##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064141_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5149
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.048358904641677	32.0	32.0	32.0	14.0	32.0
2	27.23791027383958	32.0	21.0	32.0	14.0	32.0
3	27.41017673334628	32.0	21.0	32.0	14.0	32.0
4	27.424160031073995	32.0	21.0	32.0	14.0	32.0
5	27.46766362400466	32.0	21.0	32.0	14.0	32.0
6	30.83608467663624	36.0	21.0	36.0	14.0	36.0
7	30.751796465333076	36.0	21.0	36.0	14.0	36.0
8	30.473684210526315	36.0	21.0	36.0	14.0	36.0
9	30.85647698582249	36.0	32.0	36.0	14.0	36.0
10	29.650417556807145	36.0	21.0	36.0	14.0	36.0
11	31.267818993979414	36.0	32.0	36.0	14.0	36.0
12	30.31637211108953	36.0	21.0	36.0	14.0	36.0
13	30.625364148378328	36.0	27.0	36.0	14.0	36.0
14	30.075548650223343	36.0	21.0	36.0	14.0	36.0
15	30.153039425131094	36.0	21.0	36.0	14.0	36.0
16	30.264517382015924	36.0	21.0	36.0	14.0	36.0
17	29.932414060982715	36.0	21.0	36.0	14.0	36.0
18	30.14002718974558	36.0	21.0	36.0	14.0	36.0
19	30.20955525344727	36.0	21.0	36.0	14.0	36.0
20	29.94736842105263	36.0	21.0	36.0	14.0	36.0
21	30.10176733346281	36.0	21.0	36.0	14.0	36.0
22	29.851427461643038	36.0	21.0	36.0	14.0	36.0
23	29.83569625169936	36.0	21.0	36.0	14.0	36.0
24	29.76170130122354	36.0	21.0	36.0	14.0	36.0
25	28.998446300252475	36.0	21.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	0.0
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	2.0
15	25.0
16	39.0
17	79.0
18	107.0
19	155.0
20	145.0
21	177.0
22	163.0
23	162.0
24	145.0
25	152.0
26	160.0
27	164.0
28	188.0
29	219.0
30	247.0
31	284.0
32	356.0
33	483.0
34	746.0
35	949.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.62677287740431	18.399067417913347	12.473285408976102	26.50087429570624
2	16.197319867935523	19.498931831423576	39.98834725189357	24.31540104874733
3	17.051854729073607	24.393086036123517	31.637211108953196	26.91784812584968
4	12.410176733346281	15.303942513109343	36.72557778209361	35.56030297145077
5	14.374514374514375	36.36363636363637	34.51825951825952	14.743589743589745
6	32.09013209013209	38.17016317016317	16.666666666666664	13.073038073038074
7	28.671328671328673	30.594405594405593	23.154623154623156	17.57964257964258
8	26.734019817369344	34.48610841266757	19.6619389935885	19.117932776374587
9	26.986594132504372	13.367009908684672	20.128230036914708	39.51816592189625
10	14.688167864775597	27.044880512920148	33.78667184767826	24.480279774625995
11	36.40955896638819	20.691665047600544	23.314552166310474	19.584223819700796
12	22.79440342013214	24.640497473766032	31.228138359891176	21.336960746210647
13	29.420909444228528	19.16051301982122	25.748153905946364	25.670423630003885
14	22.153128643606685	20.365332296929655	26.89467547609794	30.58686358336572
15	24.076952973183054	28.954527788573646	23.513408472600076	23.455110765643216
16	24.67936261173727	25.359502526233968	26.000777302759424	23.960357559269337
17	22.79440342013214	27.0501360279829	25.670423630003885	24.485036921881072
18	22.79440342013214	24.93198600855033	27.691410804508354	24.582199766809172
19	24.154683249125533	25.709288767975124	26.42829382044306	23.707734162456276
20	23.47454333462884	24.640497473766032	27.535950252623397	24.349008938981733
21	26.059075009716288	24.543334628837933	25.92304702681695	23.47454333462884
22	23.824329576369998	24.018655266226194	27.08900116595414	25.068013991449668
23	23.902059852312476	24.232413525068015	28.021764477263893	23.843762145355615
24	23.78546443839876	24.057520404197437	28.33268558103381	23.824329576369998
25	23.241352506801398	24.67936261173727	27.769141080450837	24.310143801010494
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	5.0
3	8.0
4	8.0
5	8.0
6	16.5
7	25.0
8	25.0
9	25.0
10	20.0
11	15.0
12	15.0
13	15.0
14	12.0
15	9.0
16	9.0
17	9.0
18	28.5
19	48.0
20	48.0
21	48.0
22	72.5
23	97.0
24	97.0
25	97.0
26	160.0
27	223.0
28	223.0
29	223.0
30	273.0
31	323.0
32	323.0
33	323.0
34	386.5
35	450.0
36	450.0
37	450.0
38	451.5
39	453.0
40	453.0
41	453.0
42	496.5
43	540.0
44	540.0
45	540.0
46	573.5
47	607.0
48	607.0
49	607.0
50	630.5
51	654.0
52	654.0
53	654.0
54	569.5
55	485.0
56	485.0
57	485.0
58	451.0
59	417.0
60	417.0
61	417.0
62	357.5
63	298.0
64	298.0
65	298.0
66	258.5
67	219.0
68	219.0
69	219.0
70	171.5
71	124.0
72	124.0
73	124.0
74	97.5
75	71.0
76	71.0
77	71.0
78	55.5
79	40.0
80	40.0
81	40.0
82	29.5
83	19.0
84	19.0
85	19.0
86	13.0
87	7.0
88	7.0
89	7.0
90	5.5
91	4.0
92	4.0
93	4.0
94	3.0
95	2.0
96	2.0
97	2.0
98	5.5
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038842493688094774
2	0.0
3	0.0
4	0.0
5	0.019421246844047387
6	0.019421246844047387
7	0.019421246844047387
8	0.038842493688094774
9	0.038842493688094774
10	0.038842493688094774
11	0.038842493688094774
12	0.05826374053214216
13	0.05826374053214216
14	0.05826374053214216
15	0.05826374053214216
16	0.05826374053214216
17	0.05826374053214216
18	0.05826374053214216
19	0.05826374053214216
20	0.05826374053214216
21	0.05826374053214216
22	0.05826374053214216
23	0.05826374053214216
24	0.05826374053214216
25	0.05826374053214216
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5149.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	89.1435230141775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.22657952069716	83.99689260050496
2	3.6601307189542487	6.5255389395999215
3	0.8061002178649237	2.15575839968926
4	0.5664488017429194	2.019809671780928
5	0.2832244008714597	1.2623810448630801
6	0.15250544662309368	0.8156923674499902
7	0.04357298474945533	0.2718974558166634
8	0.130718954248366	0.9322198485142745
9	0.04357298474945533	0.349582443192853
>10	0.08714596949891067	1.6702272285880753
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTT	31	0.602058652165469	No Hit
GTATCAACGCAGAGTACTTTTTTTT	25	0.48553117110118466	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	19	0.36900369003690037	No Hit
GGTATCAACGCAGAGTACTTTTTTT	11	0.21363371528452124	No Hit
CTTTAATATACGCTATTGGAGCTGG	9	0.1747912215964265	No Hit
CTATTGGAGCTGGAATTACCGCGGC	9	0.1747912215964265	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	8	0.1553699747523791	No Hit
GTCCTATTCCATTATTCCTAGCTGC	8	0.1553699747523791	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	8	0.1553699747523791	No Hit
GATATACACTGTTCTACAATGCCGG	8	0.1553699747523791	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	8	0.1553699747523791	No Hit
GGTTGGTTTTGATCTGATAAATGCA	8	0.1553699747523791	No Hit
CCAATGGATCCTCGTTAAAGGATTT	7	0.1359487279083317	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	7	0.1359487279083317	No Hit
GGACTCTTTCGAGGCCCTGTAATTG	6	0.11652748106428432	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	6	0.11652748106428432	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	6	0.11652748106428432	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	6	0.11652748106428432	No Hit
GAATAATGGAATAGGACCGCGGTTC	6	0.11652748106428432	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	6	0.11652748106428432	No Hit
ACCATACTCCCCCCGGAACCCAAAG	6	0.11652748106428432	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE