FastQCFastQC Report
Thu 2 Feb 2017
SRR4064140_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064140_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117349
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGG1470.12526736486889534No Hit
GTATCAACGCAGAGTACTTTTTTTT1180.10055475547299081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGA707.126613E-613.5714285
GGTATCA700.001463197910.8664111
GTATCAA1605.7862053E-910.1023661
TATCAAC1856.33554E-99.2432432
ATCAACG1951.4731631E-78.2785223
CATGGGG1752.3631328E-68.1393864
CAACGCA2002.1968663E-78.0755
TCAACGC2002.211982E-78.0715584
AACGCAG2156.966875E-77.5116286
AGTACAT2507.4571526E-66.4613
GAGTACA2601.3692728E-56.211538312
CGCAGAG2651.8367875E-56.09433948
ACGCAGA2702.4482008E-55.98148167
GCAGAGT2702.4482008E-55.98148169
CAGAGTA2753.2430817E-55.872727410
AGAGTAC2753.2430817E-55.872727411
ACATGGG3501.1804041E-65.69757033
GTACATG3756.9276757E-75.57809071
TACATGG3703.2616972E-65.39189152
Produced by FastQC (version 0.11.5)