FastQCFastQC Report
Thu 2 Feb 2017
SRR4064150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1538826
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17580.1142429358484975No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16950.11014890572423393No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16610.10793942914923454No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA15650.10170090705511864No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT654.928536E-420.31042331
GTATCAA24650.020.2570931
GTCCTAC6650.018.8547761
CGTCTTA1658.367351E-1118.6649315
GAGTACG600.00741558818.3322247
GTACGTA600.00741558818.33222411
TTACGCC600.00741697818.3316294
GGCGTTA4950.018.2217142
GTCTTAC1601.1041266E-917.8727571
GTCCTAA4350.017.6989271
TTAACGG1509.586074E-917.60236735
TTACGTT750.001290205917.60179332
TAGGACG5500.017.5983624
CGTTATT5050.017.42412244
GTAAGAC1651.6625563E-917.3311583
TTATACC1408.385359E-817.2841054
TCTATAC1154.2795327E-617.2152293
GATATAC3200.017.1853431
CGCAATA3850.017.14460436
GTATTAG3600.017.1089631
Produced by FastQC (version 0.11.5)