##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064142_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1002310
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.304616336263233	32.0	32.0	32.0	32.0	32.0
2	31.42068521714839	32.0	32.0	32.0	32.0	32.0
3	31.491787969789787	32.0	32.0	32.0	32.0	32.0
4	31.56593070008281	32.0	32.0	32.0	32.0	32.0
5	31.517306023086668	32.0	32.0	32.0	32.0	32.0
6	35.10393690574772	36.0	36.0	36.0	36.0	36.0
7	35.126179525296564	36.0	36.0	36.0	36.0	36.0
8	35.07409184783151	36.0	36.0	36.0	36.0	36.0
9	35.17648531891331	36.0	36.0	36.0	36.0	36.0
10	35.035195697937766	36.0	36.0	36.0	36.0	36.0
11	35.183208787700416	36.0	36.0	36.0	36.0	36.0
12	35.08842972732987	36.0	36.0	36.0	36.0	36.0
13	35.127370773513185	36.0	36.0	36.0	36.0	36.0
14	35.068199459249136	36.0	36.0	36.0	36.0	36.0
15	35.04195608145184	36.0	36.0	36.0	36.0	36.0
16	35.05952749149465	36.0	36.0	36.0	36.0	36.0
17	35.02981213397053	36.0	36.0	36.0	36.0	36.0
18	35.02085083457214	36.0	36.0	36.0	36.0	36.0
19	35.01734992168092	36.0	36.0	36.0	36.0	36.0
20	35.01028723648372	36.0	36.0	36.0	36.0	36.0
21	34.996530015663815	36.0	36.0	36.0	36.0	36.0
22	34.979831589029345	36.0	36.0	36.0	36.0	36.0
23	34.934035378276185	36.0	36.0	36.0	36.0	36.0
24	34.89845357224811	36.0	36.0	36.0	32.0	36.0
25	34.88291446757989	36.0	36.0	36.0	32.0	36.0
26	34.82306871127695	36.0	36.0	36.0	32.0	36.0
27	34.81595314822759	36.0	36.0	36.0	32.0	36.0
28	34.78048009098981	36.0	36.0	36.0	32.0	36.0
29	34.75022098951422	36.0	36.0	36.0	32.0	36.0
30	34.71895621115224	36.0	36.0	36.0	32.0	36.0
31	34.71621653979308	36.0	36.0	36.0	32.0	36.0
32	34.671611577256535	36.0	36.0	36.0	32.0	36.0
33	34.648767347427444	36.0	36.0	36.0	32.0	36.0
34	34.63318135107901	36.0	36.0	36.0	32.0	36.0
35	34.5987049914697	36.0	36.0	36.0	32.0	36.0
36	34.56117867725554	36.0	36.0	36.0	32.0	36.0
37	34.52573455318215	36.0	36.0	36.0	32.0	36.0
38	34.49468627470543	36.0	36.0	36.0	32.0	36.0
39	34.455992656962415	36.0	36.0	36.0	32.0	36.0
40	34.44812383394359	36.0	36.0	36.0	32.0	36.0
41	34.426977681555606	36.0	36.0	36.0	32.0	36.0
42	34.36756891580449	36.0	36.0	36.0	32.0	36.0
43	34.345870040207124	36.0	36.0	36.0	32.0	36.0
44	34.27793397252347	36.0	36.0	36.0	32.0	36.0
45	34.24674003052948	36.0	36.0	36.0	32.0	36.0
46	34.18591753050453	36.0	36.0	36.0	32.0	36.0
47	34.15914936496693	36.0	36.0	36.0	32.0	36.0
48	34.09283754527043	36.0	36.0	36.0	32.0	36.0
49	34.06794604463689	36.0	36.0	36.0	32.0	36.0
50	33.489719747383546	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	1.0
19	2.0
20	11.0
21	36.0
22	114.0
23	345.0
24	882.0
25	2025.0
26	4075.0
27	7302.0
28	11966.0
29	18280.0
30	25817.0
31	36321.0
32	52313.0
33	83494.0
34	172526.0
35	586796.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11391465380993	18.618093360366544	12.06287217228883	26.2051198135347
2	15.131452265432706	20.971103548527743	38.57416370099661	25.323280485042947
3	17.94942657175211	25.345279131601657	29.40901222681719	27.296282069829044
4	11.81989604014726	16.704013728287656	37.17572407738125	34.300366154183834
5	13.40722930031627	37.930580359369856	34.01891630333929	14.643274036974589
6	32.742131161279765	36.901830575032825	17.29727788264685	13.058760381040557
7	28.935188649408516	31.2752853660897	21.471222946217182	18.3183030382846
8	27.074956849677246	34.552383992976225	19.722341391385896	18.65031776596063
9	27.679575374515487	13.828064591761906	18.899226275697277	39.59313375802533
10	15.551762050807245	27.349850044597513	32.4823555469531	24.61603235764214
11	36.5047739721244	20.899023256278	23.821472398758868	18.774730372838743
12	24.81041200033922	23.762627144432084	30.07707234823731	21.349888506991384
13	29.807763297835194	20.115214315787792	25.66880772937606	24.408214657000958
14	23.05281715083303	20.43715149150909	26.010394020993566	30.499637336664314
15	24.276281262035372	29.151592310931164	22.630091773143334	23.942034653890133
16	24.272775525189964	26.10804705957474	25.685819871017173	23.93335754421812
17	22.964452115612936	26.722072013648475	26.342049864812285	23.97142600592631
18	23.764903073899294	25.255659426724268	28.17012700661472	22.80931049276172
19	25.19272998646126	24.838248484249867	26.262360210274476	23.706661319014398
20	25.61536151460096	24.775191385387764	26.111821146825744	23.497625953185533
21	26.18800570681725	24.34117189292734	25.235306442118706	24.235515958136705
22	25.41938290302562	25.323404229845973	25.762292154292204	23.4949207128362
23	23.649934201533867	25.224607728401494	26.41127483405717	24.714183236007475
24	24.47593192876805	25.259427538379114	26.522418869343902	23.74222166350894
25	24.292917804064302	25.22318934278371	26.54586306530658	23.938029787845412
26	23.333516256005268	26.16366257751348	27.00108256963118	23.50173859685007
27	24.153362155754824	25.700912665668223	26.10391437485844	24.041810803718512
28	23.382661654015312	25.76596585330811	26.476656848894102	24.374715643782476
29	23.92388177790105	25.26182724839192	26.195695227794946	24.618595745912085
30	23.567202450437506	25.63341215440949	26.658485238508582	24.140900156644417
31	24.654267526092074	25.102472511025525	25.81000179601285	24.43325816686955
32	23.984778537498418	25.947026197557744	25.752567424513778	24.315627840430064
33	23.549554457371883	25.542149200657317	26.57062381954401	24.337672522426793
34	24.198203896911146	25.630935835359548	26.63025638518539	23.540603882543916
35	24.597491511827567	25.618466751207503	26.40707109910733	23.376970637857596
36	23.63308182451323	25.952237936257383	26.298170657481705	24.116509581747682
37	24.422254549227844	25.867890303694256	25.533744804232043	24.176110342845856
38	24.023907165164985	26.061404296504726	25.954540465571085	23.960148072759203
39	24.825342978298828	25.18882514342729	25.698977301072585	24.286854577201296
40	25.246861415388665	25.375467806996628	26.246479287785984	23.13119148982872
41	24.225109002564178	25.44454089217476	26.73740608817983	23.592944017081223
42	24.837122338334567	26.43706486970785	25.93511233320629	22.790700458751296
43	23.890918124739972	25.73568806726177	26.393878835829444	23.97951497216882
44	23.560205767557687	26.28694517388077	25.708772657977285	24.444076400584258
45	23.7614624894367	26.452696871467467	25.733344906818477	24.05249573227735
46	23.704926079868464	25.607054987638406	25.847103356486794	24.840915576006335
47	24.518800983350094	25.4978608942739	25.941046884727744	24.04229123764826
48	24.490015823826734	25.93780617508376	25.193407869610073	24.37877013147944
49	24.15075665666305	26.14885304258987	25.422925579464913	24.277464721282165
50	24.004709192403435	26.90395542230581	24.901251627514853	24.190083757775906
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	148.0
1	91.0
2	34.0
3	124.0
4	214.0
5	232.0
6	250.0
7	241.0
8	232.0
9	327.0
10	422.0
11	634.0
12	846.0
13	1544.5
14	2243.0
15	2814.5
16	3386.0
17	3636.5
18	3887.0
19	3988.0
20	4089.0
21	4415.5
22	4742.0
23	5394.0
24	6046.0
25	6786.0
26	7526.0
27	9849.0
28	12172.0
29	14638.5
30	17105.0
31	20073.5
32	23042.0
33	27873.0
34	32704.0
35	37399.5
36	42095.0
37	45335.0
38	48575.0
39	49363.5
40	50152.0
41	51962.0
42	53772.0
43	54213.5
44	54655.0
45	58781.5
46	62908.0
47	66259.5
48	69611.0
49	71391.5
50	73172.0
51	69952.5
52	66733.0
53	65704.0
54	64675.0
55	64291.0
56	63907.0
57	61578.0
58	59249.0
59	53686.0
60	48123.0
61	42397.5
62	36672.0
63	32053.0
64	27434.0
65	23269.5
66	19105.0
67	16529.5
68	13954.0
69	12407.5
70	10861.0
71	8502.0
72	6143.0
73	5259.5
74	4376.0
75	3431.0
76	2486.0
77	2153.5
78	1821.0
79	1495.5
80	1170.0
81	950.5
82	731.0
83	581.5
84	432.0
85	313.5
86	195.0
87	142.0
88	89.0
89	72.5
90	56.0
91	37.5
92	19.0
93	19.0
94	19.0
95	18.5
96	18.0
97	12.0
98	6.0
99	7.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008580179784697349
2	6.983867266614122E-4
3	4.988476619010087E-4
4	0.0
5	0.0
6	1.9953906476040346E-4
7	9.976953238020173E-5
8	0.0
9	0.001496542985703026
10	0.0011972343885624209
11	0.0
12	4.988476619010087E-4
13	3.990781295208069E-4
14	2.993085971406052E-4
15	0.005587093813291298
16	5.986171942812104E-4
17	0.0
18	0.0
19	9.976953238020173E-5
20	0.0012970039209426226
21	0.0
22	3.990781295208069E-4
23	6.983867266614122E-4
24	6.983867266614122E-4
25	0.0021949297123644382
26	0.006485019604713113
27	0.008081332122796341
28	0.00458939848948928
29	0.002693777374265447
30	0.0039907812952080695
31	0.008979257914218155
32	0.004090550827588271
33	0.004888707086629885
34	0.0028933164390258503
35	0.003292394568546657
36	0.00917879697897856
37	0.0051880156837704906
38	0.009976953238020173
39	0.005986171942812104
40	0.002693777374265447
41	0.0039907812952080695
42	0.0023944687771248418
43	0.0016960820504634295
44	0.0013967734533228244
45	0.001895621115223833
46	0.0011972343885624209
47	0.0021949297123644382
48	0.0023944687771248418
49	5.986171942812104E-4
50	0.001496542985703026
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1002310.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.9100554343672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94092930310833	47.29355450589683
2	13.044051780082386	15.629397304669837
3	3.8583884975373106	6.934688063243553
4	1.497141312646647	3.587752761349676
5	0.7383511974009489	2.2117330583161126
6	0.44027533356784126	1.5826151784260327
7	0.28837713396403103	1.2093683057252325
8	0.18988800023951408	0.9100960496536337
9	0.13903592695721703	0.749668508223795
>10	0.7323596929916808	7.969780904439316
>50	0.06569275781659059	2.8087263418338457
>100	0.06048279333623264	7.092276819343982
>500	0.0050262703511477335	2.0203421988781027
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.976953238020173E-5
2	0.0	0.0	0.0	0.0	9.976953238020173E-5
3	0.0	0.0	0.0	0.0	9.976953238020173E-5
4	0.0	0.0	0.0	0.0	9.976953238020173E-5
5	0.0	0.0	0.0	0.0	9.976953238020173E-5
6	0.0	0.0	0.0	0.0	9.976953238020173E-5
7	0.0	0.0	0.0	0.0	9.976953238020173E-5
8	0.0	0.0	0.0	0.0	9.976953238020173E-5
9	0.0	0.0	0.0	0.0	9.976953238020173E-5
10	0.0	0.0	0.0	0.0	9.976953238020173E-5
11	0.0	0.0	0.0	0.0	9.976953238020173E-5
12	0.0	0.0	0.0	9.976953238020173E-5	1.9953906476040346E-4
13	0.0	0.0	0.0	9.976953238020173E-5	1.9953906476040346E-4
14	0.0	0.0	0.0	1.9953906476040346E-4	1.9953906476040346E-4
15	0.0	0.0	0.0	1.9953906476040346E-4	1.9953906476040346E-4
16	0.0	0.0	0.0	3.990781295208069E-4	1.9953906476040346E-4
17	0.0	0.0	0.0	3.990781295208069E-4	1.9953906476040346E-4
18	0.0	0.0	0.0	4.988476619010087E-4	2.993085971406052E-4
19	0.0	0.0	0.0	4.988476619010087E-4	2.993085971406052E-4
20	0.0	0.0	0.0	9.976953238020174E-4	3.990781295208069E-4
21	0.0	0.0	0.0	0.0013967734533228242	3.990781295208069E-4
22	0.0	0.0	0.0	0.0016960820504634295	4.988476619010087E-4
23	0.0	0.0	0.0	0.0027935469066456484	4.988476619010087E-4
24	0.0	0.0	0.0	0.0046891680218694816	4.988476619010087E-4
25	0.0	0.0	0.0	0.005287785216150692	4.988476619010087E-4
26	0.0	0.0	0.0	0.005986171942812104	5.986171942812104E-4
27	0.0	0.0	0.0	0.006784328201853718	5.986171942812104E-4
28	0.0	0.0	0.0	0.009378336043738963	5.986171942812104E-4
29	0.0	0.0	0.0	0.014765890792269856	5.986171942812104E-4
30	0.0	0.0	0.0	0.026239387015993057	5.986171942812104E-4
31	0.0	0.0	0.0	0.049086609931059254	5.986171942812104E-4
32	0.0	0.0	0.0	0.07183406331374524	5.986171942812104E-4
33	0.0	0.0	0.0	0.09438197763167085	5.986171942812104E-4
34	0.0	0.0	0.0	0.11972343885624208	5.986171942812104E-4
35	0.0	0.0	0.0	0.14725982979317775	5.986171942812104E-4
36	0.0	0.0	0.0	0.18816533806906047	5.986171942812104E-4
37	0.0	0.0	0.0	0.250720834871447	5.986171942812104E-4
38	0.0	0.0	0.0	0.33313046861749357	5.986171942812104E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTAGGA	900	0.0	22.732046	2
TTAGGAC	595	0.0	22.55222	3
TAGGACG	850	0.0	21.73886	4
GTAGGAC	880	0.0	21.247736	3
CTGTAGG	930	0.0	20.817062	1
AGGACGT	870	0.0	20.733421	5
GTATAGG	65	4.9315806E-4	20.307554	1
GGACGTG	875	0.0	20.112143	6
GATTTCG	395	0.0	20.049496	41
GCGAATT	55	0.0044832616	19.999865	13
GACGTGG	435	0.0	19.722036	7
ACCGTCG	225	0.0	19.55347	8
ATCACGG	510	0.0	19.411633	35
ATTTCGT	420	0.0	19.379854	42
AATCACG	500	0.0	19.359869	34
TGGCGAG	1040	0.0	19.038332	18
GCGAGAA	620	0.0	18.8082	20
TCACGGA	495	0.0	18.66654	36
GTCCTAC	850	0.0	18.635166	1
TAGGACC	735	0.0	18.555845	4
>>END_MODULE