FastQCFastQC Report
Thu 2 Feb 2017
SRR4064148_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064148_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260650
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT36900.29270614365605047No Hit
TATCAACGCAGAGTACTTTTTTTTT27510.2182207591321937No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19500.15468210843612423No Hit
GTACATGGGGTGGTATCAACGCAAA13960.11073652480863047No Hit
GGTATCAACGCAGAGTACTTTTTTT13910.11033990401776862No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13550.10748423432356323No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13480.10692896521635664No Hit
GAATAGGACCGCGGTTCTATTTTGT13420.10645302026732241No Hit
GATTAAGAGGGACGGCCGGGGGCAT13110.1039939713639789No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12820.10169357077698014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG902.2882887E-914.7770997
GCGTTAT903.6578058E-813.7161461
GCCTTAT707.2932216E-613.5654181
CGTCTTA1153.765308E-1013.21940715
CGGTTCT2000.012.826447512
CGCGTAA853.936666E-612.29550510
TCGCGTA853.936666E-612.2955059
TAAGGGT853.9506704E-612.2916014
GTCTTAG1405.2750693E-1112.2088771
TAGACAG1501.4551915E-1112.0313475
CGAACGA802.8608776E-511.87681216
GGTTCTA2250.011.82355613
TAGGATA658.03536E-411.6899154
GCGTAAC907.4479412E-611.61242211
GTCCTAA907.4941654E-611.605971
CGGAATT1157.0527676E-811.56698115
ATCGCCA2300.011.56698116
GTATTAG2400.011.4740841
GTCCTAT2650.011.4666191
CCGTCCA752.0710478E-411.4012869
Produced by FastQC (version 0.11.5)