##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064143_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1126355
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.215447172516658	32.0	32.0	32.0	32.0	32.0
2	30.753054765149532	32.0	32.0	32.0	32.0	32.0
3	30.768783376466565	32.0	32.0	32.0	32.0	32.0
4	30.806971159181607	32.0	32.0	32.0	32.0	32.0
5	30.695868531679622	32.0	32.0	32.0	32.0	32.0
6	34.35848023047796	36.0	36.0	36.0	32.0	36.0
7	34.30045411970471	36.0	36.0	36.0	32.0	36.0
8	34.24742554523219	36.0	36.0	36.0	32.0	36.0
9	34.406672851809596	36.0	36.0	36.0	32.0	36.0
10	34.06623222696219	36.0	36.0	36.0	32.0	36.0
11	34.38554896102916	36.0	36.0	36.0	32.0	36.0
12	34.17960056998016	36.0	36.0	36.0	32.0	36.0
13	34.26515263837778	36.0	36.0	36.0	32.0	36.0
14	34.14740823275078	36.0	36.0	36.0	32.0	36.0
15	34.08424253454728	36.0	36.0	36.0	32.0	36.0
16	34.08326593303177	36.0	36.0	36.0	32.0	36.0
17	33.998180857722474	36.0	36.0	36.0	32.0	36.0
18	34.003355957935106	36.0	36.0	36.0	32.0	36.0
19	34.02821401778303	36.0	36.0	36.0	32.0	36.0
20	33.990559814623275	36.0	36.0	36.0	32.0	36.0
21	33.990300571311884	36.0	36.0	36.0	32.0	36.0
22	33.96714890065743	36.0	36.0	36.0	32.0	36.0
23	33.904177634937476	36.0	36.0	36.0	32.0	36.0
24	33.89243622126239	36.0	36.0	36.0	32.0	36.0
25	33.49252500321835	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	9.0
5	19.0
6	36.0
7	27.0
8	51.0
9	78.0
10	81.0
11	32.0
12	76.0
13	57.0
14	113.0
15	200.0
16	349.0
17	452.0
18	578.0
19	799.0
20	1309.0
21	1932.0
22	2970.0
23	4658.0
24	7084.0
25	9897.0
26	14016.0
27	18326.0
28	24400.0
29	32481.0
30	42470.0
31	57043.0
32	78997.0
33	110755.0
34	232710.0
35	484346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.05984460652482	17.39126187033491	11.675560166122517	25.873333357017753
2	16.67696596791235	19.809196565715755	37.59555709453161	25.91828037184029
3	18.82319098952886	23.94902400176147	28.72451688397935	28.50326812473032
4	12.746167812734448	15.554618379405404	36.12515148959217	35.57406231826798
5	14.751337164288303	36.49464068815127	33.58360029264897	15.170421854911458
6	33.87476436517781	35.47739193207679	17.03265723269044	13.615186470054955
7	30.215219144167342	30.446990874765117	20.800935611859607	18.536854369207926
8	28.116723831989017	32.830953467842065	19.363058116794882	19.68926458337404
9	27.575842092827845	14.284711847123802	18.506056886232862	39.633389173815495
10	16.0863331592244	26.36444553410581	31.61347461029801	25.93574669637178
11	36.898703536737564	21.065595977810446	22.87069566646384	19.165004818988145
12	25.115754724356286	23.29983582906323	28.747947863290385	22.8364615832901
13	29.647558620297538	19.559821398429687	25.11340275791243	25.679217223360347
14	23.745562382507522	19.64927274601014	25.071517158787636	31.533647712694695
15	25.148599465033044	27.270433703451346	22.13743373987387	25.44353309164174
16	25.683876670768164	25.637590451357273	23.79733565504773	24.881197222826835
17	24.06709705388783	25.817547681212595	25.161839394555873	24.953515870343704
18	24.813767685850255	24.926342276512337	26.123702326837712	24.136187710799696
19	25.817855822813602	24.692404469464346	25.33798191743432	24.15175779028773
20	25.983983309802028	24.287774412213416	24.80756603352789	24.920676244456665
21	26.804586263421392	24.076283722851798	24.39364391156206	24.725486102164748
22	26.123122797602438	24.25369487453331	24.67351649699061	24.949665830873638
23	24.625939316823334	24.322496776756033	25.31688197906735	25.734681927353282
24	25.07585483849472	24.722768890409196	25.218368643408954	24.983007627687126
25	24.94457974341865	24.4823814672885	25.21743843353701	25.355600355755843
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	71.0
2	213.5
3	356.0
4	356.0
5	356.0
6	796.0
7	1236.0
8	1236.0
9	1236.0
10	1466.5
11	1697.0
12	1697.0
13	1697.0
14	2214.5
15	2732.0
16	2732.0
17	2732.0
18	4779.0
19	6826.0
20	6826.0
21	6826.0
22	11199.0
23	15572.0
24	15572.0
25	15572.0
26	23405.5
27	31239.0
28	31239.0
29	31239.0
30	39733.5
31	48228.0
32	48228.0
33	48228.0
34	59871.5
35	71515.0
36	71515.0
37	71515.0
38	82293.0
39	93071.0
40	93071.0
41	93071.0
42	106046.0
43	119021.0
44	119021.0
45	119021.0
46	132814.5
47	146608.0
48	146608.0
49	146608.0
50	149942.5
51	153277.0
52	153277.0
53	153277.0
54	143255.0
55	133233.0
56	133233.0
57	133233.0
58	122162.0
59	111091.0
60	111091.0
61	111091.0
62	96609.5
63	82128.0
64	82128.0
65	82128.0
66	67387.0
67	52646.0
68	52646.0
69	52646.0
70	39834.0
71	27022.0
72	27022.0
73	27022.0
74	20553.0
75	14084.0
76	14084.0
77	14084.0
78	11135.0
79	8186.0
80	8186.0
81	8186.0
82	5671.0
83	3156.0
84	3156.0
85	3156.0
86	2401.5
87	1647.0
88	1647.0
89	1647.0
90	1204.0
91	761.0
92	761.0
93	761.0
94	533.5
95	306.0
96	306.0
97	306.0
98	476.0
99	646.0
100	646.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03897527866436425
2	0.005770827137092658
3	0.0018644210750607046
4	0.0035512782382108657
5	0.00807915799192972
6	0.012607037745648574
7	0.021929143120952097
8	0.033825925218958504
9	0.0469656547003387
10	0.05531115856013424
11	0.053269173573162985
12	0.062058587212734884
13	0.06401179024375085
14	0.06667524892240902
15	0.060194166137674185
16	0.06658646696645375
17	0.062324933080600706
18	0.07803933928468379
19	0.07555344451793618
20	0.08025888818356558
21	0.07280120388332276
22	0.07786177537277324
23	0.08309991077413427
24	0.0754646625619809
25	0.07670760994535471
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1126355.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.703474562367376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.46900663251941	47.825137584734414
2	11.150260063401308	13.09118015991319
3	3.0898978871678797	5.441632260590169
4	1.3105102282136154	3.0772601538264093
5	0.7413750787654713	2.1760646538740973
6	0.474823187127478	1.6724262532296066
7	0.31097031845182066	1.2778526725221413
8	0.23333809861447122	1.0958205709156625
9	0.1813594523533748	0.9581786998084094
>10	0.8917405077594383	9.304233214530523
>50	0.06934856800632859	2.848906699484717
>100	0.0703778010564989	8.170486978017067
>500	0.006688168886097015	2.7034478026318896
>1k	3.0400767664077343E-4	0.3573722959217321
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2427	0.2154738071034443	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1578	0.14009792649741865	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.6634586786581494E-4
13	0.0	0.0	0.0	8.878195595527165E-5	3.551278238210866E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGAA	95	3.0377123E-10	15.002035	13
CGATAAC	95	3.0377123E-10	15.000702	10
CCGATAA	100	6.5847416E-10	14.250666	9
CGTCGTA	130	1.8189894E-12	13.885264	10
CGAACGA	105	1.364242E-9	13.574476	16
GCACCGA	50	0.001500077	13.298849	6
CTAACAC	65	5.4725242E-5	13.148035	3
TAAGACT	80	2.0049392E-6	13.058469	4
GTATCAA	1645	0.0	13.047645	1
GATAACG	110	2.739398E-9	12.955728	11
AACGAAC	120	7.366907E-10	12.668947	14
TAGGCAG	75	1.4803914E-5	12.664446	5
CGCATCG	160	0.0	12.470442	13
AATTCCG	130	1.9826984E-10	12.420898	5
ACGAACG	115	5.298716E-9	12.394087	15
ATAACGA	115	5.305992E-9	12.392984	12
CGACGGG	85	3.932655E-6	12.296332	14
TACCGTC	155	1.8189894E-12	12.258638	7
GCATCGC	180	0.0	12.141075	14
GTCCGGC	55	0.0030654466	12.092012	11
>>END_MODULE