FastQCFastQC Report
Thu 2 Feb 2017
SRR4064145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTACAATGCCGGTTTCCAACGTATGTGTTTTTCAGTGTAACTCACTC11.2195121951219512No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCGGTGGTCACCATGGTAGGC11.2195121951219512No Hit
ATCTGGGACAGACCCCGCCGAGCCCGACGAGGTGGACAAGTTCAAAGCCA11.2195121951219512No Hit
CAGTCCCCACAGTAGCTTAGCACAGAGAATGGCTTTGCTACTGTCTATTA11.2195121951219512No Hit
CTTTTTAACTGCAGCAACTTTAATATACGCTATTGGAGCTGGAATTACCG11.2195121951219512No Hit
CTCCCGCGCTGCGTGTTCCCTGGCAGAAAAAGTTGTATTTGCCTTCCCAC11.2195121951219512No Hit
GGTTCACGCCATTCTCCTGCCTCAGCCTCCCGAGTAGCTGTCTCTTATAC11.2195121951219512No Hit
TATCCCTACGGCACGGAACCCTTTGTGCGCTTCACGTTCAAGTATCGTAG11.2195121951219512No Hit
ATTTTATTCAGCAACTGCCGCGAGACCGAGAAGTAGGAATCCCTCCTGAG11.2195121951219512No Hit
CGCTGACATTGCCCTGGCTCCTATGGAAGCTGCTAAAGTTCGAATTCAAA11.2195121951219512No Hit
GCCATACTATGTTCCTGCTTTCTGCCGCCCTCAAGGTTGAATTTATCTTG11.2195121951219512No Hit
CCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGGCTTGCTTGGA11.2195121951219512No Hit
GAGCGGCATGAGCTCCAGTAAGCGCTTCCTGCGTTCTTGTTCGGCCTCCT11.2195121951219512No Hit
GTTTCTCTGTGTAGCCCTGACAGTCATGATCTCGCAGAGGCAGGCGGATT11.2195121951219512No Hit
GCTGCCACCAAGGTGCTCGGTCCTTCCGAGGGAGCTAAGGCCGCGTTGGG11.2195121951219512No Hit
CATCAGCATTGACAAGACCGGAGAGAACTTCCGTCTGATCTATGACACCA11.2195121951219512No Hit
TTCTGATAAAGCCTGAGAATGAATCTGGTTCCAGCCAGACCATGTCATGG11.2195121951219512No Hit
GTGTGAGGGCGCTGCCCCCCCCCGCACAGCCTGGACGGGATGTCTCCAAG11.2195121951219512No Hit
GTCCACCACGGGCTGTCTGAATGCACGTGGTGGACCTCTGACACTGGCAG11.2195121951219512No Hit
GCTTAACCACTGAGCCATCTCTCTAGCCTTATTAAATTCTTAAATGCTCA11.2195121951219512No Hit
AAAGTACTCTGCGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2195121951219512No Hit
GAGTTTATGTTGTATTGAATAAACTTTAAAGAAAAACAAAAAAAAAAAAA11.2195121951219512No Hit
AGGTGGGCCTGCTGACCCGTCCATGAGGCGGGCCCCCTGGCTGGGCCTGC11.2195121951219512No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA11.2195121951219512No Hit
GATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAACTA11.2195121951219512No Hit
GATATAACATATTTTATTCAGCAACTGCCGCGAGACCGAGAAGTAGGAAT11.2195121951219512No Hit
AGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACA11.2195121951219512No Hit
CGCCACCTGCCTGAGGATGCTGGTCCTTTCCTGCCGGTCAGTGTAGGGGA11.2195121951219512No Hit
CTCCTAGCCCGCCGACGAGCCCGCCCCGTGAGCCGCAGCAGCTTCGGTGC11.2195121951219512No Hit
GTACATGGGATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATAA11.2195121951219512No Hit
AGATATAACATATTTTATTCAGCAACTGCTGCGAGACCGAGAAGTAGGAA11.2195121951219512No Hit
CCTCCGAGCCCCCGCGTGCGCTCCTTTCCCCGCCATGAATGAGGAATACG11.2195121951219512No Hit
TCATTATCCAGGTTCTGCTCTGCTGCAATCTGGCTAGCTAAGGCAGCACA11.2195121951219512No Hit
GCCGGGCCCGGCTCCCCAGCCTGACGTTTCTCTTCAGCAACGGGTAGCAG11.2195121951219512No Hit
GTTCCTTATTGCGAAGCTCATAGATTCGACACTGCCGAGCCAACAACCGC11.2195121951219512No Hit
AACATATTTTATTCAGCAACTGCCGCGAGACCGAGAAGTAGGAATCCCTC11.2195121951219512No Hit
GTACATGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2195121951219512No Hit
CTTCTACACCAGCTTCGCCGCCTACATCTTCGGCCTGGGTCTCACCATCT11.2195121951219512No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT11.2195121951219512No Hit
ATGTCATTCCTGTGGCTGAAGGATATGTTATCGGCAGCTGTATTAAACAC11.2195121951219512No Hit
TTGATGAACTCTGCCCTTTTGTTTCTGACTCTGGGGGTCATAAAAAAGCT11.2195121951219512No Hit
TCTGAATTAGTCTGTTACAGTCAATACAAGCATGCTTGCTTTTAAACAAA11.2195121951219512No Hit
GTTAATGTTTGTCTTCTGCTTGATATGGGAAGTCCATGACAAATATTCTC11.2195121951219512No Hit
CCTTGAAGAAGGGTCCCTCACTCTGCTTCATTTCTGCAGTGCACTCTCAG11.2195121951219512No Hit
AGCTCACACAGCGCTGCCACGAGCCCAGCGTGTCCCTGTTCACATGGACG11.2195121951219512No Hit
GCGGGACCAGGCAGCAGAGGCCGGCTCCCCAGCCTGGGGCCACGGTCGCC11.2195121951219512No Hit
ATGTGCACTCCCTGCAAAAGCTCACATCTACCGTGAGGGCTGCGCGCAGA11.2195121951219512No Hit
GTGCCTTCTGCAAGACTCCTTCTGTACAGCTATGTTTTATCCAAGCTGAA11.2195121951219512No Hit
ATTCCAGAGAATGTCGAAATCACTCTGAAGGGGCGCACAGTCATTGTGAA11.2195121951219512No Hit
CGGGGTACCCCGCTGCTCGGAGGAAGCGGCCCCGGAATTTCTGGGGCTCC11.2195121951219512No Hit
ACTCACATCTGTGCAGCACTCACACTGAGACGCAGTGCATGAGAAGGGTG11.2195121951219512No Hit
CCTACAGATCCTGGCAGAGTTCAAGGAGAAGGCAAGGGCTCTGTACCAGG11.2195121951219512No Hit
GTGGCCTGCCTCCTCACCCGAGTACAGGGTCCATCCACTAAAGCCCTGTT11.2195121951219512No Hit
ATAACTTTATAGATGCAACACTGTTAGCATGAGTAACAAGAATTCCAATT11.2195121951219512No Hit
TTGTTACTCTTGCCAATAACAAAAATGTTGGACAGCCGAGTGGCAAAGCT11.2195121951219512No Hit
CCCTGAAGCAGCCGACAACATTGATGTGAGCATCCAGGATCTCTTCAGCT11.2195121951219512No Hit
TCTGCAGCCTTGGCTGCCACCTTGGGGAGGCTCTTCTCTTCAAGGAGGCG11.2195121951219512No Hit
GTACATGGGCTATTTTGTTGGTTTTCGGAACTGAGGCCATGATTAAGAGG11.2195121951219512No Hit
GGTAATATCTCAGGGGTGGCCACTCGCCATAAGATGTACAAACCCACTCA11.2195121951219512No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA11.2195121951219512No Hit
GCCTCACCATATGGCTCGGACACCACTCCCTGCTGCTTTGCCTACCTCTC11.2195121951219512No Hit
GTTCAGGATAAGACAGAAGTGCTGAATTACACGACCATTCCGGTCTACCT11.2195121951219512No Hit
CCGCCGAGCCCTCCTGCCGTCAGGGTGTTCAGCATAGGCATCCAGGTACC11.2195121951219512No Hit
GCTCCCCTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTT11.2195121951219512No Hit
ACATCTAGGTGGTCCATAATTTAACCCCTGCAACGCCCAGGTCTATAGAA11.2195121951219512No Hit
GTTTCAGGCTCTTTGATCTCTTTATAAGTTAACTAATACGTCTATTTTCT11.2195121951219512No Hit
GTATTAAACACATTCCAATCGCAGGAAGAGATATAACATATTTTATTCAG11.2195121951219512No Hit
GTCGCTGGCAGGGCATGGTACTGGTGTCGCGAGATCCACTCTGGAGGGCT11.2195121951219512No Hit
ATAGATAAGTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTAT11.2195121951219512No Hit
GGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTT11.2195121951219512No Hit
GTCAGTGGCTGCGGACGAGCCCTCGGGGGTGCGAGCGTGACCGCGGCCAA11.2195121951219512No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC11.2195121951219512No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT11.2195121951219512No Hit
GCGTGTTGCATTGGCAAACACCGGAACTCCGTTTAGTGCGTCTGATGAAG11.2195121951219512No Hit
CAGCACAAGACCGAGTTATTCATTATCAGGTCAGACCGAAACTCACAGAC11.2195121951219512No Hit
ATTAAACACATTCCAATCGCAGGAAGAGATATAACATATTTTATTCAGCA11.2195121951219512No Hit
GAGTACATGGGAGTCGACCGGAATCCCCGAGAGGAGCAATGGCTCTAACA11.2195121951219512No Hit
ATGGAGGCTTGCTCTACAACGCAGGCAACATCTGCCACCATTTCTTCACC11.2195121951219512No Hit
CCGTTGCCTCCTCGCCCTGGCTTATACTCTTGCAGTCCCCCGCTCCCCAG11.2195121951219512No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG11.2195121951219512No Hit
GGTGCGGACTGTGCGGCGCTCCCCCCCGTGGCTCCGCTCCCTCCTAGCCC11.2195121951219512No Hit
GTGTAGTGTGGTGACCCTTGCCGCCTCCGAGCCCGAAACTCCTCCAGTTC11.2195121951219512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGAG50.044.026
AGCTTCG50.044.040
CAGCAGC50.044.036
TCCTAGC50.044.02
CGCCCCG50.044.022
CTCCTAG50.044.01
TCGGTGC50.044.044
CCGCCGA50.044.09
CCGCCCC50.044.021
TAGCCCG50.044.05
GAGCCGC50.044.030
GAGCCCG50.044.017
CCGCAGC50.044.033
GTGAGCC50.044.028
AGCAGCT50.044.037
CCCGTGA50.044.025
GCAGCTT50.044.038
CCCGCCG50.044.08
CCCGCCC50.044.020
CCCCGTG50.044.024