FastQCFastQC Report
Thu 2 Feb 2017
SRR4064149_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064149_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTT190.7661290322580645No Hit
GTATCAACGCAGAGTACTTTTTTTT170.685483870967742No Hit
GAGTACTTTTTTTTTTTTTTTTTTT80.3225806451612903No Hit
ACGCAGAGTACTTTTTTTTTTTTTT80.3225806451612903No Hit
GGTATCAACGCAGAGTACTTTTTTT60.24193548387096775No Hit
GTCCTAAAGTGTGTATTTCTCATTT50.20161290322580644No Hit
GTCCTACAGTGGACATTTCTAAATT40.16129032258064516No Hit
TCCTAAAGTGTGTATTTCTCATTTT40.16129032258064516No Hit
GTGTATATCAATGAGTTACAATGAA40.16129032258064516No Hit
CTGTAGGACGTGGAATATGGCAAGA40.16129032258064516No Hit
CTGTAGGACCTGGAATATGGCGAGA40.16129032258064516No Hit
CTGTAGGACATGGAATATGGCAAGA40.16129032258064516No Hit
CTGAAGGACCTGGAATATGGCGAGA30.12096774193548387No Hit
CTCCGAGGTCACCCCAACCGAAATT30.12096774193548387No Hit
ATTTAGAAATGTCCACTGTAGGACG30.12096774193548387No Hit
AACCTAGACTTACAAGTCAAAGTAA30.12096774193548387No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT30.12096774193548387No Hit
GGCGGTGACTCGCCTCGCGGCGGAC30.12096774193548387No Hit
TTTCTAAATATTCCACCTTTTTCAG30.12096774193548387No Hit
CTTTAGGACGTGAAATATGGCGAGG30.12096774193548387No Hit
GTGTAACTCACTCATCTAAAATGTT30.12096774193548387No Hit
GTACATGGGGCAGTGGTATCAACGC30.12096774193548387No Hit
ATCAACGCAGAGTACTTTTTTTTTT30.12096774193548387No Hit
GTGTGGTTTTTATCATTTTCCATGT30.12096774193548387No Hit
GTCCTTCAGTGTGCATTTCTCATTT30.12096774193548387No Hit
TCCTTGGATGTGGTAGCCGTTTCTC30.12096774193548387No Hit
GTCCTATTCCATTATTCCTAGCTGC30.12096774193548387No Hit
GTAGAACATTTTAGATGAGTGAGTT30.12096774193548387No Hit
GTTATATAATTTAAGCTCCATAGGG30.12096774193548387No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)