FastQCFastQC Report
Thu 2 Feb 2017
SRR4064142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002310
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTAGGA9000.022.7320462
TTAGGAC5950.022.552223
TAGGACG8500.021.738864
GTAGGAC8800.021.2477363
CTGTAGG9300.020.8170621
AGGACGT8700.020.7334215
GTATAGG654.9315806E-420.3075541
GGACGTG8750.020.1121436
GATTTCG3950.020.04949641
GCGAATT550.004483261619.99986513
GACGTGG4350.019.7220367
ACCGTCG2250.019.553478
ATCACGG5100.019.41163335
ATTTCGT4200.019.37985442
AATCACG5000.019.35986934
TGGCGAG10400.019.03833218
GCGAGAA6200.018.808220
TCACGGA4950.018.6665436
GTCCTAC8500.018.6351661
TAGGACC7350.018.5558454