678634
21	26.996226650693995	23.933583329106035	24.177075569741927	24.893114450458043
22	26.127992289166656	24.363657885101038	24.65457014510493	24.853779680627376
23	24.55261846813858	24.16328564125445	25.375706828348843	25.90838906225813
24	25.058695903838874	24.71230877492381	25.29458514840796	24.93441017282936
25	25.003186826051326	24.456028304218318	25.229906736560046	25.310878133170306
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	189.0
1	189.0
2	402.0
3	615.0
4	615.0
5	615.0
6	1580.0
7	2545.0
8	2545.0
9	2545.0
10	2622.5
11	2700.0
12	2700.0
13	2700.0
14	3212.5
15	3725.0
16	3725.0
17	3725.0
18	6467.5
19	9210.0
20	9210.0
21	9210.0
22	15364.0
23	21518.0
24	21518.0
25	21518.0
26	32958.0
27	44398.0
28	44398.0
29	44398.0
30	56516.0
31	68634.0
32	68634.0
33	68634.0
34	83554.5
35	98475.0
36	98475.0
37	98475.0
38	112037.5
39	125600.0
40	125600.0
41	125600.0
42	143104.5
43	160609.0
44	160609.0
45	160609.0
46	179300.5
47	197992.0
48	197992.0
49	197992.0
50	202934.5
51	207877.0
52	207877.0
53	207877.0
54	193767.0
55	179657.0
56	179657.0
57	179657.0
58	164961.5
59	150266.0
60	150266.0
61	150266.0
62	132010.5
63	113755.0
64	113755.0
65	113755.0
66	93760.5
67	73766.0
68	73766.0
69	73766.0
70	55500.0
71	37234.0
72	37234.0
73	37234.0
74	28224.5
75	19215.0
76	19215.0
77	19215.0
78	15505.5
79	11796.0
80	11796.0
81	11796.0
82	8071.0
83	4346.0
84	4346.0
85	4346.0
86	3338.0
87	2330.0
88	2330.0
89	2330.0
90	1708.5
91	1087.0
92	1087.0
93	1087.0
94	772.0
95	457.0
96	457.0
97	457.0
98	643.5
99	830.0
100	830.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.041590147294106025
2	0.005523691437498457
3	0.0010397536823526505
4	0.0035091686779401957
5	0.009032860115438654
6	0.012087136557349564
7	0.022939565616905355
8	0.03626140967204869
9	0.04945328451689795
10	0.059655867524983336
11	0.056796544898513546
12	0.06595937422424628
13	0.06550448198821698
14	0.06972848132277464
15	0.062060297915423834
16	0.06920860448159831
17	0.06452971291101138
18	0.0789562952536544
19	0.07661684946836095
20	0.08136072564409491
21	0.0779815261764488
22	0.0811657718286538
23	0.08603961721468184
24	0.08058091038233042
25	0.08097081801321267
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1538826.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.916353721454776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.55803712176342	38.47139744590517
2	14.166933305969767	14.426571747100308
3	4.5370075600513236	6.930236452934629
4	1.967818684144558	4.007766087263681
5	1.0238212220882048	2.606462174568757
6	0.6100205032127792	1.863601183135302
7	0.4075749609891764	1.4526561617213125
8	0.2867294343457457	1.1679373841200507
9	0.21097688774583293	0.9667956459166639
>10	1.0553082868457224	9.585787701041266
>50	0.077423784249637	2.7571185484751424
>100	0.08283318773226876	8.954321110858594
>500	0.011411904270132046	3.9085250873554043
>1k	0.004103156591508151	2.9008232696038774
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3310	0.2150990430367046	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2449	0.15914729800510258	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1731	0.11248835150952738	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1726	0.1121634284837922	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1723	0.11196847466835107	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1563	0.10157093784482457	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1554	0.1009860763985012	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	2.599384205881627E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGTAC	35	0.0021689206	16.287136	11
CGTTATT	160	0.0	13.648119	2
TCGCGTA	120	5.0931703E-11	13.459946	9
GTTATTC	185	0.0	13.345571	3
CGCGTAA	125	1.0186341E-10	12.920707	10
CGTCGTA	260	0.0	12.789162	10
CCGTCGT	255	0.0	12.668184	9
CGCATCG	190	0.0	12.501498	13
GCGTAAC	130	1.9826984E-10	12.424161	11
GTATAAA	130	1.9826984E-10	12.418102	1
GCATCGC	200	0.0	12.351881	14
GTCGTAG	270	0.0	12.315889	11
GCGTTAT	170	0.0	12.289195	1
CCGATAA	110	3.806963E-8	12.092359	9
TTTTCGG	230	0.0	11.980085	16
CGAACGA	105	2.7142232E-7	11.763697	16
CCCAATT	65	8.015275E-4	11.693708	13
TCGGGGG	155	2.5465852E-11	11.638227	2
ACTCTAA	230	0.0	11.56585	10
CGATAAC	115	7.066774E-8	11.56585	10
>>END_MODULE