FastQCFastQC Report
Thu 2 Feb 2017
SRR4064140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117349
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA301.2908118E-436.6819461
GTATCAA1650.029.3455561
GTTATAA300.00571250829.3455561
GGTAATA300.00572443329.33304813
TTATGGT300.00572443329.3330484
GATCTTT300.00572443329.33304822
GTATACA406.977058E-427.5114631
CCATATC406.9944846E-427.4997359
ATCAACG2100.023.0473963
CAACGCA2200.021.9997885
AACGCAG2200.021.9997886
TCAACGC2200.021.9997884
TTTATGG500.002566845621.9997863
AGTGAGG602.8516093E-421.9997846
TATCAAC2450.021.550812
ACCATAT550.004460185819.9998078
TAGAATA550.004460185819.99980743
CTTATAC1101.2415512E-719.99980743
CCCATAG550.004460185819.9998073
TTATGGA600.007373689718.3331552
Produced by FastQC (version 0.11.5)