FastQCFastQC Report
Thu 2 Feb 2017
SRR4064142_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064142_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002310
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30970.3089862417814848No Hit
TATCAACGCAGAGTACTTTTTTTTT22810.22757430335924017No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18940.18896349432810208No Hit
GTCCTAAAGTGTGTATTTCTCATTT13310.1327932475980485No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12540.12511099360477299No Hit
CTTTAGGACGTGAAATATGGCGAGG11430.11403657551057057No Hit
GTACATGGGGTGGTATCAACGCAAA10800.10775109497061788No Hit
CTGTAGGACGTGGAATATGGCAAGA10730.10705270824395646No Hit
GGTATCAACGCAGAGTACTTTTTTT10690.10665363011443565No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10290.10266284881922758No Hit
GTCCTACAGTGGACATTTCTAAATT10080.10056768863924334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCCG1550.014.099812515
CTTATAC707.292707E-613.5648693
TCCTATA802.0093757E-613.0555342
CGTCGTA1900.013.00150410
TTAAAGC957.423114E-812.9937173
GATATAC2450.012.7878191
CCGTCGT1900.012.5014479
CGCATCG853.9342685E-612.29553913
AATGCCG1405.2750693E-1112.2181418
TCGTTCT701.0874542E-412.21752919
TACCGTC2100.012.2156987
GTCCTAG1405.2750693E-1112.2065541
GTAGCAC550.003078262812.0850653
TCAAATC550.003078262812.0850653
GTCGTAG2050.012.05017411
TTTGCGC951.0358344E-612.00258717
TTGCGCG802.854743E-511.87874618
GTTTTCG1209.960786E-911.87756115
ACCGTCG2000.011.8763728
ATGCCGG1302.5975169E-911.69541219