FastQCFastQC Report
Thu 2 Feb 2017
SRR4064149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA40.16129032258064516No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC40.16129032258064516No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC40.16129032258064516No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG40.16129032258064516No Hit
ACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTC30.12096774193548387No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC30.12096774193548387No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC30.12096774193548387No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA30.12096774193548387No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.12096774193548387No Hit
ATCCCTGACGCACCGCCGTGATGCCCAGGGAAGACAGGGCGACCTGGAAG30.12096774193548387No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA30.12096774193548387No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)