FastQCFastQC Report
Thu 2 Feb 2017
SRR4064144_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064144_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28646
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTTGACTCTGGGCATGGGCCCCTGACCACCAGTCCCAGAAGCAGGTG450.1570899951127557No Hit
TTCTTAGACAGGCCACCCCCACCTGCATCCTCCACGAACCAGCACTCGAT320.11170844096907073No Hit
CTTTTATAGTGAGTCAGTCCTACGTGGCTCTGGCCAGGTAACTGTGGGGC320.11170844096907073No Hit
CCCTTACACATACTTCTTGCCACAGTGCTCAGGTGCCAGCAAGAAGGGAT310.10821755218878726No Hit
GTACATGGGTGAGGACGGTCAGCACCACTGTTGCCATGGTGATGACGACA290.10123577462822035No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA353.140576E-431.4285721
GTATCAA1200.029.3333361
TGGGAGG500.002519916522.019
ATGGGGG755.237251E-520.53333312
TATCAAC1850.019.0270272
TCAACGC1901.8189894E-1218.5263164
ATCAACG1901.8189894E-1218.5263163
AACGCAG1951.8189894E-1218.0512836
CAACGCA1951.8189894E-1218.0512835
ACGCAGA2652.8376235E-1013.283026
CATGGGC1353.0399315E-413.03703716
CGCAGAG2755.12955E-1012.87
CAGAGTA2806.82121E-1012.5714299
AGAGTAC2859.0221874E-1012.35087810
GCAGAGT2901.1878001E-912.1379318
AGTACAT2604.343019E-811.84615412
GAGTACA2604.343019E-811.84615411
CATGGGG1600.001268967511.00000111
GTACATG3301.0570511E-710.01
TACATGG3301.0570511E-710.02
Produced by FastQC (version 0.11.5)