FastQCFastQC Report
Thu 2 Feb 2017
SRR4064147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1230240
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA15050.024.4134271
GGTATCA6800.020.7071251
GTATTGA1252.2864697E-819.3611621
TAGACCG708.126817E-418.8559765
GTATTAC1303.5850462E-818.6165031
CGCAATA2850.018.52742836
TATTACC600.00741656118.3314532
GTATAGA1601.1004886E-917.8760741
GTGTATA1852.1827873E-1117.8389071
GTCCTAT5000.017.6010571
GATCGGT1002.4843926E-517.598916
ATTATAC1509.607902E-917.5981963
TAGGACA3900.017.4860974
GGCGTTA3300.017.33296442
ATACGAA3050.017.31110640
TATAAGG1053.777751E-516.7601852
TAGGACG5400.016.702674
GTCCTAC6550.016.4590031
CGACGGT3750.016.4256527
TGTAGGA8050.016.3958342
Produced by FastQC (version 0.11.5)