FastQCFastQC Report
Thu 2 Feb 2017
SRR4064143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126355
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG250.002354181435.195562
GTATCAA17100.026.1148261
GGTATCA6600.021.9982031
AACGAAT1101.2623968E-720.00458131
TAACGGC808.984328E-519.2509936
GATATAC1605.2750693E-1119.2484261
TACCGTA1051.7938692E-618.8556027
TATGCGA708.1297435E-418.85476512
GTCCTAC3500.018.2270811
AACGCAG24400.018.121476
TAGGACG3050.018.0313134
TCAACGC25050.017.651254
ACCGTAT750.001291503817.5985628
TATCAAC25600.017.3571852
CAACGCA25600.017.2720265
ATCAACG25800.017.1373733
TACTATA902.217337E-417.1089532
GTATATA1307.32196E-716.9216961
GTATGCG800.001989914316.4979211
CGTCGTA2950.016.4054410
Produced by FastQC (version 0.11.5)