##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064144_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28646
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.345632898135865	32.0	32.0	32.0	32.0	32.0
2	31.042030300914615	32.0	32.0	32.0	32.0	32.0
3	31.047231725197236	32.0	32.0	32.0	32.0	32.0
4	31.02970746352021	32.0	32.0	32.0	32.0	32.0
5	31.044264469733996	32.0	32.0	32.0	32.0	32.0
6	34.6798156810724	36.0	36.0	36.0	32.0	36.0
7	34.620610207358794	36.0	36.0	36.0	32.0	36.0
8	34.57107449556657	36.0	36.0	36.0	32.0	36.0
9	34.70470571807582	36.0	36.0	36.0	32.0	36.0
10	34.57735809537108	36.0	36.0	36.0	32.0	36.0
11	34.75699923200447	36.0	36.0	36.0	32.0	36.0
12	34.58856384835579	36.0	36.0	36.0	32.0	36.0
13	34.585072959575506	36.0	36.0	36.0	32.0	36.0
14	34.516651539481956	36.0	36.0	36.0	32.0	36.0
15	34.49636947566851	36.0	36.0	36.0	32.0	36.0
16	34.481323745025485	36.0	36.0	36.0	32.0	36.0
17	34.438141450813376	36.0	36.0	36.0	32.0	36.0
18	34.4888989736787	36.0	36.0	36.0	32.0	36.0
19	34.42128045800461	36.0	36.0	36.0	32.0	36.0
20	34.427529148921316	36.0	36.0	36.0	32.0	36.0
21	34.32891154087831	36.0	36.0	36.0	32.0	36.0
22	34.33840675836068	36.0	36.0	36.0	32.0	36.0
23	34.310130559240385	36.0	36.0	36.0	32.0	36.0
24	34.3410598338337	36.0	36.0	36.0	32.0	36.0
25	34.017419535013616	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	2.0
9	4.0
10	2.0
11	3.0
12	2.0
13	1.0
14	2.0
15	3.0
16	10.0
17	13.0
18	17.0
19	17.0
20	18.0
21	45.0
22	50.0
23	75.0
24	95.0
25	161.0
26	271.0
27	342.0
28	490.0
29	628.0
30	877.0
31	1164.0
32	1727.0
33	2341.0
34	5471.0
35	14813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.624388881128645	17.547841877357172	12.302695907249616	24.52507333426456
2	16.536917437598184	20.729621225344737	39.071391167743066	23.662070169314017
3	18.91363541157579	26.572645395517696	29.951825734832088	24.561893458074426
4	12.993088040215039	16.0196886127208	35.7955735530266	35.19164979403756
5	13.97800663292023	39.08535521033339	32.3302496072613	14.606388549485075
6	32.38600656378745	36.41156343830738	17.802527756441588	13.399902241463584
7	29.629888268156424	33.187849162011176	19.085195530726256	18.097067039106147
8	30.20185793113082	32.68492002514493	17.985611510791365	19.127610532932877
9	30.75337920435891	13.202472844120008	18.050364988997938	37.993782962523134
10	17.82233555734097	28.78401509064869	30.49219268522723	22.90145666678311
11	35.65964581368542	23.256138880156485	21.50267211568689	19.5815431904712
12	26.427947598253276	26.791266375545852	26.319650655021835	20.46113537117904
13	30.3381777529346	22.163219675796533	24.832308552263836	22.666294019005033
14	25.817724350013975	20.181017612524464	27.32736930388594	26.673888733575623
15	26.402571089219588	27.813875497799202	23.146789631803255	22.636763781177947
16	24.325740637227504	28.511039686975963	24.060229178311907	23.10299049748463
17	23.49262907846014	26.96499685600503	24.568574023614893	24.973800041919933
18	23.82083711830061	27.768849137027463	26.43421144574104	21.97610229893089
19	26.182656697645168	25.082104674725734	27.087555027601145	21.64768360002795
20	28.500558971492453	25.052403577417554	24.51439351593069	21.93264393515931
21	30.22955172775235	23.514202858041298	25.016596205583312	21.23964920862304
22	28.547970092935504	25.525819299839288	25.026203619593318	20.90000698763189
23	27.31211348310681	24.92924775514482	24.78949023444324	22.969148527305126
24	25.751222921034238	25.545073375262056	26.39412997903564	22.309573724668063
25	25.794052901918306	25.133652468639717	25.661273978825257	23.411020650616724
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	5.0
3	8.0
4	8.0
5	8.0
6	26.0
7	44.0
8	44.0
9	44.0
10	47.5
11	51.0
12	51.0
13	51.0
14	101.0
15	151.0
16	151.0
17	151.0
18	215.0
19	279.0
20	279.0
21	279.0
22	428.0
23	577.0
24	577.0
25	577.0
26	841.5
27	1106.0
28	1106.0
29	1106.0
30	1399.5
31	1693.0
32	1693.0
33	1693.0
34	1933.5
35	2174.0
36	2174.0
37	2174.0
38	2362.0
39	2550.0
40	2550.0
41	2550.0
42	2692.5
43	2835.0
44	2835.0
45	2835.0
46	3074.5
47	3314.0
48	3314.0
49	3314.0
50	3528.0
51	3742.0
52	3742.0
53	3742.0
54	3553.5
55	3365.0
56	3365.0
57	3365.0
58	3080.0
59	2795.0
60	2795.0
61	2795.0
62	2202.0
63	1609.0
64	1609.0
65	1609.0
66	1329.5
67	1050.0
68	1050.0
69	1050.0
70	759.0
71	468.0
72	468.0
73	468.0
74	323.5
75	179.0
76	179.0
77	179.0
78	147.0
79	115.0
80	115.0
81	115.0
82	88.5
83	62.0
84	62.0
85	62.0
86	41.0
87	20.0
88	20.0
89	20.0
90	12.5
91	5.0
92	5.0
93	5.0
94	8.0
95	11.0
96	11.0
97	11.0
98	226.0
99	441.0
100	441.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0349088878028346
2	0.0034908887802834604
3	0.0
4	0.0
5	0.0034908887802834604
6	0.013963555121133842
7	0.020945332681700762
8	0.041890665363401525
9	0.0523633317042519
10	0.06632688682538575
11	0.05934510926481882
12	0.07330866438595267
13	0.07679955316623611
14	0.1047266634085038
15	0.0698177756056692
16	0.07679955316623611
17	0.0698177756056692
18	0.08378133072680305
19	0.08378133072680305
20	0.07679955316623611
21	0.0872722195070865
22	0.08378133072680305
23	0.0872722195070865
24	0.09076310828736997
25	0.09425399706765342
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	28646.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.63987991342596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.4255774820254	29.403756196327585
2	15.335780939268778	13.998464008936676
3	6.853296619244302	9.383509041401942
4	3.877925653969711	7.079522446414857
5	2.715312834633624	6.196327585003142
6	1.6215389322319105	4.4404105285205615
7	1.1932078935291417	3.8120505480695384
8	0.9255009943399112	3.3791803393143893
9	0.6654428636989446	2.7333659149619494
>10	2.340523175768701	16.637575926830973
>50	0.02294630564479119	0.7191230887383928
>100	0.02294630564479119	2.2167143754799974
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	425	1.4836277316204705	No Hit
TATCAACGCAGAGTACTTTTTTTTT	107	0.37352509949033025	No Hit
GTATCAACGCAGAGTACTTTTTTTT	103	0.3595615443691964	No Hit
GTATCAACGCAGAGTACATGGGGGG	89	0.310689101445228	No Hit
GGTATCAACGCAGAGTACATGGGGG	59	0.20596243803672415	No Hit
GTATCAACGCAGAGTACATGGGGAC	58	0.2024715492564407	No Hit
GGTATCAACGCAGAGTACATGGGGA	50	0.174544439014173	No Hit
GGTATCAACGCAGAGTACATGGGCC	44	0.15359910633247223	No Hit
GTATCAACGCAGAGTACATGGGCCC	44	0.15359910633247223	No Hit
GGTATCAACGCAGAGTACTTTTTTT	41	0.14312643999162186	No Hit
GTATCAACGCAGAGTACATGGGAGA	39	0.13614466243105494	No Hit
GTATCAACGCAGAGTACATGGGCCT	37	0.12916288487048802	No Hit
TTCTTAGACAGGCCACCCCCACCTG	36	0.12567199609020457	No Hit
GTATCAACGCAGAGTACATGGGAAG	35	0.12218110730992111	No Hit
GTATCAACGCAGAGTACATGGGCCA	32	0.11170844096907073	No Hit
GTATAGCGCTGGCTGTCCTGGAGCT	31	0.10821755218878726	No Hit
GTTTTATAGTTGTCCATGTAGTCTT	31	0.10821755218878726	No Hit
GTGTGGGGCGGAGGCGCCTGCGGGG	31	0.10821755218878726	No Hit
GTCTAAGAAACCTGCCACACTGCTA	30	0.1047266634085038	No Hit
GTATCAACGCAGAGTACATGGGGAG	30	0.1047266634085038	No Hit
TATCAACGCAGAGTACATGGGGGGG	29	0.10123577462822035	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	45	1.5548994E-6	19.0	1
GTATCAA	140	0.0	13.571428	1
TATCAAC	180	2.5465852E-11	10.555556	2
ATCAACG	200	1.9645086E-10	9.5	3
TCAACGC	205	3.1468517E-10	9.268292	4
CAACGCA	205	3.1468517E-10	9.268292	5
AACGCAG	210	4.965841E-10	9.047619	6
GCAGAGT	250	1.5133992E-9	7.98	9
ACGCAGA	240	6.146365E-9	7.916666	7
CAGAGTA	240	6.146365E-9	7.916666	10
CGCAGAG	240	6.146365E-9	7.916666	8
AGAGTAC	245	9.027644E-9	7.7551017	11
AGTACAT	230	2.1350024E-7	7.4347825	13
GAGTACA	230	2.1350024E-7	7.4347825	12
GTACATG	270	3.0545198E-6	6.3333335	14
ACATGGG	285	7.3838146E-6	6.0	16
TACATGG	290	9.79172E-6	5.8965516	15
>>END_MODULE