FastQCFastQC Report
Thu 2 Feb 2017
SRR4064146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences805447
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12410.1540759354743391No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12170.15109622358764763No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA10970.13619766415419016No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG10660.13234886963388032No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10620.1318522509860984No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG10280.12763099247995213No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT10170.12626529119855184No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC9970.12378219795964229No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA9750.12105079539684174No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9480.11769861952431383No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA9390.11658122756680453No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT9190.11409813432789494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9080.11273243304649468No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC8990.11161504108898536No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA8850.10987687582174865No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG8490.10540730799171144No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC8410.1044140706961476No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT8370.1039174520483657No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA8170.10143435880945612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCCG500.002580161721.99931732
TAGGACG1351.1277734E-1021.1845264
ACGTATG654.9291656E-420.30832322
AACGTAT654.9312296E-420.30706221
CAACGTA654.9312296E-420.30706220
TGCTACG550.00447991920.00186310
GTTATAT1001.1221491E-619.8006151
CTCGTTT901.0010106E-519.55616227
GTATATA808.981682E-519.2505991
CGTCGTA2750.019.20178810
CCCTCGT708.118416E-418.85772725
ATACCGT2700.018.7401586
GTATCAA21100.018.6640851
CCGTCGT2600.018.6171199
GTCGTAG2600.018.61711911
TAATACT600.00741133718.3327644
CGTTTAG600.00741133718.33276429
GGACGTG1601.1004886E-917.8744456
GTATAAT750.001290094617.6005461
GTCGATA750.001290631717.59945336
Produced by FastQC (version 0.11.5)