##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064145_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	82
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.402439024390244	NaN	NaN	NaN	NaN	NaN
2	28.475609756097562	NaN	NaN	NaN	NaN	NaN
3	29.353658536585368	NaN	NaN	NaN	NaN	NaN
4	29.28048780487805	NaN	NaN	NaN	NaN	NaN
5	28.390243902439025	NaN	NaN	NaN	NaN	NaN
6	32.146341463414636	NaN	NaN	NaN	NaN	NaN
7	31.426829268292682	NaN	NaN	NaN	NaN	NaN
8	31.158536585365855	NaN	NaN	NaN	NaN	NaN
9	30.426829268292682	NaN	NaN	NaN	NaN	NaN
10	31.109756097560975	NaN	NaN	NaN	NaN	NaN
11	33.34146341463415	NaN	NaN	NaN	NaN	NaN
12	31.365853658536587	NaN	NaN	NaN	NaN	NaN
13	29.841463414634145	NaN	NaN	NaN	NaN	NaN
14	32.02439024390244	NaN	NaN	NaN	NaN	NaN
15	31.0	NaN	NaN	NaN	NaN	NaN
16	32.109756097560975	NaN	NaN	NaN	NaN	NaN
17	29.79268292682927	NaN	NaN	NaN	NaN	NaN
18	30.414634146341463	NaN	NaN	NaN	NaN	NaN
19	31.71951219512195	NaN	NaN	NaN	NaN	NaN
20	29.98780487804878	NaN	NaN	NaN	NaN	NaN
21	30.4390243902439	NaN	NaN	NaN	NaN	NaN
22	31.963414634146343	NaN	NaN	NaN	NaN	NaN
23	31.79268292682927	NaN	NaN	NaN	NaN	NaN
24	31.353658536585368	NaN	NaN	NaN	NaN	NaN
25	30.402439024390244	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	1.0
19	0.0
20	2.0
21	3.0
22	2.0
23	4.0
24	3.0
25	2.0
26	1.0
27	1.0
28	1.0
29	4.0
30	5.0
31	5.0
32	9.0
33	6.0
34	18.0
35	14.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.46341463414634	17.073170731707318	10.975609756097562	30.48780487804878
2	20.73170731707317	21.951219512195124	35.36585365853659	21.951219512195124
3	28.04878048780488	17.073170731707318	30.48780487804878	24.390243902439025
4	19.51219512195122	9.75609756097561	30.48780487804878	40.243902439024396
5	17.073170731707318	37.80487804878049	32.926829268292686	12.195121951219512
6	32.926829268292686	35.36585365853659	14.634146341463413	17.073170731707318
7	23.170731707317074	28.04878048780488	24.390243902439025	24.390243902439025
8	20.73170731707317	32.926829268292686	21.951219512195124	24.390243902439025
9	29.268292682926827	15.853658536585366	15.853658536585366	39.02439024390244
10	18.29268292682927	19.51219512195122	31.70731707317073	30.48780487804878
11	36.58536585365854	14.634146341463413	29.268292682926827	19.51219512195122
12	23.170731707317074	20.73170731707317	26.82926829268293	29.268292682926827
13	29.268292682926827	18.29268292682927	31.70731707317073	20.73170731707317
14	21.951219512195124	20.73170731707317	36.58536585365854	20.73170731707317
15	26.82926829268293	23.170731707317074	30.48780487804878	19.51219512195122
16	23.170731707317074	23.170731707317074	19.51219512195122	34.146341463414636
17	25.609756097560975	23.170731707317074	23.170731707317074	28.04878048780488
18	15.853658536585366	30.48780487804878	24.390243902439025	29.268292682926827
19	25.609756097560975	29.268292682926827	24.390243902439025	20.73170731707317
20	25.609756097560975	23.170731707317074	23.170731707317074	28.04878048780488
21	26.82926829268293	23.170731707317074	29.268292682926827	20.73170731707317
22	28.04878048780488	21.951219512195124	31.70731707317073	18.29268292682927
23	20.73170731707317	23.170731707317074	31.70731707317073	24.390243902439025
24	23.170731707317074	23.170731707317074	31.70731707317073	21.951219512195124
25	19.51219512195122	25.609756097560975	34.146341463414636	20.73170731707317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.5
7	1.0
8	1.0
9	1.0
10	1.0
11	1.0
12	1.0
13	1.0
14	0.5
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	1.0
23	2.0
24	2.0
25	2.0
26	4.0
27	6.0
28	6.0
29	6.0
30	5.0
31	4.0
32	4.0
33	4.0
34	3.5
35	3.0
36	3.0
37	3.0
38	4.5
39	6.0
40	6.0
41	6.0
42	6.5
43	7.0
44	7.0
45	7.0
46	11.5
47	16.0
48	16.0
49	16.0
50	11.0
51	6.0
52	6.0
53	6.0
54	5.5
55	5.0
56	5.0
57	5.0
58	6.0
59	7.0
60	7.0
61	7.0
62	7.5
63	8.0
64	8.0
65	8.0
66	6.5
67	5.0
68	5.0
69	5.0
70	3.0
71	1.0
72	1.0
73	1.0
74	1.0
75	1.0
76	1.0
77	1.0
78	1.0
79	1.0
80	1.0
81	1.0
82	0.5
83	0.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	1.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	82.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	97.5609756097561
#Duplication Level	Percentage of deduplicated	Percentage of total
1	97.5	95.1219512195122
2	2.5	4.878048780487805
3	0.0	0.0
4	0.0	0.0
5	0.0	0.0
6	0.0	0.0
7	0.0	0.0
8	0.0	0.0
9	0.0	0.0
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2	2.4390243902439024	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	2	2.4390243902439024	No Hit
GTCTAAACACTTTACATCTACAAAA	1	1.2195121951219512	No Hit
CTTTTGTGCCCTCCAAAGGTGGATG	1	1.2195121951219512	No Hit
GGGGCATTCGTATTGCGCCGCTAGA	1	1.2195121951219512	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1	1.2195121951219512	No Hit
GGTCAGGCTGCCTCCAGCAAGGGGT	1	1.2195121951219512	No Hit
CTGTAGAACATATTAGATGAGTGAG	1	1.2195121951219512	No Hit
ACGCAGAGTACATGGGCTTCTCGCT	1	1.2195121951219512	No Hit
ACTCAAATTATTTAGCTCTGGTAAA	1	1.2195121951219512	No Hit
AATTCATGGCCCGGCGCAAGACGGA	1	1.2195121951219512	No Hit
TCTACAGTGTGGTTTTTATCATTTT	1	1.2195121951219512	No Hit
TTATTACATTGTAAAGACTCATCTT	1	1.2195121951219512	No Hit
CTCCTGAGCCCCAACCCAGAGGTGG	1	1.2195121951219512	No Hit
TGCTTCCACCTGCTGCATCCTGCCC	1	1.2195121951219512	No Hit
TCTCCAAACTTAGTAAAATAATCCT	1	1.2195121951219512	No Hit
CTCAGAGACTGTGGCAATTGCCTTA	1	1.2195121951219512	No Hit
CTCATGTACCTGGTCCCTGCCTGCA	1	1.2195121951219512	No Hit
GTGGTATCAACGCAGAGTACTTTTT	1	1.2195121951219512	No Hit
AGCAAACTCTGGATGGAAAAAGATC	1	1.2195121951219512	No Hit
CCATGCCAGTGTGTATGGGCAAGAC	1	1.2195121951219512	No Hit
CTCCGGCCCCTCCTGCTCCATCCAT	1	1.2195121951219512	No Hit
CCTCGGCATTGTACCCAGGGGCATA	1	1.2195121951219512	No Hit
GGTGTGTGCTGCTATGAACGCATCT	1	1.2195121951219512	No Hit
CCTCTAGCATTACAAGTATTAGAGG	1	1.2195121951219512	No Hit
TTCCATGAGAACTTAACAATTACCT	1	1.2195121951219512	No Hit
GTGCTGGACAGCGCTCCTACGGCGG	1	1.2195121951219512	No Hit
GAGTGATATTGAGTGTCAGGAGCTA	1	1.2195121951219512	No Hit
TAAGAGACAGTTGGACCCTCGTTTA	1	1.2195121951219512	No Hit
CGCCGCCCACAAAGATGAGTGGCAT	1	1.2195121951219512	No Hit
GGTGTACTGTTTGATCCACTTTGAC	1	1.2195121951219512	No Hit
CATCACCTGTTCTCATTTTTCTGCG	1	1.2195121951219512	No Hit
ATAATCGGCAGCCTGAGCCAGGAGA	1	1.2195121951219512	No Hit
ATACATGCCGACGGGCGCTGACCAC	1	1.2195121951219512	No Hit
GAACTACGACGGTATCTGATCGTCT	1	1.2195121951219512	No Hit
GAGTTTTTGTCCACGTCCATTGCTC	1	1.2195121951219512	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1	1.2195121951219512	No Hit
CAGCTGGAGCTTCGAGGACCGTCAC	1	1.2195121951219512	No Hit
GGCCCGAGCCGCCTGGATACCGCAG	1	1.2195121951219512	No Hit
GTTGGCCTACTACGCGGCCAAGTCG	1	1.2195121951219512	No Hit
ACTGTAGCGTTCCTTCACTGCTTTC	1	1.2195121951219512	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1	1.2195121951219512	No Hit
GTTTTACTCCCCTCCTGAGTCTGAA	1	1.2195121951219512	No Hit
CAGGAATCGCTCATAGCCAACATCA	1	1.2195121951219512	No Hit
GTCCGATCTGGCCCTCAAGGGTCAG	1	1.2195121951219512	No Hit
AGATACAAGAGTCAAAGGATGGTTC	1	1.2195121951219512	No Hit
AGTTTCAGGAGACATGCCCTTCTTG	1	1.2195121951219512	No Hit
GGGCACCGGAGCCTGCCTCCCGGCT	1	1.2195121951219512	No Hit
GACTCTTCCTTCTTTGCCTCCACTT	1	1.2195121951219512	No Hit
CCGGTGTACTGTTTGATCCACTTTG	1	1.2195121951219512	No Hit
GGGAAAAGGGTGACCCGGAGAAGCC	1	1.2195121951219512	No Hit
ACTGTTCCACACTGTCAGACTCAGA	1	1.2195121951219512	No Hit
CATGATGCTCGTACTATTCGGTACC	1	1.2195121951219512	No Hit
CTTCATCATGGTGTACGGGATCTGT	1	1.2195121951219512	No Hit
AACTGACCATTGGCCATAAGGAACT	1	1.2195121951219512	No Hit
CAGCAGCTCCCTGAGCCCACAGAGT	1	1.2195121951219512	No Hit
CAAGAAGACCGACTCCCAGACAGAT	1	1.2195121951219512	No Hit
GTTCAGAAAAGAGGGTGCGGGCCGA	1	1.2195121951219512	No Hit
GGGTTCAACGGGTTACCCGCGCCTG	1	1.2195121951219512	No Hit
GTACAGAGCACATAAAAAAGTCAGC	1	1.2195121951219512	No Hit
GCTCAGGACTTGGGGACTGCATCTG	1	1.2195121951219512	No Hit
TCTTCATACGGAGATACAAAAGCAC	1	1.2195121951219512	No Hit
GTGCATTTCTCATTTTTCACGTATT	1	1.2195121951219512	No Hit
CTCTTTCGAGGCCCTGTAATTGGAA	1	1.2195121951219512	No Hit
CATCAATAGAAAACTCTTTCTTTGA	1	1.2195121951219512	No Hit
AAGCTATACAGGGTCAGAATCAAGG	1	1.2195121951219512	No Hit
GACCAACACAAGACAATTATAAAAT	1	1.2195121951219512	No Hit
TCCTACAATCGTGACCACGGAAGAC	1	1.2195121951219512	No Hit
GCTTAGGAGGTGGGATTGTATCACA	1	1.2195121951219512	No Hit
CTGCGGATGTATTTTTCACCCAAGA	1	1.2195121951219512	No Hit
CCCTGGAGACCTTGCTGAGCAATTA	1	1.2195121951219512	No Hit
TTCCTTCACTGCTTTCGCAGTTTCC	1	1.2195121951219512	No Hit
CCGGTGTTGGGTTAACAGAGAAGTT	1	1.2195121951219512	No Hit
GTCCTCGTCGTGAATGCCCCTACTT	1	1.2195121951219512	No Hit
CCATATTTCACGTCCTAAAGTGTAT	1	1.2195121951219512	No Hit
GCAAAGACTCCAGTGCGAGAGCAGA	1	1.2195121951219512	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	1	1.2195121951219512	No Hit
AGTCTAATGTCTCTGCTTCATATCT	1	1.2195121951219512	No Hit
GTTAGCACCTCCAGTCACCATACAC	1	1.2195121951219512	No Hit
CTGCAGAGCCAAGCGGGAGAGCTGC	1	1.2195121951219512	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAGCGC	5	0.0	19.0	8
CCTACGG	5	0.0	19.0	16
TCCTACG	5	0.0	19.0	15
CTCCTAC	5	0.0	19.0	14
GACAGCG	5	0.0	19.0	7
TACGGCG	5	0.0	19.0	18
CGCTCCT	5	0.0	19.0	12
GCTGGAC	5	0.0	19.0	3
TGCTGGA	5	0.0	19.0	2
GGACAGC	5	0.0	19.0	6
GCGCTCC	5	0.0	19.0	11
CTGGACA	5	0.0	19.0	4
TGGACAG	5	0.0	19.0	5
CTACGGC	5	0.0	19.0	17
AGCGCTC	5	0.0	19.0	10
GCTCCTA	5	0.0	19.0	13
CAGCGCT	5	0.0	19.0	9
ACGGCGG	5	0.0	19.0	19
GTGCTGG	5	0.0	19.0	1
>>END_MODULE