##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064148_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1260650
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300659976995995	32.0	32.0	32.0	32.0	32.0
2	31.41026137310118	32.0	32.0	32.0	32.0	32.0
3	31.48435727600841	32.0	32.0	32.0	32.0	32.0
4	31.572704557172887	32.0	32.0	32.0	32.0	32.0
5	31.5122309919486	32.0	32.0	32.0	32.0	32.0
6	35.104296196406615	36.0	36.0	36.0	36.0	36.0
7	35.1312021576171	36.0	36.0	36.0	36.0	36.0
8	35.07659461388966	36.0	36.0	36.0	36.0	36.0
9	35.19116646174592	36.0	36.0	36.0	36.0	36.0
10	35.042582001348514	36.0	36.0	36.0	36.0	36.0
11	35.18639273390711	36.0	36.0	36.0	36.0	36.0
12	35.09115852933011	36.0	36.0	36.0	36.0	36.0
13	35.13846428429778	36.0	36.0	36.0	36.0	36.0
14	35.0766493475588	36.0	36.0	36.0	36.0	36.0
15	35.05777733708801	36.0	36.0	36.0	36.0	36.0
16	35.0631031610677	36.0	36.0	36.0	36.0	36.0
17	35.02788482132233	36.0	36.0	36.0	36.0	36.0
18	35.02614048308412	36.0	36.0	36.0	36.0	36.0
19	35.020949510173324	36.0	36.0	36.0	36.0	36.0
20	35.01694205370246	36.0	36.0	36.0	36.0	36.0
21	35.01907349383254	36.0	36.0	36.0	36.0	36.0
22	34.999366993217784	36.0	36.0	36.0	36.0	36.0
23	34.94701146234085	36.0	36.0	36.0	36.0	36.0
24	34.922175861658665	36.0	36.0	36.0	32.0	36.0
25	34.89274580573514	36.0	36.0	36.0	32.0	36.0
26	34.835870384325546	36.0	36.0	36.0	32.0	36.0
27	34.83140443422044	36.0	36.0	36.0	32.0	36.0
28	34.806772696624755	36.0	36.0	36.0	32.0	36.0
29	34.78254154602784	36.0	36.0	36.0	32.0	36.0
30	34.76792448340142	36.0	36.0	36.0	32.0	36.0
31	34.748414706698924	36.0	36.0	36.0	32.0	36.0
32	34.714738428588426	36.0	36.0	36.0	32.0	36.0
33	34.68486336413755	36.0	36.0	36.0	32.0	36.0
34	34.670145559830246	36.0	36.0	36.0	32.0	36.0
35	34.63122754134772	36.0	36.0	36.0	32.0	36.0
36	34.605245706579936	36.0	36.0	36.0	32.0	36.0
37	34.59490262959584	36.0	36.0	36.0	32.0	36.0
38	34.55464165311545	36.0	36.0	36.0	32.0	36.0
39	34.556096458176334	36.0	36.0	36.0	32.0	36.0
40	34.535593543013526	36.0	36.0	36.0	32.0	36.0
41	34.51214135564987	36.0	36.0	36.0	32.0	36.0
42	34.432691072066	36.0	36.0	36.0	32.0	36.0
43	34.42475786300718	36.0	36.0	36.0	32.0	36.0
44	34.3633768294134	36.0	36.0	36.0	32.0	36.0
45	34.3142014040376	36.0	36.0	36.0	32.0	36.0
46	34.30218220759132	36.0	36.0	36.0	32.0	36.0
47	34.25300916194027	36.0	36.0	36.0	32.0	36.0
48	34.213638995756156	36.0	36.0	36.0	32.0	36.0
49	34.185406734621026	36.0	36.0	36.0	32.0	36.0
50	33.58297782889779	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	2.0
19	4.0
20	12.0
21	44.0
22	122.0
23	373.0
24	981.0
25	2275.0
26	4569.0
27	8462.0
28	14109.0
29	21756.0
30	31190.0
31	44303.0
32	64537.0
33	104269.0
34	219400.0
35	744240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.34451348909235	17.715624372787154	11.76006733638124	26.17979480173926
2	16.22969493418454	20.22582073000668	36.64835330457559	26.896131031233196
3	18.010117027209045	24.369311948547058	28.778079827263305	28.842491196980596
4	12.016420100741682	15.907190734938325	35.77440209415778	36.30198707016222
5	14.192757704358863	37.06984492127077	33.344147860230834	15.393249514139532
6	34.53202998143017	35.723243699465435	16.612263385388616	13.132462933715782
7	30.411851029230952	30.67703169000119	20.599690635783126	18.311426644984728
8	28.698528536865904	32.58271526593424	18.991234680521956	19.727521516677903
9	27.704767835257627	13.597195064392151	18.129196793679377	40.56884030667084
10	15.690158341338584	26.84719001833209	31.643994158480954	25.81865748184838
11	37.706421290604055	20.787926863126167	22.202435251655892	19.30321659461389
12	24.732181598525838	23.62529141735725	28.80674953932459	22.835777444792324
13	29.841176414594443	19.28403260555716	25.163884490784078	25.710906489064318
14	23.853129501653118	19.259539538396126	25.359894276594257	31.5274366833565
15	25.283237691198334	26.929276647189198	22.36867782218337	25.418807839429096
16	25.90366797816982	25.50926513516944	23.581117527605024	25.00594935905572
17	24.248046642605008	25.68484511958117	25.027485820806728	25.039622417007102
18	25.051124419942095	24.76873041684845	26.115416650140798	24.064728513068655
19	25.767838629150543	24.70394217581579	25.457680924666583	24.070538270367088
20	26.011705128489883	23.904369512725243	24.956768128036146	25.127157230748733
21	27.232935390473166	23.817395787887204	24.558759370166182	24.390909451473448
22	26.148336175782333	23.82469361043906	24.986554555189784	25.04041565858882
23	24.599409823581674	23.870890976012184	25.684810889706817	25.844888310699325
24	25.0890217483562	24.5661949776583	25.313271677162604	25.031511596822902
25	25.118057456082944	24.131634162690432	25.402601111362312	25.347707269864312
26	25.10320711549995	24.984610161101468	25.444401430461728	24.46778129293686
27	25.59976835875834	24.51898744218885	24.728099193222114	25.153145005830694
28	24.880154658598443	24.359681195129554	25.655668845288254	25.104495300983753
29	24.85877767826616	24.649591742351465	25.442705503168728	25.048925076213646
30	24.725147887452433	24.797891775815554	25.654714827298715	24.822245509433305
31	25.209773372565603	24.67388639830801	24.747030408710007	25.36930982041638
32	25.058663193149854	24.66083234305315	24.655279370070744	25.62522509372625
33	24.72760533056635	24.267419971298903	25.11925075699341	25.885723941141332
34	25.492127936208004	24.402882126484513	25.254938731410594	24.85005120589689
35	25.817362877783673	24.343075450040416	25.316176546415264	24.523385125760644
36	24.96711731937532	24.942048737765184	24.90436653439552	25.18646740846398
37	25.73061374727904	24.83269659142112	24.834600470892855	24.602089190406986
38	24.991888300509707	24.640050772495588	25.193074314273815	25.17498661272089
39	25.429741635417898	24.588860868344252	24.664143495509617	25.317254000728234
40	25.689408849214946	24.82331952254823	25.06843914330296	24.41883248493387
41	24.798131681843312	25.161054387636355	25.244982742331224	24.79583118818911
42	25.767443483889597	25.212475825272445	24.906275830666644	24.113804860171314
43	25.30790453571185	24.32322876557371	24.96696064945923	25.401906049255206
44	24.8937034357588	24.927099595121145	24.73402061201437	25.445176357105687
45	25.007496275659868	25.100624610312654	24.779038350787623	25.112840763239852
46	24.89824540250081	24.988596980401084	24.722777720046548	25.390379897051556
47	25.045255868190797	24.722872260254956	25.19637318441072	25.035498687143527
48	25.91213198605763	25.313219389220208	24.175682086087935	24.598966538634226
49	24.910541400036013	25.54569547665906	24.313939200675215	25.229823922629713
50	25.13094611992547	25.56303063619626	24.31603805389832	24.989985189979954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	23.0
1	29.5
2	36.0
3	90.0
4	144.0
5	177.0
6	210.0
7	204.5
8	199.0
9	303.0
10	407.0
11	580.5
12	754.0
13	1439.0
14	2124.0
15	2956.0
16	3788.0
17	4131.0
18	4474.0
19	4554.5
20	4635.0
21	5064.5
22	5494.0
23	6156.0
24	6818.0
25	7184.5
26	7551.0
27	8801.0
28	10051.0
29	11809.0
30	13567.0
31	15697.0
32	17827.0
33	20558.5
34	23290.0
35	26647.0
36	30004.0
37	35912.5
38	41821.0
39	46290.0
40	50759.0
41	56158.0
42	61557.0
43	63952.0
44	66347.0
45	74089.0
46	81831.0
47	87575.0
48	93319.0
49	96934.5
50	100550.0
51	97849.5
52	95149.0
53	94512.0
54	93875.0
55	94614.5
56	95354.0
57	93244.5
58	91135.0
59	82676.0
60	74217.0
61	64932.5
62	55648.0
63	47920.5
64	40193.0
65	34319.0
66	28445.0
67	23981.5
68	19518.0
69	17390.5
70	15263.0
71	11772.0
72	8281.0
73	7155.0
74	6029.0
75	4748.5
76	3468.0
77	2995.5
78	2523.0
79	2099.5
80	1676.0
81	1339.0
82	1002.0
83	836.0
84	670.0
85	490.0
86	310.0
87	218.5
88	127.0
89	89.0
90	51.0
91	45.5
92	40.0
93	37.5
94	35.0
95	34.0
96	33.0
97	24.5
98	16.0
99	14.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008963629873477968
2	3.172966326894856E-4
3	2.379724745171142E-4
4	0.0
5	0.0
6	2.379724745171142E-4
7	0.0
8	0.0
9	0.0011898623725855708
10	0.0015071590052750565
11	0.0
12	7.93241581723714E-5
13	4.759449490342284E-4
14	1.586483163447428E-4
15	0.003807559592273827
16	7.93241581723714E-4
17	0.0
18	0.0
19	7.93241581723714E-5
20	4.759449490342284E-4
21	0.0
22	0.0
23	7.93241581723714E-4
24	2.379724745171142E-4
25	0.0027763455360329987
26	0.006187284337444968
27	0.006345932653789712
28	0.005314718597548884
29	0.003886883750446198
30	0.004997421964859397
31	0.00975687145520168
32	0.00507674612303177
33	0.005632015230238369
34	0.004204180383135684
35	0.0032522904850672274
36	0.00856700908261611
37	0.005235394439376512
38	0.009915519771546425
39	0.005314718597548884
40	0.0032522904850672274
41	0.004045532066790941
42	0.00285566969420537
43	0.002538373061515885
44	0.002062428112481656
45	0.0019037797961369135
46	0.0015071590052750565
47	0.003172966326894856
48	0.002538373061515885
49	8.725657398960854E-4
50	0.0013485106889303137
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1260650.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.03340361642033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.5787156123891	40.29741841878449
2	14.73032658443157	15.918593634765546
3	4.969711704079445	8.055913150913181
4	2.125012901287471	4.592867191414651
5	1.0477497474147388	2.830674249553152
6	0.6388616852425604	2.0711922776266647
7	0.3908677366285839	1.4783939921722267
8	0.2557932982576177	1.1057106021703396
9	0.18164874604996523	0.8833590010560968
>10	0.9334886031412772	8.878127030495683
>50	0.07033103755751142	2.621542060331484
>100	0.0683234149845306	7.467391147043069
>500	0.007394297206175775	2.6952904002755833
>1k	0.0017746313294821861	1.103526843397741
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1390	0.11026057985959624	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1317	0.10446991631301313	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.586483163447428E-4
2	0.0	0.0	0.0	0.0	1.586483163447428E-4
3	0.0	0.0	0.0	0.0	1.586483163447428E-4
4	0.0	0.0	0.0	0.0	1.586483163447428E-4
5	0.0	0.0	0.0	0.0	1.586483163447428E-4
6	0.0	0.0	0.0	0.0	3.172966326894856E-4
7	0.0	0.0	0.0	0.0	3.172966326894856E-4
8	0.0	0.0	0.0	0.0	3.172966326894856E-4
9	0.0	0.0	0.0	0.0	3.172966326894856E-4
10	0.0	0.0	0.0	0.0	3.172966326894856E-4
11	0.0	0.0	0.0	0.0	3.172966326894856E-4
12	0.0	0.0	0.0	0.0	7.93241581723714E-4
13	0.0	0.0	0.0	0.0	9.518898980684568E-4
14	0.0	0.0	0.0	0.0	9.518898980684568E-4
15	0.0	0.0	0.0	0.0	0.0012691865307579424
16	0.0	0.0	0.0	0.0	0.0012691865307579424
17	0.0	0.0	0.0	7.93241581723714E-5	0.0012691865307579424
18	0.0	0.0	0.0	7.93241581723714E-5	0.001427834847102685
19	0.0	0.0	0.0	7.93241581723714E-5	0.001427834847102685
20	0.0	0.0	0.0	1.586483163447428E-4	0.001586483163447428
21	0.0	0.0	0.0	2.379724745171142E-4	0.001824455637964542
22	0.0	0.0	0.0	5.552691072065998E-4	0.0019037797961369135
23	0.0	0.0	0.0	9.518898980684568E-4	0.0019037797961369135
24	0.0	0.0	0.0	0.0017451314797921706	0.0019037797961369135
25	0.0	0.0	0.0	0.0021417522706540276	0.0019037797961369135
26	0.0	0.0	0.0	0.0024590489033435134	0.002062428112481656
27	0.0	0.0	0.0	0.0029349938523777416	0.0021417522706540276
28	0.0	0.0	0.0	0.004362828699480427	0.0021417522706540276
29	0.0	0.0	0.0	0.007615119184547654	0.0021417522706540276
30	0.0	0.0	0.0	0.014040375996509737	0.0021417522706540276
31	0.0	0.0	0.0	0.029270614365605047	0.0021417522706540276
32	0.0	0.0	0.0	0.04577003926545829	0.0021417522706540276
33	0.0	0.0	0.0	0.05901717368024432	0.002221076428826399
34	0.0	0.0	0.0	0.07734105421806212	0.002221076428826399
35	0.0	0.0	0.0	0.09947249434815374	0.002221076428826399
36	0.0	0.0	0.0	0.12969499861182723	0.002221076428826399
37	0.0	0.0	0.0	0.17237139570856305	0.002221076428826399
38	0.0	0.0	0.0	0.23289572839408243	0.0023004005869987705
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACGGA	70	8.1331935E-4	18.853828	1
TCCTATA	155	7.239578E-10	18.44837	2
ATCGATT	60	0.007406224	18.33593	29
CGTGCGG	60	0.007413006	18.333017	10
GTATTAG	370	0.0	17.834703	1
GTATCAA	2855	0.0	17.720177	1
GATCTAA	100	2.4869041E-5	17.596907	1
ATAGTAC	100	2.4869041E-5	17.596907	3
TTAACGG	115	4.270645E-6	17.218462	35
GTCCTAT	370	0.0	16.645723	1
CGAGCCG	510	0.0	16.391878	15
TAACGGC	135	1.0848307E-6	16.29731	36
TATACTG	190	6.184564E-10	16.20832	5
TCCCCGC	275	0.0	15.997189	1
GTATTAC	110	5.6300094E-5	15.997188	1
AACGCAG	3190	0.0	15.790955	6
TAATACC	185	7.579729E-9	15.457356	4
CGAACGT	200	1.2787496E-9	15.397903	4
AACGTCT	230	3.6379788E-11	15.302265	6
GTATAAA	245	5.456968E-12	15.262623	1
>>END_MODULE