FastQCFastQC Report
Thu 2 Feb 2017
SRR4064148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1260650
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT13900.11026057985959624No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13170.10446991631301313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGA708.1331935E-418.8538281
TCCTATA1557.239578E-1018.448372
ATCGATT600.00740622418.3359329
CGTGCGG600.00741300618.33301710
GTATTAG3700.017.8347031
GTATCAA28550.017.7201771
GATCTAA1002.4869041E-517.5969071
ATAGTAC1002.4869041E-517.5969073
TTAACGG1154.270645E-617.21846235
GTCCTAT3700.016.6457231
CGAGCCG5100.016.39187815
TAACGGC1351.0848307E-616.2973136
TATACTG1906.184564E-1016.208325
TCCCCGC2750.015.9971891
GTATTAC1105.6300094E-515.9971881
AACGCAG31900.015.7909556
TAATACC1857.579729E-915.4573564
CGAACGT2001.2787496E-915.3979034
AACGTCT2303.6379788E-1115.3022656
GTATAAA2455.456968E-1215.2626231
Produced by FastQC (version 0.11.5)