##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064141_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5149
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.932025636045836	32.0	32.0	32.0	21.0	32.0
2	29.96348805593319	32.0	32.0	32.0	21.0	32.0
3	30.687900563216157	32.0	32.0	32.0	32.0	32.0
4	31.074771800349584	32.0	32.0	32.0	32.0	32.0
5	30.73373470576811	32.0	32.0	32.0	32.0	32.0
6	34.20858419110507	36.0	36.0	36.0	32.0	36.0
7	34.408817246067194	36.0	36.0	36.0	32.0	36.0
8	34.28219071664401	36.0	36.0	36.0	32.0	36.0
9	34.50359293066615	36.0	36.0	36.0	32.0	36.0
10	34.105845795300056	36.0	36.0	36.0	32.0	36.0
11	34.727131481841134	36.0	36.0	36.0	32.0	36.0
12	34.358127791804236	36.0	36.0	36.0	32.0	36.0
13	34.5503981355603	36.0	36.0	36.0	32.0	36.0
14	34.40473878422995	36.0	36.0	36.0	32.0	36.0
15	34.48727908331715	36.0	36.0	36.0	32.0	36.0
16	34.189357156729464	36.0	36.0	36.0	32.0	36.0
17	34.273645368032625	36.0	36.0	36.0	32.0	36.0
18	34.37638376383764	36.0	36.0	36.0	32.0	36.0
19	34.23305496212857	36.0	36.0	36.0	32.0	36.0
20	34.38939599922315	36.0	36.0	36.0	32.0	36.0
21	34.240629248397745	36.0	36.0	36.0	32.0	36.0
22	34.43853175373859	36.0	36.0	36.0	32.0	36.0
23	34.30957467469412	36.0	36.0	36.0	32.0	36.0
24	34.19207613128763	36.0	36.0	36.0	32.0	36.0
25	34.13614294037677	36.0	36.0	36.0	32.0	36.0
26	34.23383181200233	36.0	36.0	36.0	32.0	36.0
27	34.16605166051661	36.0	36.0	36.0	32.0	36.0
28	33.92270343756069	36.0	36.0	36.0	32.0	36.0
29	34.04661099242571	36.0	36.0	36.0	32.0	36.0
30	33.9438725966207	36.0	36.0	36.0	32.0	36.0
31	34.010293260827346	36.0	36.0	36.0	32.0	36.0
32	33.87181977082929	36.0	36.0	36.0	32.0	36.0
33	33.77723829869878	36.0	36.0	36.0	32.0	36.0
34	33.97203340454457	36.0	36.0	36.0	32.0	36.0
35	33.828510390367065	36.0	36.0	36.0	32.0	36.0
36	33.586327442221794	36.0	36.0	36.0	27.0	36.0
37	33.66770246649835	36.0	36.0	36.0	27.0	36.0
38	33.679355214604776	36.0	36.0	36.0	27.0	36.0
39	33.56515828316178	36.0	36.0	36.0	21.0	36.0
40	33.55098077296562	36.0	36.0	36.0	21.0	36.0
41	33.58477374247427	36.0	36.0	36.0	27.0	36.0
42	33.415808894931054	36.0	36.0	36.0	21.0	36.0
43	33.46125461254613	36.0	36.0	36.0	21.0	36.0
44	33.30530200038842	36.0	36.0	36.0	21.0	36.0
45	33.389784424160034	36.0	36.0	36.0	21.0	36.0
46	33.38492911244902	36.0	36.0	36.0	21.0	36.0
47	33.41619731986793	36.0	36.0	36.0	21.0	36.0
48	33.15653524956302	36.0	36.0	36.0	21.0	36.0
49	33.13750242765585	36.0	36.0	36.0	21.0	36.0
50	32.32219848514275	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	0.0
22	1.0
23	1.0
24	9.0
25	18.0
26	36.0
27	55.0
28	92.0
29	155.0
30	274.0
31	339.0
32	532.0
33	779.0
34	1169.0
35	1688.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.49184149184149	19.63869463869464	13.111888111888112	25.757575757575758
2	18.275393280248593	20.936104097883085	36.31773159836861	24.47077102349971
3	20.29520295202952	24.237716061371138	29.3843464750437	26.082734511555643
4	13.691979025053408	17.071275975917654	37.929695086424545	31.30704991260439
5	17.84812584967955	34.89998057875315	32.045057292678194	15.206836278889105
6	33.32685958438532	38.51233249174597	15.90600116527481	12.254806758593901
7	28.840551563410372	31.773159836861524	20.37288793940571	19.013400660322393
8	27.18974558166634	33.54049329966984	19.90677801514857	19.362983103515248
9	26.665371916877064	13.730821518741504	17.88696834336764	41.71683822101379
10	14.527092639347448	26.471159448436588	31.57894736842105	27.422800543794914
11	37.28879394057098	21.285686541075936	22.217906389590212	19.207613128762866
12	23.34433870654496	23.86871237133424	29.869877646144882	22.917071275975918
13	29.054185278694895	19.246455622450963	26.41289570790445	25.2864633909497
14	22.7034375606914	20.256360458341423	25.20877840357351	31.831423577393668
15	25.033987181977086	28.063701689648475	22.625752573315207	24.276558555059236
16	25.0	26.689976689976692	23.91219891219891	24.397824397824397
17	22.95591376966401	26.27694697999611	25.169935909885417	25.597203340454456
18	23.985239852398525	25.88852204311517	26.41289570790445	23.71334239658186
19	24.587298504563993	25.985628277335405	25.674888327830647	23.752184890269955
20	24.800932219848516	25.092250922509223	25.325305884637793	24.781510973004465
21	26.3934744610604	26.063313264711596	24.645562245096137	22.89765002913187
22	24.60671975140804	25.752573315206835	25.111672169353273	24.529034764031852
23	23.829869877646146	25.05340842882113	26.199261992619927	24.917459700912797
24	24.10176733346281	26.78189939794135	25.344727131481843	23.771606137114002
25	24.723247232472325	26.140998252087783	25.072829675665176	24.062924839774713
26	24.02408234608662	26.335210720528256	25.966207030491358	23.674499902893768
27	23.407148407148405	25.89355089355089	26.63170163170163	24.06759906759907
28	23.460866187609245	26.23810448630802	26.063313264711596	24.237716061371138
29	23.616236162361623	24.529034764031852	26.626529423188966	25.228199650417555
30	23.34433870654496	26.45173820159254	25.6554670809866	24.5484560108759
31	23.616236162361623	26.859584385317536	24.60671975140804	24.917459700912797
32	23.562548562548564	27.71950271950272	25.11655011655012	23.6013986013986
33	24.334822295591376	26.510001942124685	24.83977471353661	24.31540104874733
34	25.11655011655012	26.554001554001555	25.23310023310023	23.096348096348095
35	24.936880947756844	25.73315206836279	24.820353466692563	24.509613517187802
36	23.596814915517577	26.723635657409204	26.179840745775877	23.499708681297342
37	23.562548562548564	26.204351204351205	25.11655011655012	25.11655011655012
38	23.771606137114002	26.937269372693727	25.05340842882113	24.237716061371138
39	25.713730821518745	25.51951835307827	24.25713730821519	24.509613517187802
40	24.859195960380656	27.67527675276753	23.73276364342591	23.73276364342591
41	23.57739366867353	26.937269372693727	25.713730821518745	23.771606137114002
42	24.21829481452709	27.636434259079433	24.76208972616042	23.383181200233054
43	23.771606137114002	26.218683239463974	25.538939599922315	24.47077102349971
44	23.305496212856866	26.54884443581278	24.412507282967567	25.73315206836279
45	24.509613517187802	26.7624781510973	24.83977471353661	23.888133618178287
46	23.71334239658186	26.23810448630802	25.072829675665176	24.97572344144494
47	23.523698523698524	26.08780108780109	25.796425796425797	24.592074592074592
48	23.907554865022334	27.131481841134203	24.820353466692563	24.140609827150904
49	24.10176733346281	26.743056904253255	24.76208972616042	24.393086036123517
50	24.45134977665566	26.587686929500876	24.60671975140804	24.354243542435423
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	1.5
2	1.0
3	1.0
4	1.0
5	1.0
6	1.0
7	3.0
8	5.0
9	3.5
10	2.0
11	2.5
12	3.0
13	8.5
14	14.0
15	15.0
16	16.0
17	18.0
18	20.0
19	18.5
20	17.0
21	24.0
22	31.0
23	36.0
24	41.0
25	46.0
26	51.0
27	62.0
28	73.0
29	88.5
30	104.0
31	111.0
32	118.0
33	139.0
34	160.0
35	183.0
36	206.0
37	232.5
38	259.0
39	259.0
40	259.0
41	277.5
42	296.0
43	284.5
44	273.0
45	289.5
46	306.0
47	328.5
48	351.0
49	335.5
50	320.0
51	309.0
52	298.0
53	301.5
54	305.0
55	307.5
56	310.0
57	286.5
58	263.0
59	259.0
60	255.0
61	212.0
62	169.0
63	144.0
64	119.0
65	119.0
66	119.0
67	106.0
68	93.0
69	86.5
70	80.0
71	69.0
72	58.0
73	52.0
74	46.0
75	39.5
76	33.0
77	26.5
78	20.0
79	19.0
80	18.0
81	12.5
82	7.0
83	11.5
84	16.0
85	10.0
86	4.0
87	3.5
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.019421246844047387
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.019421246844047387
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.019421246844047387
28	0.0
29	0.0
30	0.0
31	0.0
32	0.019421246844047387
33	0.0
34	0.019421246844047387
35	0.0
36	0.0
37	0.019421246844047387
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.019421246844047387
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	5149.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.70751602252865
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.0	89.9592153816275
2	2.715025906735751	5.088366673140415
3	0.6010362694300518	1.6896484754321226
4	0.2901554404145078	1.0875898232666537
5	0.22797927461139897	1.0681685764226063
6	0.0621761658031088	0.349582443192853
7	0.04145077720207254	0.2718974558166634
8	0.04145077720207254	0.3107399495047582
9	0.02072538860103627	0.1747912215964265
>10	0.0	0.0
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	9	0.1747912215964265	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	8	0.1553699747523791	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	8	0.1553699747523791	No Hit
CCCCGGAACCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATG	7	0.1359487279083317	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	7	0.1359487279083317	No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG	6	0.11652748106428432	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	6	0.11652748106428432	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	6	0.11652748106428432	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0
29	0.0	0.0	0.0	0.0	0.0
30	0.0	0.0	0.0	0.0	0.0
31	0.0	0.0	0.0	0.019421246844047387	0.0
32	0.0	0.0	0.0	0.019421246844047387	0.0
33	0.0	0.0	0.0	0.038842493688094774	0.0
34	0.0	0.0	0.0	0.038842493688094774	0.0
35	0.0	0.0	0.0	0.038842493688094774	0.0
36	0.0	0.0	0.0	0.058263740532142165	0.0
37	0.0	0.0	0.0	0.07768498737618955	0.0
38	0.0	0.0	0.0	0.09710623422023694	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE