FastQCFastQC Report
Thu 2 Feb 2017
SRR4064143_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064143_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126355
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT24270.2154738071034443No Hit
TATCAACGCAGAGTACTTTTTTTTT15780.14009792649741865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAA953.0377123E-1015.00203513
CGATAAC953.0377123E-1015.00070210
CCGATAA1006.5847416E-1014.2506669
CGTCGTA1301.8189894E-1213.88526410
CGAACGA1051.364242E-913.57447616
GCACCGA500.00150007713.2988496
CTAACAC655.4725242E-513.1480353
TAAGACT802.0049392E-613.0584694
GTATCAA16450.013.0476451
GATAACG1102.739398E-912.95572811
AACGAAC1207.366907E-1012.66894714
TAGGCAG751.4803914E-512.6644465
CGCATCG1600.012.47044213
AATTCCG1301.9826984E-1012.4208985
ACGAACG1155.298716E-912.39408715
ATAACGA1155.305992E-912.39298412
CGACGGG853.932655E-612.29633214
TACCGTC1551.8189894E-1212.2586387
GCATCGC1800.012.14107514
GTCCGGC550.003065446612.09201211
Produced by FastQC (version 0.11.5)