FastQCFastQC Report
Thu 2 Feb 2017
SRR4064144_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064144_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28646
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGG4251.4836277316204705No Hit
TATCAACGCAGAGTACTTTTTTTTT1070.37352509949033025No Hit
GTATCAACGCAGAGTACTTTTTTTT1030.3595615443691964No Hit
GTATCAACGCAGAGTACATGGGGGG890.310689101445228No Hit
GGTATCAACGCAGAGTACATGGGGG590.20596243803672415No Hit
GTATCAACGCAGAGTACATGGGGAC580.2024715492564407No Hit
GGTATCAACGCAGAGTACATGGGGA500.174544439014173No Hit
GGTATCAACGCAGAGTACATGGGCC440.15359910633247223No Hit
GTATCAACGCAGAGTACATGGGCCC440.15359910633247223No Hit
GGTATCAACGCAGAGTACTTTTTTT410.14312643999162186No Hit
GTATCAACGCAGAGTACATGGGAGA390.13614466243105494No Hit
GTATCAACGCAGAGTACATGGGCCT370.12916288487048802No Hit
TTCTTAGACAGGCCACCCCCACCTG360.12567199609020457No Hit
GTATCAACGCAGAGTACATGGGAAG350.12218110730992111No Hit
GTATCAACGCAGAGTACATGGGCCA320.11170844096907073No Hit
GTATAGCGCTGGCTGTCCTGGAGCT310.10821755218878726No Hit
GTTTTATAGTTGTCCATGTAGTCTT310.10821755218878726No Hit
GTGTGGGGCGGAGGCGCCTGCGGGG310.10821755218878726No Hit
GTCTAAGAAACCTGCCACACTGCTA300.1047266634085038No Hit
GTATCAACGCAGAGTACATGGGGAG300.1047266634085038No Hit
TATCAACGCAGAGTACATGGGGGGG290.10123577462822035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA451.5548994E-619.01
GTATCAA1400.013.5714281
TATCAAC1802.5465852E-1110.5555562
ATCAACG2001.9645086E-109.53
TCAACGC2053.1468517E-109.2682924
CAACGCA2053.1468517E-109.2682925
AACGCAG2104.965841E-109.0476196
GCAGAGT2501.5133992E-97.989
ACGCAGA2406.146365E-97.9166667
CAGAGTA2406.146365E-97.91666610
CGCAGAG2406.146365E-97.9166668
AGAGTAC2459.027644E-97.755101711
AGTACAT2302.1350024E-77.434782513
GAGTACA2302.1350024E-77.434782512
GTACATG2703.0545198E-66.333333514
ACATGGG2857.3838146E-66.016
TACATGG2909.79172E-65.896551615
Produced by FastQC (version 0.11.5)