FastQCFastQC Report
Thu 2 Feb 2017
SRR4064141_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064141_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5149
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTT310.602058652165469No Hit
GTATCAACGCAGAGTACTTTTTTTT250.48553117110118466No Hit
GTACTTTTTTTTTTTTTTTTTTTTT190.36900369003690037No Hit
GGTATCAACGCAGAGTACTTTTTTT110.21363371528452124No Hit
CTTTAATATACGCTATTGGAGCTGG90.1747912215964265No Hit
CTATTGGAGCTGGAATTACCGCGGC90.1747912215964265No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG80.1553699747523791No Hit
GTCCTATTCCATTATTCCTAGCTGC80.1553699747523791No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG80.1553699747523791No Hit
GATATACACTGTTCTACAATGCCGG80.1553699747523791No Hit
ATATTAAAGTTGCTGCAGTTAAAAA80.1553699747523791No Hit
GGTTGGTTTTGATCTGATAAATGCA80.1553699747523791No Hit
CCAATGGATCCTCGTTAAAGGATTT70.1359487279083317No Hit
ACGCAGAGTACTTTTTTTTTTTTTT70.1359487279083317No Hit
GGACTCTTTCGAGGCCCTGTAATTG60.11652748106428432No Hit
CTCCCGACCCGGGGAGGTAGTGACG60.11652748106428432No Hit
ATCAACGCAGAGTACTTTTTTTTTT60.11652748106428432No Hit
GAGTACTTTTTTTTTTTTTTTTTTT60.11652748106428432No Hit
GAATAATGGAATAGGACCGCGGTTC60.11652748106428432No Hit
ATACAGGACTCTTTCGAGGCCCTGT60.11652748106428432No Hit
ACCATACTCCCCCCGGAACCCAAAG60.11652748106428432No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)