##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4064143_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1126355 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.357809926710495 32.0 32.0 32.0 32.0 32.0 2 31.426014888734013 32.0 32.0 32.0 32.0 32.0 3 31.495287009868115 32.0 32.0 32.0 32.0 32.0 4 31.566940263060935 32.0 32.0 32.0 32.0 32.0 5 31.537762961055794 32.0 32.0 32.0 32.0 32.0 6 35.11496730604472 36.0 36.0 36.0 36.0 36.0 7 35.13796538391537 36.0 36.0 36.0 36.0 36.0 8 35.08019585299484 36.0 36.0 36.0 36.0 36.0 9 35.19229994096 36.0 36.0 36.0 36.0 36.0 10 35.05148465625846 36.0 36.0 36.0 36.0 36.0 11 35.18861726542698 36.0 36.0 36.0 36.0 36.0 12 35.099481957287004 36.0 36.0 36.0 36.0 36.0 13 35.12668208513302 36.0 36.0 36.0 36.0 36.0 14 35.06714756892809 36.0 36.0 36.0 36.0 36.0 15 35.05811045363141 36.0 36.0 36.0 36.0 36.0 16 35.06134921938465 36.0 36.0 36.0 36.0 36.0 17 35.03146787646879 36.0 36.0 36.0 36.0 36.0 18 35.02963808035655 36.0 36.0 36.0 36.0 36.0 19 35.023000741329334 36.0 36.0 36.0 36.0 36.0 20 35.02464409533406 36.0 36.0 36.0 36.0 36.0 21 35.011981124956165 36.0 36.0 36.0 36.0 36.0 22 34.99828650825006 36.0 36.0 36.0 36.0 36.0 23 34.95274402830369 36.0 36.0 36.0 36.0 36.0 24 34.91926790399119 36.0 36.0 36.0 36.0 36.0 25 34.888381549333914 36.0 36.0 36.0 32.0 36.0 26 34.844771852568684 36.0 36.0 36.0 32.0 36.0 27 34.83091032578539 36.0 36.0 36.0 32.0 36.0 28 34.81129306479751 36.0 36.0 36.0 32.0 36.0 29 34.7796786981014 36.0 36.0 36.0 32.0 36.0 30 34.76312352677442 36.0 36.0 36.0 32.0 36.0 31 34.75421248185519 36.0 36.0 36.0 32.0 36.0 32 34.7207230402493 36.0 36.0 36.0 32.0 36.0 33 34.705676274354 36.0 36.0 36.0 32.0 36.0 34 34.69573091964789 36.0 36.0 36.0 32.0 36.0 35 34.651068268885034 36.0 36.0 36.0 32.0 36.0 36 34.6278349188311 36.0 36.0 36.0 32.0 36.0 37 34.619854308810275 36.0 36.0 36.0 32.0 36.0 38 34.581789045194455 36.0 36.0 36.0 32.0 36.0 39 34.58777561248452 36.0 36.0 36.0 32.0 36.0 40 34.564678098823194 36.0 36.0 36.0 32.0 36.0 41 34.541031912674065 36.0 36.0 36.0 32.0 36.0 42 34.48594448464294 36.0 36.0 36.0 32.0 36.0 43 34.47804910529984 36.0 36.0 36.0 32.0 36.0 44 34.42058409648823 36.0 36.0 36.0 32.0 36.0 45 34.38595469456788 36.0 36.0 36.0 32.0 36.0 46 34.36462926874742 36.0 36.0 36.0 32.0 36.0 47 34.34863697502119 36.0 36.0 36.0 32.0 36.0 48 34.29806144599171 36.0 36.0 36.0 32.0 36.0 49 34.27871674560862 36.0 36.0 36.0 32.0 36.0 50 33.69996581894696 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 2.0 18 2.0 19 2.0 20 8.0 21 46.0 22 107.0 23 335.0 24 825.0 25 2047.0 26 4104.0 27 7490.0 28 12481.0 29 19024.0 30 27906.0 31 39337.0 32 56124.0 33 90255.0 34 189364.0 35 676896.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.208572103372404 18.273077749503223 12.187793560622877 26.330556586501498 2 15.931030257939591 20.648218269825783 37.04917392389038 26.37157754834425 3 17.981339742229554 24.5150046477519 29.03082609239926 28.472829517619285 4 12.361022945696517 16.35079526437047 36.13274678054432 35.15543500938869 5 14.280755179317357 36.951493978363835 33.441854477495994 15.325896364822814 6 33.85827191096227 35.99063882225304 16.845858406859655 13.305230859925032 7 30.026945323632425 30.634569030190306 20.86997438640571 18.468511259771564 8 28.03834318517979 33.0918166047264 19.309382307871235 19.56045790222257 9 27.642388304054112 14.150269146322733 18.39996661751036 39.8073759321128 10 15.976586152340941 26.666589721416763 31.68735606059261 25.669468065649685 11 37.10180182979611 21.091929276293886 22.662393295186686 19.143875598723316 12 24.947418793220244 23.506041178919933 28.816365739692618 22.73017428816721 13 29.608913747946907 19.602610201092023 25.13108713987659 25.657388911084478 14 23.737115461446265 19.61335286545035 25.093709770497625 31.555821902605764 15 25.148669740979933 27.370059361354926 22.188326251285158 25.29294464637999 16 25.813247770671232 25.72712873818749 23.612950881965432 24.846672609175847 17 24.15892339353347 25.99076311710173 25.042482203641132 24.807831285723672 18 24.935610772111406 24.811848505752636 26.14251482439342 24.11002589774254 19 25.824185092621775 24.737227605861385 25.289984063638904 24.148603237877932 20 26.076043993508225 24.252451285038017 24.89825524615838 24.77324947529538 21 26.864176924681825 24.12427698194619 24.334601435604228 24.676944657767756 22 26.036952891322702 24.262642351021395 24.731767039285195 24.968637718370708 23 24.590401173351705 24.208546929629087 25.49056861745858 25.71048327956063 24 25.20413263716195 24.63432802030628 25.14322799908732 25.01831134344445 25 25.054290792672205 24.436183681333024 25.280332261426814 25.229193264567957 26 24.772307960032034 25.210467530023028 25.542971600867265 24.47425290907767 27 25.327244945161002 24.767445886830917 24.781829304348676 25.12347986365941 28 24.682503604685024 24.600643516183794 25.510870877696412 25.205982001434773 29 24.929683005305737 24.70390507915989 25.279137160756015 25.08727475477836 30 24.869419764738055 24.81490590143645 25.382595872039804 24.933078461785694 31 25.378123654314848 24.500903412546556 24.669599612886614 25.45137332025198 32 25.066610554876355 24.76039288907356 24.49084321282616 25.682153343223924 33 24.661680437396008 24.58283835063775 25.17930359708142 25.576177614884816 34 25.463386742926343 24.52520449212351 25.267084958453452 24.744323806496695 35 25.75067209790433 24.421569637155915 25.242825332675146 24.584932932264607 36 24.81077169632908 24.88046986331168 24.979645471616866 25.32911296874237 37 25.65487838226572 24.64015427286836 24.75992527851864 24.945042066347277 38 24.907327909592883 24.731528399167882 25.117488233419135 25.243655457820104 39 25.526007845059194 24.537118423459177 24.58204372848069 25.354830003000938 40 25.842466191260865 24.556704216148646 25.133530314222398 24.467299278368092 41 24.91343490304709 24.897187300234393 25.416666666666664 24.77271113005185 42 25.844889290981072 25.08285752589603 24.90697672353864 24.165276459584263 43 25.137459104085373 24.411032241739804 25.260513080033913 25.190995574140906 44 24.884870363185506 24.896412181412522 24.70446286597552 25.51425458942645 45 24.854040520624324 25.026990074045134 24.971944528295186 25.14702487703535 46 24.996093555787574 24.60003338234145 24.912992833450527 25.490880228420448 47 25.340929672600975 24.660402083932624 25.056200125717815 24.942468117748586 48 25.66618546120259 25.10036951607777 24.457312103471605 24.77613291924804 49 25.17876326186354 25.165712256403427 24.487859013628093 25.16766546810494 50 25.077396632103422 25.4619160627199 24.387552259928388 25.073135045248286 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 11.0 1 15.0 2 19.0 3 54.0 4 89.0 5 117.0 6 145.0 7 137.0 8 129.0 9 175.0 10 221.0 11 352.0 12 483.0 13 878.5 14 1274.0 15 1821.0 16 2368.0 17 2534.5 18 2701.0 19 2595.5 20 2490.0 21 2755.5 22 3021.0 23 3605.0 24 4189.0 25 5127.0 26 6065.0 27 7745.5 28 9426.0 29 11310.5 30 13195.0 31 15102.0 32 17009.0 33 20263.5 34 23518.0 35 27682.0 36 31846.0 37 37109.5 38 42373.0 39 45894.5 40 49416.0 41 54045.0 42 58674.0 43 60219.0 44 61764.0 45 67606.0 46 73448.0 47 78255.0 48 83062.0 49 85852.5 50 88643.0 51 86999.5 52 85356.0 53 84320.5 54 83285.0 55 82472.5 56 81660.0 57 79313.0 58 76966.0 59 70231.5 60 63497.0 61 55863.5 62 48230.0 63 41875.0 64 35520.0 65 29690.0 66 23860.0 67 20531.0 68 17202.0 69 15371.5 70 13541.0 71 10532.0 72 7523.0 73 6453.5 74 5384.0 75 4224.0 76 3064.0 77 2656.5 78 2249.0 79 1868.5 80 1488.0 81 1192.5 82 897.0 83 726.0 84 555.0 85 394.0 86 233.0 87 170.0 88 107.0 89 78.0 90 49.0 91 44.0 92 39.0 93 28.0 94 17.0 95 19.5 96 22.0 97 19.0 98 16.0 99 16.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00861184972766135 2 5.326917357316299E-4 3 3.551278238210866E-4 4 0.0 5 0.0 6 8.878195595527165E-5 7 0.0 8 8.878195595527165E-5 9 0.0014205112952843464 10 0.0011541654274185315 11 0.0 12 1.775639119105433E-4 13 4.439097797763582E-4 14 4.439097797763582E-4 15 0.0029298045465239644 16 6.214736916869015E-4 17 8.878195595527165E-5 18 1.775639119105433E-4 19 0.0 20 6.214736916869015E-4 21 0.0 22 1.775639119105433E-4 23 7.990376035974448E-4 24 3.551278238210866E-4 25 0.0011541654274185315 26 0.004705443665629397 27 0.005149353445405755 28 0.003817624106076681 29 0.0023971128107923346 30 0.003196150414389779 31 0.006214736916869015 32 0.002841022590568693 33 0.004705443665629397 34 0.002485894766747606 35 0.0027522406346134213 36 0.006214736916869015 37 0.003462496282255594 38 0.006392300828779558 39 0.0036400601941661377 40 0.0016868571631501613 41 0.0031073684584345075 42 0.0021307669429265195 43 0.0017756391191054328 44 0.0014205112952843464 45 0.0013317293393290749 46 9.766015155079882E-4 47 0.002041984986971248 48 0.0015092932512396182 49 4.439097797763582E-4 50 0.001242947383373803 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1126355.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.71294952987624 #Duplication Level Percentage of deduplicated Percentage of total 1 82.33895418222669 50.81379723790547 2 10.752769530116463 13.271702466369367 3 2.9534425026058098 5.467969443081111 4 1.258801331597008 3.1073737217994855 5 0.6848355474544993 2.113161078816234 6 0.4254527396852701 1.575356607092679 7 0.2940589709333636 1.2703072502412605 8 0.2165551494908602 1.0691405608771407 9 0.1548383931029258 0.8599980544963196 >10 0.7965912292121908 8.668671670675682 >50 0.059549228886805465 2.5293077312577235 >100 0.058781074875521575 7.007625489387695 >500 0.005079843066125738 2.055343768638418 >1k 2.902767466357565E-4 0.19024491936148516 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 1.775639119105433E-4 6 0.0 0.0 0.0 0.0 1.775639119105433E-4 7 0.0 0.0 0.0 0.0 1.775639119105433E-4 8 0.0 0.0 0.0 0.0 2.6634586786581494E-4 9 0.0 0.0 0.0 0.0 2.6634586786581494E-4 10 0.0 0.0 0.0 0.0 2.6634586786581494E-4 11 0.0 0.0 0.0 0.0 2.6634586786581494E-4 12 0.0 0.0 0.0 0.0 6.214736916869015E-4 13 0.0 0.0 0.0 8.878195595527165E-5 7.102556476421732E-4 14 0.0 0.0 0.0 1.775639119105433E-4 7.990376035974449E-4 15 0.0 0.0 0.0 1.775639119105433E-4 0.0010653834714632598 16 0.0 0.0 0.0 1.775639119105433E-4 0.0010653834714632598 17 0.0 0.0 0.0 1.775639119105433E-4 0.0010653834714632598 18 0.0 0.0 0.0 1.775639119105433E-4 0.0011541654274185315 19 0.0 0.0 0.0 2.6634586786581494E-4 0.0011541654274185315 20 0.0 0.0 0.0 2.6634586786581494E-4 0.001242947383373803 21 0.0 0.0 0.0 2.6634586786581494E-4 0.0013317293393290749 22 0.0 0.0 0.0 2.6634586786581494E-4 0.0013317293393290749 23 0.0 0.0 0.0 4.4390977977635827E-4 0.0014205112952843464 24 0.0 0.0 0.0 9.766015155079882E-4 0.0014205112952843464 25 0.0 0.0 0.0 0.001242947383373803 0.0014205112952843464 26 0.0 0.0 0.0 0.0015980752071948897 0.0014205112952843464 27 0.0 0.0 0.0 0.0021307669429265195 0.0014205112952843464 28 0.0 0.0 0.0 0.003284932370345051 0.001509293251239618 29 0.0 0.0 0.0 0.005060571489450484 0.001509293251239618 30 0.0 0.0 0.0 0.008345503859795536 0.001509293251239618 31 0.0 0.0 0.0 0.018466646838696504 0.001509293251239618 32 0.0 0.0 0.0 0.03205028609985307 0.001509293251239618 33 0.0 0.0 0.0 0.04341437646212784 0.0015980752071948897 34 0.0 0.0 0.0 0.05690923376732913 0.001775639119105433 35 0.0 0.0 0.0 0.07413293322265183 0.001775639119105433 36 0.0 0.0 0.0 0.09943579066990425 0.001775639119105433 37 0.0 0.0 0.0 0.13512613696392345 0.001775639119105433 38 0.0 0.0 0.0 0.18741870902157845 0.001775639119105433 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACG 25 0.0023541814 35.19556 2 GTATCAA 1710 0.0 26.114826 1 GGTATCA 660 0.0 21.998203 1 AACGAAT 110 1.2623968E-7 20.004581 31 TAACGGC 80 8.984328E-5 19.25099 36 GATATAC 160 5.2750693E-11 19.248426 1 TACCGTA 105 1.7938692E-6 18.855602 7 TATGCGA 70 8.1297435E-4 18.854765 12 GTCCTAC 350 0.0 18.227081 1 AACGCAG 2440 0.0 18.12147 6 TAGGACG 305 0.0 18.031313 4 TCAACGC 2505 0.0 17.65125 4 ACCGTAT 75 0.0012915038 17.598562 8 TATCAAC 2560 0.0 17.357185 2 CAACGCA 2560 0.0 17.272026 5 ATCAACG 2580 0.0 17.137373 3 TACTATA 90 2.217337E-4 17.108953 2 GTATATA 130 7.32196E-7 16.921696 1 GTATGCG 80 0.0019899143 16.49792 11 CGTCGTA 295 0.0 16.40544 10 >>END_MODULE