FastQCFastQC Report
Thu 2 Feb 2017
SRR4064145_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064145_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22.4390243902439024No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG22.4390243902439024No Hit
GTCTAAACACTTTACATCTACAAAA11.2195121951219512No Hit
CTTTTGTGCCCTCCAAAGGTGGATG11.2195121951219512No Hit
GGGGCATTCGTATTGCGCCGCTAGA11.2195121951219512No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11.2195121951219512No Hit
GGTCAGGCTGCCTCCAGCAAGGGGT11.2195121951219512No Hit
CTGTAGAACATATTAGATGAGTGAG11.2195121951219512No Hit
ACGCAGAGTACATGGGCTTCTCGCT11.2195121951219512No Hit
ACTCAAATTATTTAGCTCTGGTAAA11.2195121951219512No Hit
AATTCATGGCCCGGCGCAAGACGGA11.2195121951219512No Hit
TCTACAGTGTGGTTTTTATCATTTT11.2195121951219512No Hit
TTATTACATTGTAAAGACTCATCTT11.2195121951219512No Hit
CTCCTGAGCCCCAACCCAGAGGTGG11.2195121951219512No Hit
TGCTTCCACCTGCTGCATCCTGCCC11.2195121951219512No Hit
TCTCCAAACTTAGTAAAATAATCCT11.2195121951219512No Hit
CTCAGAGACTGTGGCAATTGCCTTA11.2195121951219512No Hit
CTCATGTACCTGGTCCCTGCCTGCA11.2195121951219512No Hit
GTGGTATCAACGCAGAGTACTTTTT11.2195121951219512No Hit
AGCAAACTCTGGATGGAAAAAGATC11.2195121951219512No Hit
CCATGCCAGTGTGTATGGGCAAGAC11.2195121951219512No Hit
CTCCGGCCCCTCCTGCTCCATCCAT11.2195121951219512No Hit
CCTCGGCATTGTACCCAGGGGCATA11.2195121951219512No Hit
GGTGTGTGCTGCTATGAACGCATCT11.2195121951219512No Hit
CCTCTAGCATTACAAGTATTAGAGG11.2195121951219512No Hit
TTCCATGAGAACTTAACAATTACCT11.2195121951219512No Hit
GTGCTGGACAGCGCTCCTACGGCGG11.2195121951219512No Hit
GAGTGATATTGAGTGTCAGGAGCTA11.2195121951219512No Hit
TAAGAGACAGTTGGACCCTCGTTTA11.2195121951219512No Hit
CGCCGCCCACAAAGATGAGTGGCAT11.2195121951219512No Hit
GGTGTACTGTTTGATCCACTTTGAC11.2195121951219512No Hit
CATCACCTGTTCTCATTTTTCTGCG11.2195121951219512No Hit
ATAATCGGCAGCCTGAGCCAGGAGA11.2195121951219512No Hit
ATACATGCCGACGGGCGCTGACCAC11.2195121951219512No Hit
GAACTACGACGGTATCTGATCGTCT11.2195121951219512No Hit
GAGTTTTTGTCCACGTCCATTGCTC11.2195121951219512No Hit
GTACTTTTTTTTTTTTTTTTTTTTT11.2195121951219512No Hit
CAGCTGGAGCTTCGAGGACCGTCAC11.2195121951219512No Hit
GGCCCGAGCCGCCTGGATACCGCAG11.2195121951219512No Hit
GTTGGCCTACTACGCGGCCAAGTCG11.2195121951219512No Hit
ACTGTAGCGTTCCTTCACTGCTTTC11.2195121951219512No Hit
GGGTAGGCACACGCTGAGCCAGTCA11.2195121951219512No Hit
GTTTTACTCCCCTCCTGAGTCTGAA11.2195121951219512No Hit
CAGGAATCGCTCATAGCCAACATCA11.2195121951219512No Hit
GTCCGATCTGGCCCTCAAGGGTCAG11.2195121951219512No Hit
AGATACAAGAGTCAAAGGATGGTTC11.2195121951219512No Hit
AGTTTCAGGAGACATGCCCTTCTTG11.2195121951219512No Hit
GGGCACCGGAGCCTGCCTCCCGGCT11.2195121951219512No Hit
GACTCTTCCTTCTTTGCCTCCACTT11.2195121951219512No Hit
CCGGTGTACTGTTTGATCCACTTTG11.2195121951219512No Hit
GGGAAAAGGGTGACCCGGAGAAGCC11.2195121951219512No Hit
ACTGTTCCACACTGTCAGACTCAGA11.2195121951219512No Hit
CATGATGCTCGTACTATTCGGTACC11.2195121951219512No Hit
CTTCATCATGGTGTACGGGATCTGT11.2195121951219512No Hit
AACTGACCATTGGCCATAAGGAACT11.2195121951219512No Hit
CAGCAGCTCCCTGAGCCCACAGAGT11.2195121951219512No Hit
CAAGAAGACCGACTCCCAGACAGAT11.2195121951219512No Hit
GTTCAGAAAAGAGGGTGCGGGCCGA11.2195121951219512No Hit
GGGTTCAACGGGTTACCCGCGCCTG11.2195121951219512No Hit
GTACAGAGCACATAAAAAAGTCAGC11.2195121951219512No Hit
GCTCAGGACTTGGGGACTGCATCTG11.2195121951219512No Hit
TCTTCATACGGAGATACAAAAGCAC11.2195121951219512No Hit
GTGCATTTCTCATTTTTCACGTATT11.2195121951219512No Hit
CTCTTTCGAGGCCCTGTAATTGGAA11.2195121951219512No Hit
CATCAATAGAAAACTCTTTCTTTGA11.2195121951219512No Hit
AAGCTATACAGGGTCAGAATCAAGG11.2195121951219512No Hit
GACCAACACAAGACAATTATAAAAT11.2195121951219512No Hit
TCCTACAATCGTGACCACGGAAGAC11.2195121951219512No Hit
GCTTAGGAGGTGGGATTGTATCACA11.2195121951219512No Hit
CTGCGGATGTATTTTTCACCCAAGA11.2195121951219512No Hit
CCCTGGAGACCTTGCTGAGCAATTA11.2195121951219512No Hit
TTCCTTCACTGCTTTCGCAGTTTCC11.2195121951219512No Hit
CCGGTGTTGGGTTAACAGAGAAGTT11.2195121951219512No Hit
GTCCTCGTCGTGAATGCCCCTACTT11.2195121951219512No Hit
CCATATTTCACGTCCTAAAGTGTAT11.2195121951219512No Hit
GCAAAGACTCCAGTGCGAGAGCAGA11.2195121951219512No Hit
AACGCAGAGTACTTTTTTTTTTTTT11.2195121951219512No Hit
AGTCTAATGTCTCTGCTTCATATCT11.2195121951219512No Hit
GTTAGCACCTCCAGTCACCATACAC11.2195121951219512No Hit
CTGCAGAGCCAAGCGGGAGAGCTGC11.2195121951219512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGCGC50.019.08
CCTACGG50.019.016
TCCTACG50.019.015
CTCCTAC50.019.014
GACAGCG50.019.07
TACGGCG50.019.018
CGCTCCT50.019.012
GCTGGAC50.019.03
TGCTGGA50.019.02
GGACAGC50.019.06
GCGCTCC50.019.011
CTGGACA50.019.04
TGGACAG50.019.05
CTACGGC50.019.017
AGCGCTC50.019.010
GCTCCTA50.019.013
CAGCGCT50.019.09
ACGGCGG50.019.019
GTGCTGG50.019.01
Produced by FastQC (version 0.11.5)