##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1230240
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35358385355703	32.0	32.0	32.0	32.0	32.0
2	31.404254454415398	32.0	32.0	32.0	32.0	32.0
3	31.474814670308234	32.0	32.0	32.0	32.0	32.0
4	31.56083284562362	32.0	32.0	32.0	32.0	32.0
5	31.516262680452595	32.0	32.0	32.0	32.0	32.0
6	35.09269329561712	36.0	36.0	36.0	36.0	36.0
7	35.10957049031084	36.0	36.0	36.0	36.0	36.0
8	35.06450042268175	36.0	36.0	36.0	36.0	36.0
9	35.166480524125376	36.0	36.0	36.0	36.0	36.0
10	35.02436028742359	36.0	36.0	36.0	36.0	36.0
11	35.16640899336715	36.0	36.0	36.0	36.0	36.0
12	35.073256437768244	36.0	36.0	36.0	36.0	36.0
13	35.11508973858759	36.0	36.0	36.0	36.0	36.0
14	35.05695392768891	36.0	36.0	36.0	36.0	36.0
15	35.0226638704643	36.0	36.0	36.0	36.0	36.0
16	35.04280790740018	36.0	36.0	36.0	36.0	36.0
17	35.0030912667447	36.0	36.0	36.0	36.0	36.0
18	35.004173982312395	36.0	36.0	36.0	36.0	36.0
19	34.99302249967486	36.0	36.0	36.0	36.0	36.0
20	34.991908895825205	36.0	36.0	36.0	36.0	36.0
21	34.98405758226037	36.0	36.0	36.0	36.0	36.0
22	34.97123325529978	36.0	36.0	36.0	36.0	36.0
23	34.92329951879308	36.0	36.0	36.0	32.0	36.0
24	34.89401824034335	36.0	36.0	36.0	32.0	36.0
25	34.861750552737675	36.0	36.0	36.0	32.0	36.0
26	34.80605247756535	36.0	36.0	36.0	32.0	36.0
27	34.799509851736246	36.0	36.0	36.0	32.0	36.0
28	34.77136412407335	36.0	36.0	36.0	32.0	36.0
29	34.7446945311484	36.0	36.0	36.0	32.0	36.0
30	34.72318084276239	36.0	36.0	36.0	32.0	36.0
31	34.71808671478736	36.0	36.0	36.0	32.0	36.0
32	34.691423624658604	36.0	36.0	36.0	32.0	36.0
33	34.672573644166995	36.0	36.0	36.0	32.0	36.0
34	34.65810979971388	36.0	36.0	36.0	32.0	36.0
35	34.627637696709584	36.0	36.0	36.0	32.0	36.0
36	34.591295194433606	36.0	36.0	36.0	32.0	36.0
37	34.58883226037196	36.0	36.0	36.0	32.0	36.0
38	34.55016582130316	36.0	36.0	36.0	32.0	36.0
39	34.5369635193133	36.0	36.0	36.0	32.0	36.0
40	34.53016484588373	36.0	36.0	36.0	32.0	36.0
41	34.5083032579009	36.0	36.0	36.0	32.0	36.0
42	34.45298722200546	36.0	36.0	36.0	32.0	36.0
43	34.43970688646118	36.0	36.0	36.0	32.0	36.0
44	34.37459276238783	36.0	36.0	36.0	32.0	36.0
45	34.35691491091169	36.0	36.0	36.0	32.0	36.0
46	34.32091624398491	36.0	36.0	36.0	32.0	36.0
47	34.30269540902588	36.0	36.0	36.0	32.0	36.0
48	34.2613977760437	36.0	36.0	36.0	32.0	36.0
49	34.243069644947326	36.0	36.0	36.0	32.0	36.0
50	33.657529425152816	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	0.0
17	1.0
18	2.0
19	3.0
20	10.0
21	41.0
22	122.0
23	360.0
24	972.0
25	2356.0
26	4585.0
27	8555.0
28	14044.0
29	21214.0
30	31090.0
31	43874.0
32	63123.0
33	101588.0
34	212828.0
35	725471.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.330631741616195	18.193309861731073	11.925400300291269	26.550658096361467
2	15.823249885794086	20.44806110057111	37.4451527107851	26.283536302849704
3	18.031676874743443	24.638707238860867	29.05298581165387	28.276630074741817
4	12.285570295226949	16.21073936792821	36.337869033684484	35.165821303160364
5	14.070100143061518	37.16827610872675	33.64782481467031	15.113798933541423
6	33.92787905439512	36.04055797288169	16.821203034532314	13.210359938190871
7	29.92166562757318	30.597713127286646	21.0444474610218	18.43617378411837
8	27.79571465730264	33.58629210560541	19.324765899336715	19.293227337755233
9	27.66970403908212	13.910569568292107	18.301211968493696	40.11851442413207
10	15.794568996279567	26.809718159080436	31.873311803536403	25.5224010411036
11	37.2208674730134	20.805696449473274	22.962186240083234	19.011249837430093
12	24.94216566225397	23.42302871476901	29.10233629590372	22.532469327073297
13	29.75978553673529	19.537014909358707	25.18650923320279	25.516690320703216
14	23.530688420758295	19.75889178986796	25.082504495072488	31.627915294301257
15	24.941390500221104	27.880400853211246	21.963930364435658	25.214278282131986
16	25.510737414355518	25.69037847363625	24.11547099691034	24.683413115097895
17	23.938012095200936	26.171234880998828	25.183622707764336	24.707130316035897
18	24.772218686318165	24.85756809460291	26.42889134370044	23.941321875378485
19	25.8535732865132	24.697050981922228	25.211909871244636	24.237465860319936
20	26.09922356108441	24.392553599646245	24.883843047490227	24.62437979177911
21	26.72876837039927	24.123504356873458	24.27266224476525	24.875065027962023
22	26.04049310944097	24.535413587984074	24.735863258534554	24.688230044040402
23	24.439984588297836	24.37544402121873	25.558552275420777	25.626019115062665
24	25.28563625190614	24.684938499604954	25.15631147194522	24.873113776543686
25	24.938851853628126	24.592000221100644	25.390319856219552	25.078828069051685
26	24.589245766459435	25.34840543141794	25.671123481518094	24.39122532060453
27	25.16629828909686	24.94795430546535	24.815370896193937	25.070376509243854
28	24.50934328736746	24.87814870572628	25.405303904769028	25.207204102137233
29	24.845047158500194	24.73043286000067	25.181574247711573	25.242945733787565
30	24.563159041323495	25.12266338046945	25.460983453043255	24.853194125163792
31	25.44783229335007	24.50737634821176	24.71694462780088	25.327846730637287
32	24.937875803631453	25.008758759775873	24.568504068860516	25.484861367732158
33	24.481604046207533	24.691084052938205	25.41715134828876	25.410160552565504
34	25.29096847509596	24.643188679582703	25.43769234646017	24.628150498861164
35	25.58541056165436	24.70531334208526	25.287086298625027	24.422189797635355
36	24.599253907879003	25.090578900176645	25.15203516989488	25.158132022049468
37	25.477790883219136	24.848214873601133	24.647839810501278	25.02615443267845
38	24.879466408704594	24.985713182485135	25.12593941413887	25.0088809946714
39	25.627419325994676	24.586692548679157	24.6140865129028	25.171801612423366
40	25.88680269808471	24.65912213076509	25.15822553295952	24.295849638190678
41	24.905035534959037	24.90219046217801	25.507540662186624	24.68523334067633
42	25.75734175239631	25.306854686626174	24.923428578860435	24.012374982117077
43	24.8993878325729	24.551482297054427	25.380846824833625	25.16828304553905
44	24.683268548278278	25.062387113870503	24.873478120209196	25.380866217642016
45	24.787599656647895	25.211424911235675	24.939116778732977	25.06185865338345
46	24.763885806811892	24.66447358260359	25.11495808510759	25.45668252547692
47	25.358859453250933	24.5948479008175	25.155643272690913	24.890649373240656
48	25.543357215201812	25.29600348554976	24.352515237087292	24.808124062161138
49	24.99489934849846	25.22761911342142	24.59432547440123	25.18315606367889
50	24.92401808771877	25.701760731962477	24.444756943230804	24.929464237087952
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	21.5
2	28.0
3	66.5
4	105.0
5	127.0
6	149.0
7	149.0
8	149.0
9	178.5
10	208.0
11	353.0
12	498.0
13	901.5
14	1305.0
15	1833.0
16	2361.0
17	2460.5
18	2560.0
19	2545.5
20	2531.0
21	2884.0
22	3237.0
23	3780.0
24	4323.0
25	5479.0
26	6635.0
27	8727.5
28	10820.0
29	13055.5
30	15291.0
31	17819.0
32	20347.0
33	24315.5
34	28284.0
35	33542.0
36	38800.0
37	44027.0
38	49254.0
39	52569.5
40	55885.0
41	59746.5
42	63608.0
43	65587.5
44	67567.0
45	73594.5
46	79622.0
47	84685.0
48	89748.0
49	92562.0
50	95376.0
51	93328.0
52	91280.0
53	89992.5
54	88705.0
55	88247.5
56	87790.0
57	84927.5
58	82065.0
59	74929.5
60	67794.0
61	59608.0
62	51422.0
63	44690.5
64	37959.0
65	31905.0
66	25851.0
67	22460.0
68	19069.0
69	17153.5
70	15238.0
71	11887.0
72	8536.0
73	7273.5
74	6011.0
75	4727.5
76	3444.0
77	2986.0
78	2528.0
79	2083.0
80	1638.0
81	1305.0
82	972.0
83	789.5
84	607.0
85	467.0
86	327.0
87	219.0
88	111.0
89	86.5
90	62.0
91	50.5
92	39.0
93	34.0
94	29.0
95	27.0
96	25.0
97	19.5
98	14.0
99	16.0
100	18.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00820978020548836
2	4.877097151775264E-4
3	4.064247626479386E-4
4	0.0
5	0.0
6	8.128495252958772E-5
7	8.128495252958772E-5
8	0.0
9	8.128495252958773E-4
10	5.689946677071141E-4
11	0.0
12	1.6256990505917544E-4
13	4.877097151775264E-4
14	3.251398101183509E-4
15	0.005202236961893614
16	7.315645727662895E-4
17	0.0
18	2.438548575887632E-4
19	0.0
20	6.502796202367018E-4
21	0.0
22	4.877097151775264E-4
23	4.877097151775264E-4
24	3.251398101183509E-4
25	0.0025198335284172192
26	0.005202236961893614
27	0.005771231629600728
28	0.004226817531538562
29	0.0021946937182988685
30	0.0035765379113018602
31	0.00747821563272207
32	0.003495252958772272
33	0.004145532579008974
34	0.0030888281961243334
35	0.00284497333853557
36	0.00788464039537001
37	0.003820392768890623
38	0.006909220965014956
39	0.003413968006242684
40	0.0027636883860059826
41	0.003495252958772272
42	0.0013005592404734035
43	0.001706984003121342
44	0.001463129145532579
45	0.0013005592404734035
46	7.315645727662895E-4
47	0.0018695539081805175
48	0.001463129145532579
49	4.064247626479386E-4
50	0.0010567043828846405
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1230240.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.73784631685672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.85839339154197	50.537609109518165
2	11.23582788416792	13.873516303108246
3	3.0286049691464605	5.609386444188982
4	1.2798516072983257	3.1606112735906438
5	0.6599059864320805	2.037058718695876
6	0.4373302100204072	1.619989517757514
7	0.28281563404765653	1.222229970558703
8	0.21413733676012048	1.057630239007785
9	0.1493283949748574	0.8297292149730563
>10	0.7351157725933813	8.007830745195832
>50	0.055337091698299125	2.3783562365857196
>100	0.05778526165821701	7.144642504283016
>500	0.005036320645157182	2.161056374554861
>1k	5.301390152797034E-4	0.36035334798150803
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.128495252958772E-5
2	0.0	0.0	0.0	0.0	8.128495252958772E-5
3	0.0	0.0	0.0	0.0	8.128495252958772E-5
4	0.0	8.128495252958772E-5	0.0	0.0	8.128495252958772E-5
5	0.0	8.128495252958772E-5	0.0	0.0	8.128495252958772E-5
6	0.0	8.128495252958772E-5	0.0	0.0	8.128495252958772E-5
7	0.0	8.128495252958772E-5	0.0	0.0	1.6256990505917544E-4
8	0.0	8.128495252958772E-5	0.0	0.0	1.6256990505917544E-4
9	0.0	8.128495252958772E-5	0.0	0.0	1.6256990505917544E-4
10	0.0	8.128495252958772E-5	0.0	0.0	2.4385485758876316E-4
11	0.0	8.128495252958772E-5	0.0	0.0	3.251398101183509E-4
12	0.0	8.128495252958772E-5	0.0	0.0	7.315645727662895E-4
13	0.0	8.128495252958772E-5	0.0	0.0	8.94134477825465E-4
14	0.0	8.128495252958772E-5	0.0	0.0	9.754194303550526E-4
15	0.0	8.128495252958772E-5	0.0	0.0	0.001219274287943816
16	0.0	8.128495252958772E-5	0.0	0.0	0.001219274287943816
17	0.0	8.128495252958772E-5	0.0	1.6256990505917544E-4	0.0013005592404734035
18	0.0	8.128495252958772E-5	0.0	1.6256990505917544E-4	0.0013005592404734035
19	0.0	8.128495252958772E-5	0.0	1.6256990505917544E-4	0.0013005592404734035
20	0.0	8.128495252958772E-5	0.0	1.6256990505917544E-4	0.0015444140980621667
21	0.0	8.128495252958772E-5	0.0	1.6256990505917544E-4	0.0015444140980621667
22	0.0	8.128495252958772E-5	0.0	1.6256990505917544E-4	0.0016256990505917545
23	0.0	8.128495252958772E-5	0.0	3.251398101183509E-4	0.0016256990505917545
24	0.0	8.128495252958772E-5	0.0	8.94134477825465E-4	0.0016256990505917545
25	0.0	8.128495252958772E-5	0.0	0.0010567043828846403	0.0016256990505917545
26	0.0	8.128495252958772E-5	0.0	0.0010567043828846403	0.001706984003121342
27	0.0	8.128495252958772E-5	0.0	0.0013005592404734035	0.001706984003121342
28	0.0	8.128495252958772E-5	0.0	0.0021946937182988685	0.001706984003121342
29	0.0	8.128495252958772E-5	0.0	0.004145532579008974	0.001706984003121342
30	0.0	8.128495252958772E-5	0.0	0.008372350110547535	0.001706984003121342
31	0.0	8.128495252958772E-5	0.0	0.020158668227337754	0.001706984003121342
32	0.0	8.128495252958772E-5	0.0	0.0324326960593055	0.001706984003121342
33	0.0	8.128495252958772E-5	0.0	0.04519443360645077	0.001706984003121342
34	0.0	8.128495252958772E-5	0.0	0.05820002601118481	0.001706984003121342
35	0.0	8.128495252958772E-5	0.0	0.07535115099492781	0.001706984003121342
36	0.0	8.128495252958772E-5	0.0	0.09900507218103785	0.001706984003121342
37	0.0	8.128495252958772E-5	0.0	0.13485173624658603	0.001706984003121342
38	0.0	8.128495252958772E-5	0.0	0.18557354662504877	0.001706984003121342
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1505	0.0	24.413427	1
GGTATCA	680	0.0	20.707125	1
GTATTGA	125	2.2864697E-8	19.361162	1
TAGACCG	70	8.126817E-4	18.855976	5
GTATTAC	130	3.5850462E-8	18.616503	1
CGCAATA	285	0.0	18.527428	36
TATTACC	60	0.007416561	18.331453	2
GTATAGA	160	1.1004886E-9	17.876074	1
GTGTATA	185	2.1827873E-11	17.838907	1
GTCCTAT	500	0.0	17.601057	1
GATCGGT	100	2.4843926E-5	17.59891	6
ATTATAC	150	9.607902E-9	17.598196	3
TAGGACA	390	0.0	17.486097	4
GGCGTTA	330	0.0	17.332964	42
ATACGAA	305	0.0	17.311106	40
TATAAGG	105	3.777751E-5	16.760185	2
TAGGACG	540	0.0	16.70267	4
GTCCTAC	655	0.0	16.459003	1
CGACGGT	375	0.0	16.425652	7
TGTAGGA	805	0.0	16.395834	2
>>END_MODULE