##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064146_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	805447
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.231989193578226	32.0	32.0	32.0	32.0	32.0
2	30.80655958740923	32.0	32.0	32.0	32.0	32.0
3	30.82336392090355	32.0	32.0	32.0	32.0	32.0
4	30.84910863160456	32.0	32.0	32.0	32.0	32.0
5	30.747314224275463	32.0	32.0	32.0	32.0	32.0
6	34.41626450902418	36.0	36.0	36.0	32.0	36.0
7	34.32747778562711	36.0	36.0	36.0	32.0	36.0
8	34.27500878394233	36.0	36.0	36.0	32.0	36.0
9	34.42544326318181	36.0	36.0	36.0	32.0	36.0
10	34.097028109857014	36.0	36.0	36.0	32.0	36.0
11	34.42685986787461	36.0	36.0	36.0	32.0	36.0
12	34.201747601021545	36.0	36.0	36.0	32.0	36.0
13	34.315067285619044	36.0	36.0	36.0	32.0	36.0
14	34.18975426067761	36.0	36.0	36.0	32.0	36.0
15	34.11276595480522	36.0	36.0	36.0	32.0	36.0
16	34.11628946411123	36.0	36.0	36.0	32.0	36.0
17	34.02210697910601	36.0	36.0	36.0	32.0	36.0
18	34.02164884840343	36.0	36.0	36.0	32.0	36.0
19	34.04815959336865	36.0	36.0	36.0	32.0	36.0
20	34.02666221365279	36.0	36.0	36.0	32.0	36.0
21	33.99134766160902	36.0	36.0	36.0	32.0	36.0
22	33.98623248953687	36.0	36.0	36.0	32.0	36.0
23	33.94156784990198	36.0	36.0	36.0	32.0	36.0
24	33.9134195049457	36.0	36.0	36.0	32.0	36.0
25	33.515723567162084	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	5.0
5	11.0
6	38.0
7	10.0
8	49.0
9	61.0
10	61.0
11	25.0
12	47.0
13	52.0
14	98.0
15	157.0
16	279.0
17	339.0
18	396.0
19	602.0
20	877.0
21	1342.0
22	2007.0
23	3173.0
24	4645.0
25	6805.0
26	9438.0
27	12228.0
28	16789.0
29	22721.0
30	29801.0
31	40413.0
32	57460.0
33	80481.0
34	169369.0
35	345664.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.64617155973216	17.354577397875346	11.516806832281935	25.482444210110554
2	16.7814975905993	20.20146836241459	37.04008135219466	25.97695269479145
3	18.567776587251824	24.643638887405906	28.763286331699845	28.025298193642428
4	12.38716982818774	15.443858686340192	35.96361622718254	36.20535525828952
5	14.692698723313374	36.71746758616579	32.97345554691904	15.616378143601791
6	33.74400874164949	35.81356942409417	17.07576924032086	13.36665259393548
7	30.106686966704878	30.593490493050012	20.981480354621468	18.31834218562365
8	28.72564775784252	31.554271065946978	19.6662286595741	20.053852516636404
9	27.912968083494192	13.644697517384271	18.722346025901885	39.71998837321965
10	16.539187320722466	25.991376043986897	30.707842681749685	26.761593953540952
11	37.33835524207273	20.912142269747058	22.600576890110137	19.148925598070075
12	25.261983533705962	23.675236080241813	28.39895293552006	22.663827450532168
13	29.450877105799332	19.01803905978234	25.588381454057547	25.94270238036078
14	24.146820540662763	19.756094227737325	25.08395442420869	31.01313080739122
15	25.374345136712222	26.847872944409453	22.51301030264405	25.26477161623427
16	25.946020489552712	25.203690405492374	23.742387234158862	25.107901870796052
17	23.94500758468949	25.411256648250554	25.224155217160654	25.419580549899308
18	24.98536923575846	24.30968264598769	26.37401824776252	24.33092987049133
19	25.842988401493454	24.172604663009647	25.541066913505084	24.443340021991812
20	26.271777176661505	23.575451758762124	24.71399691102996	25.43877415354641
21	27.990333422378498	23.510533214882553	24.11450794262178	24.384625420117167
22	26.410318854697586	23.512645094915868	24.933587469465145	25.1434485809214
23	24.728867306516626	23.254660947448468	25.614459747605533	26.40201199842937
24	25.10968495474059	24.547312703077143	25.303147967520083	25.039854374662184
25	25.17768566287935	23.968429109633295	25.326419745723115	25.52746548176423
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	304.0
1	304.0
2	427.0
3	550.0
4	550.0
5	550.0
6	1456.0
7	2362.0
8	2362.0
9	2362.0
10	2231.0
11	2100.0
12	2100.0
13	2100.0
14	1978.5
15	1857.0
16	1857.0
17	1857.0
18	3219.5
19	4582.0
20	4582.0
21	4582.0
22	7994.5
23	11407.0
24	11407.0
25	11407.0
26	17271.5
27	23136.0
28	23136.0
29	23136.0
30	28476.0
31	33816.0
32	33816.0
33	33816.0
34	41415.0
35	49014.0
36	49014.0
37	49014.0
38	55229.0
39	61444.0
40	61444.0
41	61444.0
42	71303.0
43	81162.0
44	81162.0
45	81162.0
46	91919.0
47	102676.0
48	102676.0
49	102676.0
50	106268.5
51	109861.0
52	109861.0
53	109861.0
54	101093.5
55	92326.0
56	92326.0
57	92326.0
58	86212.5
59	80099.0
60	80099.0
61	80099.0
62	71033.0
63	61967.0
64	61967.0
65	61967.0
66	51579.0
67	41191.0
68	41191.0
69	41191.0
70	31179.0
71	21167.0
72	21167.0
73	21167.0
74	16290.0
75	11413.0
76	11413.0
77	11413.0
78	9399.5
79	7386.0
80	7386.0
81	7386.0
82	5037.5
83	2689.0
84	2689.0
85	2689.0
86	2102.5
87	1516.0
88	1516.0
89	1516.0
90	1105.5
91	695.0
92	695.0
93	695.0
94	475.5
95	256.0
96	256.0
97	256.0
98	363.5
99	471.0
100	471.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040722729118117015
2	0.007449279716728724
3	0.001738165267236702
4	0.004097103844200797
5	0.010056527617583777
6	0.013284548828166223
7	0.02408600441742287
8	0.035384078654461436
9	0.0507792567357008
10	0.05959423773382979
11	0.05475220591795611
12	0.06580197083110373
13	0.06542950684526729
14	0.06778844542223139
15	0.06307056826830319
16	0.06816090940806782
17	0.06654689880277659
18	0.07896236499732447
19	0.0747411064911782
20	0.08045222094067021
21	0.07598265311063299
22	0.0780932823637061
23	0.08380439681319814
24	0.07970729296899734
25	0.08132130357428857
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	805447.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.68383556125252
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35860522249493	35.93387339554794
2	14.269413901849823	13.608407721025154
3	4.615032207869218	6.601873107297599
4	2.0192656310899006	3.851453212295185
5	1.0065240611409594	2.3997463909944794
6	0.5947872846924993	1.7017043446320619
7	0.4017809219690288	1.3410918790373723
8	0.2511402544647405	0.958026447736624
9	0.19050688367275476	0.817568902290447
>10	1.0351114873395986	9.09085729060349
>50	0.10836817926572911	3.6609959262996457
>100	0.13244967751383582	13.135675445743106
>500	0.013349671053593292	4.462185454031314
>1k	0.003664615583339335	2.436540482465602
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2981	0.3701050472594721	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2216	0.2751267308711809	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1865	0.2315484445283178	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1224	0.15196530622126597	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1217	0.15109622358764763	No Hit
GTACATGGGGTGGTATCAACGCAAA	1207	0.14985467696819282	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1182	0.14675081041955584	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1175	0.1458817277859375	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1112	0.13805998408337233	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1099	0.13644597347808113	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1088	0.13508027219668084	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1073	0.13321795226749866	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1063	0.1319764056480439	No Hit
GTACATGGGGAATAATTGCAATCCC	1019	0.12651360052244282	No Hit
GAACTACGACGGTATCTGATCGTCT	984	0.12216818735435106	No Hit
GAATAACGCCGCCGCATCGCCAGTC	984	0.12216818735435106	No Hit
ATCAGATACCGTCGTAGTTCCGACC	979	0.12154741404462367	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	973	0.12080248607295078	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	962	0.11943678479155054	No Hit
CTTTAATATACGCTATTGGAGCTGG	946	0.11745031020042287	No Hit
TCGTAGTTCCGACCATAAACGATGC	909	0.11285658770844016	No Hit
GCTTTGAACACTCTAATTTTTTCAA	904	0.11223581439871277	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	900	0.11173919575093086	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	887	0.11012518514563963	No Hit
GAATAATGGAATAGGACCGCGGTTC	848	0.10528315332976594	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	821	0.10193097745723803	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2415466194547873E-4
2	0.0	0.0	0.0	0.0	1.2415466194547873E-4
3	0.0	0.0	0.0	0.0	1.2415466194547873E-4
4	0.0	0.0	0.0	0.0	1.2415466194547873E-4
5	0.0	0.0	0.0	1.2415466194547873E-4	1.2415466194547873E-4
6	0.0	0.0	0.0	1.2415466194547873E-4	1.2415466194547873E-4
7	0.0	0.0	0.0	1.2415466194547873E-4	1.2415466194547873E-4
8	0.0	0.0	0.0	1.2415466194547873E-4	1.2415466194547873E-4
9	0.0	0.0	0.0	1.2415466194547873E-4	1.2415466194547873E-4
10	0.0	0.0	0.0	1.2415466194547873E-4	1.2415466194547873E-4
11	0.0	0.0	0.0	1.2415466194547873E-4	1.2415466194547873E-4
12	0.0	0.0	0.0	1.2415466194547873E-4	1.2415466194547873E-4
13	0.0	0.0	0.0	1.2415466194547873E-4	1.2415466194547873E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACAAT	25	0.0060161846	19.007332	17
GGTAGCG	25	0.006035647	18.996708	7
AGCCGCT	35	0.0021669888	16.28795	12
CGACCAT	165	0.0	14.968963	10
GTATTAA	40	0.005282248	14.247531	1
TTATGCA	40	0.005284425	14.246646	4
CGGTCCA	155	0.0	13.483211	10
GACCGAG	50	0.001500411	13.297695	7
AGCCCCG	195	0.0	13.158104	16
GTTATAT	65	5.4549848E-5	13.151567	1
GACCATA	190	0.0	13.00017	11
ACGGTAT	190	0.0	12.998554	9
TCGGCGT	105	1.9841536E-8	12.6684065	13
GTATAGA	60	4.0941988E-4	12.664473	1
CGTTATT	115	5.34601E-9	12.38531	2
AGCTCGT	100	1.4423131E-7	12.34786	8
CGTCTTA	70	1.0863525E-4	12.218241	15
CGAACGA	125	1.3988029E-9	12.163937	16
ACGAACG	125	1.3988029E-9	12.163937	15
GTGTAAG	55	0.003070219	12.088815	1
>>END_MODULE