FastQCFastQC Report
Thu 2 Feb 2017
SRR4064146_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064146_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805447
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29810.3701050472594721No Hit
TATCAACGCAGAGTACTTTTTTTTT22160.2751267308711809No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18650.2315484445283178No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG12240.15196530622126597No Hit
GAATAGGACCGCGGTTCTATTTTGT12170.15109622358764763No Hit
GTACATGGGGTGGTATCAACGCAAA12070.14985467696819282No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11820.14675081041955584No Hit
CTATTGGAGCTGGAATTACCGCGGC11750.1458817277859375No Hit
GATTAAGAGGGACGGCCGGGGGCAT11120.13805998408337233No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10990.13644597347808113No Hit
GGTATCAACGCAGAGTACTTTTTTT10880.13508027219668084No Hit
GTCCTATTCCATTATTCCTAGCTGC10730.13321795226749866No Hit
GTATCTGATCGTCTTCGAACCTCCG10630.1319764056480439No Hit
GTACATGGGGAATAATTGCAATCCC10190.12651360052244282No Hit
GAACTACGACGGTATCTGATCGTCT9840.12216818735435106No Hit
GAATAACGCCGCCGCATCGCCAGTC9840.12216818735435106No Hit
ATCAGATACCGTCGTAGTTCCGACC9790.12154741404462367No Hit
GCGCAAGACGGACCAGAGCGAAAGC9730.12080248607295078No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9620.11943678479155054No Hit
CTTTAATATACGCTATTGGAGCTGG9460.11745031020042287No Hit
TCGTAGTTCCGACCATAAACGATGC9090.11285658770844016No Hit
GCTTTGAACACTCTAATTTTTTCAA9040.11223581439871277No Hit
CTCTTAATCATGGCCTCAGTTCCGA9000.11173919575093086No Hit
ATATTAAAGTTGCTGCAGTTAAAAA8870.11012518514563963No Hit
GAATAATGGAATAGGACCGCGGTTC8480.10528315332976594No Hit
GGGTAGGCACACGCTGAGCCAGTCA8210.10193097745723803No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT250.006016184619.00733217
GGTAGCG250.00603564718.9967087
AGCCGCT350.002166988816.2879512
CGACCAT1650.014.96896310
GTATTAA400.00528224814.2475311
TTATGCA400.00528442514.2466464
CGGTCCA1550.013.48321110
GACCGAG500.00150041113.2976957
AGCCCCG1950.013.15810416
GTTATAT655.4549848E-513.1515671
GACCATA1900.013.0001711
ACGGTAT1900.012.9985549
TCGGCGT1051.9841536E-812.668406513
GTATAGA604.0941988E-412.6644731
CGTTATT1155.34601E-912.385312
AGCTCGT1001.4423131E-712.347868
CGTCTTA701.0863525E-412.21824115
CGAACGA1251.3988029E-912.16393716
ACGAACG1251.3988029E-912.16393715
GTGTAAG550.00307021912.0888151
Produced by FastQC (version 0.11.5)