FastQCFastQC Report
Thu 2 Feb 2017
SRR4064147_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064147_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230240
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23550.1914260632071791No Hit
TATCAACGCAGAGTACTTTTTTTTT15390.12509754194303552No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA453.5357734E-516.89008113
ATTGCAC508.727503E-515.1967473
GCTATAT704.450685E-714.9235551
CGCATCG1850.014.37939513
GCATCGC2000.013.3014814
CATTGCG500.001498898113.3003999
CGGAATT655.4449898E-513.15531115
GTATCAA16750.012.700211
CGCCAGT2100.012.66910718
GACCATA2250.012.66704711
ACGGTAT2050.012.0491429
ATTACTG802.8679202E-511.8739065
TTGCACT802.8679202E-511.8739064
CGACCAT2250.011.822576510
CATCGCC2350.011.7247115
TGGCGAG5350.011.72188518
GGTATCA5350.011.7156881
ATCGCCA2300.011.566504516
GCATTTC4050.011.49463813
TTTAGGG1001.933282E-611.397563
Produced by FastQC (version 0.11.5)