##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064146_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	805447
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.290103507741662	32.0	32.0	32.0	32.0	32.0
2	31.436844385788262	32.0	32.0	32.0	32.0	32.0
3	31.50557268200142	32.0	32.0	32.0	32.0	32.0
4	31.583793843666932	32.0	32.0	32.0	32.0	32.0
5	31.53547781542423	32.0	32.0	32.0	32.0	32.0
6	35.12932322052227	36.0	36.0	36.0	36.0	36.0
7	35.14055673433509	36.0	36.0	36.0	36.0	36.0
8	35.104525809891896	36.0	36.0	36.0	36.0	36.0
9	35.19572982455705	36.0	36.0	36.0	36.0	36.0
10	35.05730606731417	36.0	36.0	36.0	36.0	36.0
11	35.199304237274454	36.0	36.0	36.0	36.0	36.0
12	35.09551218143466	36.0	36.0	36.0	36.0	36.0
13	35.1370853699871	36.0	36.0	36.0	36.0	36.0
14	35.082062506906105	36.0	36.0	36.0	36.0	36.0
15	35.05523765064616	36.0	36.0	36.0	36.0	36.0
16	35.06566415915634	36.0	36.0	36.0	36.0	36.0
17	35.01751201506741	36.0	36.0	36.0	36.0	36.0
18	35.02015402627361	36.0	36.0	36.0	36.0	36.0
19	35.00398412310183	36.0	36.0	36.0	36.0	36.0
20	35.015638521218655	36.0	36.0	36.0	36.0	36.0
21	35.001719542067946	36.0	36.0	36.0	36.0	36.0
22	34.989112877694	36.0	36.0	36.0	36.0	36.0
23	34.9322835642817	36.0	36.0	36.0	32.0	36.0
24	34.897994529745596	36.0	36.0	36.0	32.0	36.0
25	34.864707423331396	36.0	36.0	36.0	32.0	36.0
26	34.81909672517248	36.0	36.0	36.0	32.0	36.0
27	34.817570864377174	36.0	36.0	36.0	32.0	36.0
28	34.78468726061429	36.0	36.0	36.0	32.0	36.0
29	34.75713609958197	36.0	36.0	36.0	32.0	36.0
30	34.72373477087878	36.0	36.0	36.0	32.0	36.0
31	34.72046081244328	36.0	36.0	36.0	32.0	36.0
32	34.66790862713499	36.0	36.0	36.0	32.0	36.0
33	34.632632563036424	36.0	36.0	36.0	32.0	36.0
34	34.615312987695035	36.0	36.0	36.0	32.0	36.0
35	34.557645630314596	36.0	36.0	36.0	32.0	36.0
36	34.540842538366896	36.0	36.0	36.0	32.0	36.0
37	34.52427782337013	36.0	36.0	36.0	32.0	36.0
38	34.46828034619286	36.0	36.0	36.0	32.0	36.0
39	34.47692151066426	36.0	36.0	36.0	32.0	36.0
40	34.43629189754261	36.0	36.0	36.0	32.0	36.0
41	34.41908778603682	36.0	36.0	36.0	32.0	36.0
42	34.320783366255014	36.0	36.0	36.0	32.0	36.0
43	34.32524548480533	36.0	36.0	36.0	32.0	36.0
44	34.23690695973789	36.0	36.0	36.0	32.0	36.0
45	34.19848109186576	36.0	36.0	36.0	32.0	36.0
46	34.16037057683498	36.0	36.0	36.0	32.0	36.0
47	34.10792019834949	36.0	36.0	36.0	32.0	36.0
48	34.05020317910427	36.0	36.0	36.0	32.0	36.0
49	34.020851775473744	36.0	36.0	36.0	32.0	36.0
50	33.420316917190085	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	2.0
20	9.0
21	32.0
22	108.0
23	276.0
24	733.0
25	1661.0
26	3425.0
27	6086.0
28	10054.0
29	14681.0
30	20870.0
31	29201.0
32	42019.0
33	66567.0
34	137597.0
35	472125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.806107473049785	18.23115720149893	12.096491816224512	25.86624350922677
2	16.142336930948385	20.904324463244357	36.496527989014716	26.45681061679254
3	17.843055702127394	25.07682088783219	29.064181911862384	28.015941498178027
4	12.017550503012613	16.031843187695777	36.08157954527114	35.869026764020475
5	14.239298178526955	37.10871106354608	32.95536515748398	15.696625600442985
6	33.77168369247272	36.09909565407403	16.71885320394714	13.410367449506111
7	29.7667379315311	30.865756363555096	21.114016333807605	18.253489371106195
8	28.55197176226369	31.823074640541215	19.699992674874945	19.924960922320153
9	27.983690841121035	13.406386843415438	18.74994568166101	39.85997663380252
10	16.39851062588228	26.221973358628027	30.781101982893798	26.59841403259589
11	37.464165860695985	20.773185572731663	22.674862529750563	19.08778603682179
12	25.056397255188735	23.83670185623635	28.41788472736257	22.689016161212344
13	29.6005313832726	19.087212658840766	25.52464786546567	25.78760809242096
14	24.050526043955774	19.68400116208908	25.168540162841456	31.096932631113695
15	25.32639020810751	26.986805330512375	22.46123359668041	25.225570864699698
16	25.96493841875248	25.222487087802943	23.782777115613825	25.029797377830754
17	24.008407753706948	25.449843378893956	25.142312281255002	25.39943658614409
18	25.06626771536232	24.221641452861462	26.556996442960102	24.15509438881612
19	25.89096489278624	24.10276529678551	25.63967585700859	24.366593953419656
20	26.153406833531985	23.38287147397696	25.089888756456098	25.37383293603496
21	27.8995390137402	23.489689576098737	24.383603142106182	24.22716826805488
22	26.33091024216427	23.504522344790768	25.173413454674126	24.991153958370838
23	24.560983119701625	23.181730143673736	25.856803023455623	26.400483713169017
24	25.09001246517449	24.37574803462438	25.53163224258918	25.002607257611952
25	25.11683171267096	23.87215804686181	25.60526524895956	25.40574499150766
26	24.783584721056474	24.93531132511212	26.088408683430263	24.192695270401142
27	25.497981750705552	24.084648516701616	25.061801512538473	25.35556822005436
28	24.43917459213826	24.24573514439671	26.11867100394825	25.196419259516773
29	24.652700610482114	24.064070203580947	25.976591356904212	25.306637829032724
30	24.454476489876697	24.69695897414886	25.98374262340471	24.86482191256973
31	25.21722179593195	24.378506030558807	24.76849954929116	25.635772624218088
32	24.952602310101945	24.538035482152768	24.57900794493301	25.930354262812276
33	24.613428621801955	23.956002751380034	25.46131554703544	25.969253079782572
34	25.57643193875562	24.133101738328268	25.46208293965079	24.828383383265315
35	26.235805648191146	23.973276253808542	25.549709098042527	24.241208999957784
36	24.772718807037585	24.875401979165375	24.86248898049392	25.489390233303123
37	25.81062893581517	24.644535052345102	24.839960070720515	24.704875941119212
38	24.851719826521197	24.435032983569673	25.287279255923444	25.425967933985678
39	25.679247789643327	24.12017188696979	24.67628409275738	25.5242962306295
40	26.047236938327277	24.657418593072762	25.048886990659636	24.24645747794033
41	24.523262931403746	25.079864866039337	25.70636795820333	24.69050424435359
42	26.299518395695237	25.47226150406055	24.776486684801842	23.451733415442376
43	25.293569894342156	24.058453248575297	25.23397439876836	25.414002458314194
44	24.922184906292873	24.94800946072619	24.441575049507406	25.688230583473526
45	25.022472412320347	25.310764906286316	24.502503004598776	25.164259676794565
46	24.673902517152243	25.01247774491765	24.624488164145045	25.689131573785062
47	25.04261714593625	24.808579796803436	25.131762406773056	25.017040650487253
48	26.185974038873404	25.316630143959472	23.95947531458156	24.537920502585557
49	25.05140022149274	25.567016453037084	24.072437065767453	25.309146259702725
50	25.308930906002814	25.704615743712434	23.890573960287494	25.095879389997254
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	160.0
1	98.0
2	36.0
3	107.5
4	179.0
5	212.5
6	246.0
7	227.5
8	209.0
9	311.5
10	414.0
11	592.5
12	771.0
13	1384.0
14	1997.0
15	2640.5
16	3284.0
17	3488.5
18	3693.0
19	3650.0
20	3607.0
21	3736.0
22	3865.0
23	4174.0
24	4483.0
25	4593.5
26	4704.0
27	5753.5
28	6803.0
29	7800.0
30	8797.0
31	9949.0
32	11101.0
33	12872.0
34	14643.0
35	17161.5
36	19680.0
37	23688.0
38	27696.0
39	30139.0
40	32582.0
41	35773.5
42	38965.0
43	39218.0
44	39471.0
45	44605.5
46	49740.0
47	54069.0
48	58398.0
49	60913.5
50	63429.0
51	60422.0
52	57415.0
53	57272.5
54	57130.0
55	58609.0
56	60088.0
57	59201.5
58	58315.0
59	53251.0
60	48187.0
61	42368.5
62	36550.0
63	31702.5
64	26855.0
65	22866.0
66	18877.0
67	16411.0
68	13945.0
69	12734.0
70	11523.0
71	8877.0
72	6231.0
73	5271.0
74	4311.0
75	3354.5
76	2398.0
77	2137.0
78	1876.0
79	1531.5
80	1187.0
81	939.0
82	691.0
83	587.5
84	484.0
85	337.5
86	191.0
87	138.0
88	85.0
89	75.0
90	65.0
91	48.5
92	32.0
93	25.0
94	18.0
95	17.5
96	17.0
97	15.5
98	14.0
99	11.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009063290322019945
2	7.449279716728723E-4
3	2.4830932389095745E-4
4	0.0
5	0.0
6	3.7246398583643615E-4
7	1.2415466194547873E-4
8	0.0
9	0.0012415466194547872
10	9.932372955638298E-4
11	0.0
12	0.0
13	2.4830932389095745E-4
14	1.2415466194547873E-4
15	0.005711114449492021
16	8.69082633618351E-4
17	0.0
18	2.4830932389095745E-4
19	0.0
20	8.69082633618351E-4
21	0.0
22	2.4830932389095745E-4
23	0.001365701281400266
24	3.7246398583643615E-4
25	0.001862319929182181
26	0.006331887759219415
27	0.005711114449492021
28	0.003352175872527925
29	0.002234783915018617
30	0.003476330534473404
31	0.006083578435328457
32	0.003476330534473404
33	0.004097103844200797
34	0.002234783915018617
35	0.002607247900855053
36	0.007076815730892287
37	0.0028555572247460103
38	0.005711114449492021
39	0.004221258506146276
40	0.002234783915018617
41	0.0029797118866914892
42	0.001738165267236702
43	0.002110629253073138
44	0.0014898559433457446
45	0.001862319929182181
46	0.0016140106052912235
47	0.0024830932389095744
48	0.0014898559433457446
49	3.7246398583643615E-4
50	9.932372955638298E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	805447.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.40752664743381
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.32614108240517	39.23738131589536
2	13.981037813517982	14.374611479144106
3	4.466268092510896	6.887993879410116
4	1.8709794928566035	3.847297125433122
5	0.9365410867934971	2.4072630437876663
6	0.5455167541376927	1.682620024497302
7	0.34886466324032456	1.2553988630312505
8	0.23119230497470072	0.9508019662934858
9	0.17007698744430189	0.7868913537742392
>10	0.9043623144212593	8.528815140315992
>50	0.09893579533870804	3.628832300760778
>100	0.10622781093345748	11.210483942465778
>500	0.012154211776688894	4.235890917267833
>1k	0.001701589648736445	0.9657186479229963
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1241	0.1540759354743391	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1217	0.15109622358764763	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1097	0.13619766415419016	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1066	0.13234886963388032	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1062	0.1318522509860984	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	1028	0.12763099247995213	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1017	0.12626529119855184	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	997	0.12378219795964229	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	975	0.12105079539684174	No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA	948	0.11769861952431383	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	939	0.11658122756680453	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	919	0.11409813432789494	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	908	0.11273243304649468	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	899	0.11161504108898536	No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA	885	0.10987687582174865	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	849	0.10540730799171144	No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC	841	0.1044140706961476	No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT	837	0.1039174520483657	No Hit
CTTTAAATCCTTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCA	817	0.10143435880945612	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.2415466194547873E-4	0.0
6	0.0	0.0	0.0	1.2415466194547873E-4	0.0
7	0.0	0.0	0.0	1.2415466194547873E-4	0.0
8	0.0	0.0	0.0	1.2415466194547873E-4	1.2415466194547873E-4
9	0.0	0.0	0.0	1.2415466194547873E-4	2.4830932389095745E-4
10	0.0	0.0	0.0	1.2415466194547873E-4	2.4830932389095745E-4
11	0.0	0.0	0.0	1.2415466194547873E-4	2.4830932389095745E-4
12	0.0	0.0	0.0	1.2415466194547873E-4	4.966186477819149E-4
13	0.0	0.0	0.0	1.2415466194547873E-4	4.966186477819149E-4
14	0.0	0.0	0.0	1.2415466194547873E-4	4.966186477819149E-4
15	0.0	0.0	0.0	1.2415466194547873E-4	4.966186477819149E-4
16	0.0	0.0	0.0	2.4830932389095745E-4	4.966186477819149E-4
17	0.0	0.0	0.0	2.4830932389095745E-4	4.966186477819149E-4
18	0.0	0.0	0.0	2.4830932389095745E-4	7.449279716728723E-4
19	0.0	0.0	0.0	2.4830932389095745E-4	8.69082633618351E-4
20	0.0	0.0	0.0	3.7246398583643615E-4	8.69082633618351E-4
21	0.0	0.0	0.0	6.207733097273936E-4	8.69082633618351E-4
22	0.0	0.0	0.0	0.0012415466194547872	8.69082633618351E-4
23	0.0	0.0	0.0	0.0023589385769640955	8.69082633618351E-4
24	0.0	0.0	0.0	0.003600485196418883	8.69082633618351E-4
25	0.0	0.0	0.0	0.004345413168091755	8.69082633618351E-4
26	0.0	0.0	0.0	0.004966186477819149	8.69082633618351E-4
27	0.0	0.0	0.0	0.0059594237733829785	9.932372955638298E-4
28	0.0	0.0	0.0	0.009187444983965426	9.932372955638298E-4
29	0.0	0.0	0.0	0.018002425982094415	0.0011173919575093085
30	0.0	0.0	0.0	0.03116282014831516	0.0011173919575093085
31	0.0	0.0	0.0	0.055621288551574465	0.0011173919575093085
32	0.0	0.0	0.0	0.08219038620790692	0.0011173919575093085
33	0.0	0.0	0.0	0.10640054528727526	0.0011173919575093085
34	0.0	0.0	0.0	0.13023824038080717	0.001365701281400266
35	0.0	0.0	0.0	0.15904212195215825	0.001365701281400266
36	0.0	0.0	0.0	0.20001316039416622	0.001365701281400266
37	0.0	0.0	0.0	0.2643252752819242	0.001365701281400266
38	0.0	0.0	0.0	0.35446155985434175	0.0016140106052912233
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCCG	50	0.0025801617	21.999317	32
TAGGACG	135	1.1277734E-10	21.184526	4
ACGTATG	65	4.9291656E-4	20.308323	22
AACGTAT	65	4.9312296E-4	20.307062	21
CAACGTA	65	4.9312296E-4	20.307062	20
TGCTACG	55	0.004479919	20.001863	10
GTTATAT	100	1.1221491E-6	19.800615	1
CTCGTTT	90	1.0010106E-5	19.556162	27
GTATATA	80	8.981682E-5	19.250599	1
CGTCGTA	275	0.0	19.201788	10
CCCTCGT	70	8.118416E-4	18.857727	25
ATACCGT	270	0.0	18.740158	6
GTATCAA	2110	0.0	18.664085	1
CCGTCGT	260	0.0	18.617119	9
GTCGTAG	260	0.0	18.617119	11
TAATACT	60	0.007411337	18.332764	4
CGTTTAG	60	0.007411337	18.332764	29
GGACGTG	160	1.1004886E-9	17.874445	6
GTATAAT	75	0.0012900946	17.600546	1
GTCGATA	75	0.0012906317	17.599453	36
>>END_MODULE