##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064147_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1230240
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.214845883730003	32.0	32.0	32.0	32.0	32.0
2	30.756289016777213	32.0	32.0	32.0	32.0	32.0
3	30.77680046169853	32.0	32.0	32.0	32.0	32.0
4	30.80213535570295	32.0	32.0	32.0	32.0	32.0
5	30.69962365066979	32.0	32.0	32.0	32.0	32.0
6	34.36126203017297	36.0	36.0	36.0	32.0	36.0
7	34.29104321108076	36.0	36.0	36.0	32.0	36.0
8	34.253374138379506	36.0	36.0	36.0	32.0	36.0
9	34.40777815710756	36.0	36.0	36.0	32.0	36.0
10	34.06665691247236	36.0	36.0	36.0	32.0	36.0
11	34.37551453374951	36.0	36.0	36.0	32.0	36.0
12	34.18144427103655	36.0	36.0	36.0	32.0	36.0
13	34.272681753153854	36.0	36.0	36.0	32.0	36.0
14	34.15606467030823	36.0	36.0	36.0	32.0	36.0
15	34.085245968266356	36.0	36.0	36.0	32.0	36.0
16	34.100453570035114	36.0	36.0	36.0	32.0	36.0
17	34.0177989660554	36.0	36.0	36.0	32.0	36.0
18	34.01150344648199	36.0	36.0	36.0	32.0	36.0
19	34.02932273377552	36.0	36.0	36.0	32.0	36.0
20	34.013524190401874	36.0	36.0	36.0	32.0	36.0
21	33.99268760567044	36.0	36.0	36.0	32.0	36.0
22	33.962287033424374	36.0	36.0	36.0	32.0	36.0
23	33.91247236311614	36.0	36.0	36.0	32.0	36.0
24	33.89056850695799	36.0	36.0	36.0	32.0	36.0
25	33.48970607361165	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	8.0
5	15.0
6	48.0
7	24.0
8	58.0
9	60.0
10	99.0
11	37.0
12	64.0
13	73.0
14	117.0
15	191.0
16	352.0
17	487.0
18	665.0
19	942.0
20	1378.0
21	2114.0
22	3252.0
23	5008.0
24	7520.0
25	10931.0
26	15076.0
27	19863.0
28	26555.0
29	35394.0
30	46435.0
31	62486.0
32	87170.0
33	120686.0
34	254349.0
35	528778.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.155448651488136	17.26413951306065	11.432690689181573	26.14772114626965
2	16.5599752231985	19.75242707792255	37.98361707160642	25.70398062727253
3	18.726035848785138	23.978660779387152	28.776246257792288	28.519057114035423
4	12.62171831015683	15.621319187089547	36.190301228177276	35.56666127457635
5	14.538707291099314	36.762781169469896	33.80016746197556	14.898344077455228
6	33.8476571709154	35.72206676888108	17.028373441379276	13.401902618824252
7	29.970934604893557	30.439232998776404	21.036028894661317	18.55380350166872
8	27.839573931780297	33.38057486685368	19.46326787819653	19.316583323169493
9	27.61906078538685	14.06122045691302	18.365405180342638	39.95431357735749
10	15.949709288849526	26.606108959954327	31.73871211991825	25.7054696312779
11	37.0857243236913	20.845125632717856	23.01864972479213	19.05050031879871
12	25.031983948246594	23.29976747079965	29.06606871119938	22.60217986975437
13	29.708672637774143	19.568173758576823	25.100205290709216	25.62294831293982
14	23.498609145478504	19.853919606981925	25.053275421729865	31.594195825809706
15	24.885688770904192	27.791251673392768	21.998506756196228	25.324552799506804
16	25.44881638708502	25.64792884007236	24.192976405825014	24.710278367017608
17	23.920775393914845	26.059501413969876	25.333443945874677	24.6862792462406
18	24.65751418872695	24.934170977311865	26.476729837040118	23.931584996921064
19	25.789626525301646	24.704660278993646	25.17979057174256	24.32592262396215
20	26.01626280801882	24.447168650980878	24.875377041392394	24.66119149960791
21	26.574064809911587	24.16173112761307	24.358583282696777	24.905620779778566
22	26.060172211127426	24.529551250503335	24.61854462480833	24.791731913560913
23	24.373233673324947	24.492981876695143	25.42859606620644	25.705188383773468
24	25.0900101358002	24.820345788599422	25.218944672847933	24.870699402752447
25	24.916761639338127	24.659867680901584	25.245322341721028	25.17804833803926
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	66.0
1	66.0
2	189.5
3	313.0
4	313.0
5	313.0
6	795.0
7	1277.0
8	1277.0
9	1277.0
10	1550.5
11	1824.0
12	1824.0
13	1824.0
14	2416.0
15	3008.0
16	3008.0
17	3008.0
18	5097.5
19	7187.0
20	7187.0
21	7187.0
22	12055.0
23	16923.0
24	16923.0
25	16923.0
26	26256.5
27	35590.0
28	35590.0
29	35590.0
30	45684.0
31	55778.0
32	55778.0
33	55778.0
34	68378.5
35	80979.0
36	80979.0
37	80979.0
38	92141.0
39	103303.0
40	103303.0
41	103303.0
42	117624.0
43	131945.0
44	131945.0
45	131945.0
46	145725.5
47	159506.0
48	159506.0
49	159506.0
50	162659.0
51	165812.0
52	165812.0
53	165812.0
54	154144.5
55	142477.0
56	142477.0
57	142477.0
58	130630.5
59	118784.0
60	118784.0
61	118784.0
62	103805.5
63	88827.0
64	88827.0
65	88827.0
66	72435.0
67	56043.0
68	56043.0
69	56043.0
70	42620.5
71	29198.0
72	29198.0
73	29198.0
74	22219.0
75	15240.0
76	15240.0
77	15240.0
78	12149.0
79	9058.0
80	9058.0
81	9058.0
82	6199.0
83	3340.0
84	3340.0
85	3340.0
86	2621.5
87	1903.0
88	1903.0
89	1903.0
90	1355.5
91	808.0
92	808.0
93	808.0
94	583.5
95	359.0
96	359.0
97	359.0
98	525.5
99	692.0
100	692.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038772922356613344
2	0.004633242294186501
3	0.0013818441930029913
4	0.003495252958772272
5	0.008941344778254649
6	0.011867603069319808
7	0.02072766289504487
8	0.03170113148653921
9	0.04495057874886201
10	0.05437963324229419
11	0.05072181037846274
12	0.058281310963714396
13	0.06210170373260502
14	0.06340226297307842
15	0.057062036675770575
16	0.06372740278319677
17	0.05876902067889192
18	0.07470087137469111
19	0.07258746260892183
20	0.07575757575757576
21	0.07242489270386265
22	0.07600143061516453
23	0.0807972428144102
24	0.07592014566263493
25	0.07632657042528287
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1230240.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.1512059153794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.89093927122394	47.039056662493934
2	11.473445550591444	13.343893895426731
3	3.3135608931040617	5.780626854241283
4	1.3772838741786029	3.2036287268516572
5	0.7502220431241112	2.181315825598342
6	0.48330344146753124	1.686280676663395
7	0.31900372749223527	1.2985316011622183
8	0.21801401604764797	1.0142222351700485
9	0.16203751141434417	0.8480409023044051
>10	0.8638250796611622	8.88924040844924
>50	0.0689568033864078	2.7665290890650875
>100	0.07197208458107109	8.513131370533346
>500	0.006593925947049317	2.769613238619445
>1k	8.417777804743809E-4	0.6658885134208764
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2355	0.1914260632071791	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1539	0.12509754194303552	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4385485758876316E-4
2	0.0	0.0	0.0	0.0	2.4385485758876316E-4
3	0.0	0.0	0.0	0.0	2.4385485758876316E-4
4	0.0	0.0	0.0	0.0	2.4385485758876316E-4
5	0.0	0.0	0.0	0.0	4.064247626479386E-4
6	0.0	0.0	0.0	0.0	4.064247626479386E-4
7	0.0	0.0	0.0	0.0	4.064247626479386E-4
8	0.0	0.0	0.0	0.0	4.064247626479386E-4
9	0.0	0.0	0.0	0.0	4.877097151775263E-4
10	0.0	0.0	0.0	0.0	4.877097151775263E-4
11	0.0	0.0	0.0	0.0	4.877097151775263E-4
12	0.0	0.0	0.0	0.0	8.94134477825465E-4
13	0.0	0.0	0.0	0.0	0.0010567043828846403
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGTA	45	3.5357734E-5	16.890081	13
ATTGCAC	50	8.727503E-5	15.196747	3
GCTATAT	70	4.450685E-7	14.923555	1
CGCATCG	185	0.0	14.379395	13
GCATCGC	200	0.0	13.30148	14
CATTGCG	50	0.0014988981	13.300399	9
CGGAATT	65	5.4449898E-5	13.155311	15
GTATCAA	1675	0.0	12.70021	1
CGCCAGT	210	0.0	12.669107	18
GACCATA	225	0.0	12.667047	11
ACGGTAT	205	0.0	12.049142	9
ATTACTG	80	2.8679202E-5	11.873906	5
TTGCACT	80	2.8679202E-5	11.873906	4
CGACCAT	225	0.0	11.8225765	10
CATCGCC	235	0.0	11.72471	15
TGGCGAG	535	0.0	11.721885	18
GGTATCA	535	0.0	11.715688	1
ATCGCCA	230	0.0	11.5665045	16
GCATTTC	405	0.0	11.494638	13
TTTAGGG	100	1.933282E-6	11.39756	3
>>END_MODULE