##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064142_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1002310
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23191427801778	32.0	32.0	32.0	32.0	32.0
2	30.791570472209198	32.0	32.0	32.0	32.0	32.0
3	30.81815107102593	32.0	32.0	32.0	32.0	32.0
4	30.850186070177887	32.0	32.0	32.0	32.0	32.0
5	30.74253773782562	32.0	32.0	32.0	32.0	32.0
6	34.40773313645479	36.0	36.0	36.0	32.0	36.0
7	34.34888607317097	36.0	36.0	36.0	32.0	36.0
8	34.29112849318075	36.0	36.0	36.0	32.0	36.0
9	34.444517165348046	36.0	36.0	36.0	32.0	36.0
10	34.100387105785636	36.0	36.0	36.0	32.0	36.0
11	34.43254980993904	36.0	36.0	36.0	32.0	36.0
12	34.22207899751574	36.0	36.0	36.0	32.0	36.0
13	34.316173638894156	36.0	36.0	36.0	32.0	36.0
14	34.21116820145464	36.0	36.0	36.0	32.0	36.0
15	34.13903882032505	36.0	36.0	36.0	32.0	36.0
16	34.13782861589728	36.0	36.0	36.0	32.0	36.0
17	34.07171034909359	36.0	36.0	36.0	32.0	36.0
18	34.06621604094541	36.0	36.0	36.0	32.0	36.0
19	34.07359599325558	36.0	36.0	36.0	32.0	36.0
20	34.049969570292625	36.0	36.0	36.0	32.0	36.0
21	34.03762708144187	36.0	36.0	36.0	32.0	36.0
22	33.99559317975476	36.0	36.0	36.0	32.0	36.0
23	33.9539044806497	36.0	36.0	36.0	32.0	36.0
24	33.937270904211275	36.0	36.0	36.0	32.0	36.0
25	33.55673494228333	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	9.0
5	11.0
6	35.0
7	19.0
8	60.0
9	67.0
10	96.0
11	35.0
12	71.0
13	52.0
14	100.0
15	136.0
16	296.0
17	363.0
18	514.0
19	687.0
20	1050.0
21	1502.0
22	2360.0
23	3680.0
24	5641.0
25	8034.0
26	11724.0
27	15516.0
28	20836.0
29	27996.0
30	36956.0
31	50106.0
32	71771.0
33	100718.0
34	212233.0
35	429635.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.95709621686148	17.61515451250782	11.588562850523958	25.839186420106735
2	15.913950386077849	20.193383147469866	39.18548000067847	24.707186465773816
3	18.709854433347633	24.715800816131058	29.11742110566802	27.456923644853287
4	12.188580676187758	15.999545030959844	37.04006720770733	34.77180708514506
5	13.8254489796122	37.56352167245217	34.10977862079769	14.501250727137935
6	32.563476747643634	36.59928675345098	17.67480427628625	13.162432222619136
7	29.13660844460988	31.057407420343424	21.507998838419574	18.297985296627125
8	27.196856507805332	34.24556087521945	19.858315293384955	18.699267323590263
9	27.64797189045663	13.98449782640161	18.97863335313113	39.38889693001063
10	15.694114170595011	27.155298923623384	32.50134272542313	24.649244180358473
11	36.397940379620216	21.025357927921274	23.90645058887653	18.670251103581975
12	24.931511499279175	23.658583825532034	30.085920122041315	21.323984553147476
13	29.702725152551036	20.176971183029295	25.59862304718699	24.521680617232676
14	22.979638131029237	20.5646489839034	26.021538013015462	30.4341748720519
15	24.248132873702296	29.116687781341817	22.65659551570223	23.978583829253658
16	24.360231307132903	25.998989628718505	25.809295415789745	23.831483648358844
17	22.99755403584086	26.567463684919883	26.347826086956523	24.087156192282734
18	23.686901264720625	25.329061015331217	28.14427760348123	22.83976011646693
19	25.321710999281095	24.826064382139148	26.14466011662273	23.707564501957027
20	25.545497028374232	24.93469612730061	25.988644714979547	23.531162129345603
21	26.028936017253773	24.52512181484144	25.041636712197462	24.404305455707327
22	25.364484469531686	25.500882191709696	25.485504996090796	23.649128342667826
23	23.595340686115424	25.370093915231646	26.320829609510355	24.71373578914258
24	24.413634026151687	25.368734803512854	26.422391196896545	23.79523997343891
25	24.277447989735244	25.351353240437952	26.443731059376823	23.92746771044998
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	313.0
1	313.0
2	448.5
3	584.0
4	584.0
5	584.0
6	1549.0
7	2514.0
8	2514.0
9	2514.0
10	2551.5
11	2589.0
12	2589.0
13	2589.0
14	2867.0
15	3145.0
16	3145.0
17	3145.0
18	5383.5
19	7622.0
20	7622.0
21	7622.0
22	12728.5
23	17835.0
24	17835.0
25	17835.0
26	27261.5
27	36688.0
28	36688.0
29	36688.0
30	46751.5
31	56815.0
32	56815.0
33	56815.0
34	65643.0
35	74471.0
36	74471.0
37	74471.0
38	82202.0
39	89933.0
40	89933.0
41	89933.0
42	99951.0
43	109969.0
44	109969.0
45	109969.0
46	119900.5
47	129832.0
48	129832.0
49	129832.0
50	130037.5
51	130243.0
52	130243.0
53	130243.0
54	117697.5
55	105152.0
56	105152.0
57	105152.0
58	95562.5
59	85973.0
60	85973.0
61	85973.0
62	74858.0
63	63743.0
64	63743.0
65	63743.0
66	52354.0
67	40965.0
68	40965.0
69	40965.0
70	30940.5
71	20916.0
72	20916.0
73	20916.0
74	16097.0
75	11278.0
76	11278.0
77	11278.0
78	8872.0
79	6466.0
80	6466.0
81	6466.0
82	4476.5
83	2487.0
84	2487.0
85	2487.0
86	1917.5
87	1348.0
88	1348.0
89	1348.0
90	966.5
91	585.0
92	585.0
93	585.0
94	412.0
95	239.0
96	239.0
97	239.0
98	422.0
99	605.0
100	605.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04160389500254412
2	0.005088246151390289
3	0.0019953906476040347
4	0.0043898594247288765
5	0.009278566511358761
6	0.013169578274186628
7	0.022647683850305793
8	0.03661541838353404
9	0.05138130917580389
10	0.061458031946204264
11	0.05706817252147539
12	0.06804282108329758
13	0.06764374295377677
14	0.07303129770230767
15	0.06355319212618851
16	0.06963913360138081
17	0.06584789137093315
18	0.08221009468128622
19	0.07881793058035937
20	0.08360686813460906
21	0.07881793058035937
22	0.08250940327842683
23	0.08729834083267653
24	0.08430525486127047
25	0.08230986421366643
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1002310.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.228305147932865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78786915505027	42.96092174502591
2	13.548948856617907	14.965709637740598
3	3.9655046058431362	6.57024295311114
4	1.6268227118690206	3.5938664461075973
5	0.837301647944912	2.312137545678433
6	0.46465053237917786	1.5397116833632014
7	0.3226216478204243	1.2472492769208727
8	0.22266738198940692	0.9838033695201837
9	0.1661420353538647	0.8258168723779703
>10	0.8856061691438664	8.913424194825675
>50	0.08294376629515189	3.240786322023538
>100	0.08002934097890932	8.786249904752951
>500	0.006895952063806006	2.430535701244923
>1k	0.001996196650049107	1.6295443473069648
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3097	0.3089862417814848	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2281	0.22757430335924017	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1894	0.18896349432810208	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1331	0.1327932475980485	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1254	0.12511099360477299	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1143	0.11403657551057057	No Hit
GTACATGGGGTGGTATCAACGCAAA	1080	0.10775109497061788	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1073	0.10705270824395646	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1069	0.10665363011443565	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1029	0.10266284881922758	No Hit
GTCCTACAGTGGACATTTCTAAATT	1008	0.10056768863924334	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	9.976953238020173E-5
7	0.0	0.0	0.0	9.976953238020173E-5	9.976953238020173E-5
8	0.0	0.0	0.0	9.976953238020173E-5	9.976953238020173E-5
9	0.0	0.0	0.0	9.976953238020173E-5	9.976953238020173E-5
10	0.0	0.0	0.0	9.976953238020173E-5	9.976953238020173E-5
11	0.0	0.0	0.0	9.976953238020173E-5	9.976953238020173E-5
12	0.0	0.0	0.0	9.976953238020173E-5	9.976953238020173E-5
13	0.0	0.0	0.0	9.976953238020173E-5	9.976953238020173E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGCCG	155	0.0	14.0998125	15
CTTATAC	70	7.292707E-6	13.564869	3
TCCTATA	80	2.0093757E-6	13.055534	2
CGTCGTA	190	0.0	13.001504	10
TTAAAGC	95	7.423114E-8	12.993717	3
GATATAC	245	0.0	12.787819	1
CCGTCGT	190	0.0	12.501447	9
CGCATCG	85	3.9342685E-6	12.295539	13
AATGCCG	140	5.2750693E-11	12.21814	18
TCGTTCT	70	1.0874542E-4	12.217529	19
TACCGTC	210	0.0	12.215698	7
GTCCTAG	140	5.2750693E-11	12.206554	1
GTAGCAC	55	0.0030782628	12.085065	3
TCAAATC	55	0.0030782628	12.085065	3
GTCGTAG	205	0.0	12.050174	11
TTTGCGC	95	1.0358344E-6	12.002587	17
TTGCGCG	80	2.854743E-5	11.878746	18
GTTTTCG	120	9.960786E-9	11.877561	15
ACCGTCG	200	0.0	11.876372	8
ATGCCGG	130	2.5975169E-9	11.695412	19
>>END_MODULE