##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064140_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	117349
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40451985104262	32.0	32.0	32.0	32.0	32.0
2	31.478402031546924	32.0	32.0	32.0	32.0	32.0
3	31.523438631773598	32.0	32.0	32.0	32.0	32.0
4	31.572642289239788	32.0	32.0	32.0	32.0	32.0
5	31.555394592199338	32.0	32.0	32.0	32.0	32.0
6	35.106980033915924	36.0	36.0	36.0	36.0	36.0
7	35.1353057972373	36.0	36.0	36.0	36.0	36.0
8	35.08827514508006	36.0	36.0	36.0	36.0	36.0
9	35.199089894247074	36.0	36.0	36.0	36.0	36.0
10	35.09946399202379	36.0	36.0	36.0	36.0	36.0
11	35.19309921686593	36.0	36.0	36.0	36.0	36.0
12	35.11381434865231	36.0	36.0	36.0	36.0	36.0
13	35.149613545918584	36.0	36.0	36.0	36.0	36.0
14	35.10083596792474	36.0	36.0	36.0	36.0	36.0
15	35.08235264041449	36.0	36.0	36.0	36.0	36.0
16	35.121705340480105	36.0	36.0	36.0	36.0	36.0
17	35.081790215511	36.0	36.0	36.0	36.0	36.0
18	35.08115961789193	36.0	36.0	36.0	36.0	36.0
19	35.048223674679804	36.0	36.0	36.0	36.0	36.0
20	35.08306845392803	36.0	36.0	36.0	36.0	36.0
21	35.049996165284746	36.0	36.0	36.0	36.0	36.0
22	35.01344706814715	36.0	36.0	36.0	36.0	36.0
23	34.99018312895721	36.0	36.0	36.0	36.0	36.0
24	34.99436722937563	36.0	36.0	36.0	36.0	36.0
25	34.963638377830236	36.0	36.0	36.0	36.0	36.0
26	34.91605382235895	36.0	36.0	36.0	36.0	36.0
27	34.92491627538369	36.0	36.0	36.0	36.0	36.0
28	34.926978500029826	36.0	36.0	36.0	36.0	36.0
29	34.89469871920511	36.0	36.0	36.0	36.0	36.0
30	34.9053166196559	36.0	36.0	36.0	36.0	36.0
31	34.86863117708715	36.0	36.0	36.0	32.0	36.0
32	34.86486463455164	36.0	36.0	36.0	32.0	36.0
33	34.83537993506549	36.0	36.0	36.0	32.0	36.0
34	34.822265208906764	36.0	36.0	36.0	32.0	36.0
35	34.800415853565006	36.0	36.0	36.0	32.0	36.0
36	34.79213286862266	36.0	36.0	36.0	32.0	36.0
37	34.7971776495752	36.0	36.0	36.0	32.0	36.0
38	34.77099932679443	36.0	36.0	36.0	32.0	36.0
39	34.79647035765111	36.0	36.0	36.0	32.0	36.0
40	34.73894110729533	36.0	36.0	36.0	32.0	36.0
41	34.73582220555778	36.0	36.0	36.0	32.0	36.0
42	34.680730129783804	36.0	36.0	36.0	32.0	36.0
43	34.70577508116814	36.0	36.0	36.0	32.0	36.0
44	34.661991154590154	36.0	36.0	36.0	32.0	36.0
45	34.65107499850872	36.0	36.0	36.0	32.0	36.0
46	34.60326888171182	36.0	36.0	36.0	32.0	36.0
47	34.60509250185345	36.0	36.0	36.0	32.0	36.0
48	34.56630222669132	36.0	36.0	36.0	32.0	36.0
49	34.555530937630486	36.0	36.0	36.0	32.0	36.0
50	33.97515956676239	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	5.0
22	7.0
23	15.0
24	67.0
25	167.0
26	334.0
27	659.0
28	1222.0
29	1853.0
30	2652.0
31	3751.0
32	5444.0
33	8327.0
34	17845.0
35	75000.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.87250615737308	21.706338046173908	12.94284082870998	25.47831496774304
2	14.448354907157283	22.575394762631127	39.434507324306125	23.54174300590546
3	16.48529574680222	27.166438000119303	30.45838410866916	25.889882144409317
4	11.341383394830803	18.613707828784225	40.1298690231702	29.915039753214767
5	12.079353040929194	38.977750129954245	35.816240445167836	13.126656383948735
6	29.014307748681283	40.49374089255128	18.45094547034913	12.041005888418308
7	27.205174309112135	32.24654662587666	23.60139413203351	16.9468849329777
8	25.041542748553457	35.87844804812993	21.210236133243573	17.86977307007303
9	26.307222960766268	15.572485257524626	21.28455533967345	36.83573644203565
10	13.917459884617678	29.7693205736734	34.84051845350194	21.47270108820697
11	32.429760798984226	24.50979556706917	24.73732200530043	18.323121628646174
12	21.962027473838496	27.604219927054572	30.793878037972526	19.639874561134405
13	27.605072093261068	22.671881923850428	26.885843814977672	22.83720216791083
14	21.723235817944765	21.88088522271174	28.70667836964951	27.68920058969399
15	23.25706250798926	30.12654991691167	23.90557757041203	22.710810004687033
16	22.49188318605186	29.05350706013686	26.566055100597364	21.88855465321392
17	20.88812005215213	29.09270637159243	27.261416799461436	22.757756776794007
18	21.407084849466123	29.184739537618558	28.198791638616434	21.209383974298888
19	22.51318715966902	28.360701838106845	28.198791638616434	20.9273193636077
20	23.537682789651292	28.04478985581348	27.43378668575519	20.983740668780037
21	24.37174581802998	27.510247211309853	26.79187722093925	21.326129749720916
22	22.778402699662543	28.39503016668371	27.009407914919727	21.817159218734023
23	22.409414732249378	28.341343695674404	27.570985445001195	21.678256127075024
24	22.31378196661241	28.220947771178277	27.519620959701403	21.945649302507903
25	22.258581313699423	28.043085523400485	27.98087739032621	21.71745577257388
26	22.215404543982547	27.420701879974775	28.400743126928123	21.963150449114554
27	22.128290566970335	28.3817526396973	27.56108161543509	21.928875177897275
28	22.61280838552985	27.278537645404576	28.336102944309516	21.772551024756062
29	22.069776558212464	28.3477919997273	27.80921377805805	21.773217664002182
30	21.946584401418054	28.22641123534224	27.628170166348514	22.19883419689119
31	22.92037736170648	27.67745289370297	27.558994724776507	21.843175019814044
32	21.541791655304063	28.517862012544313	27.54550722661576	22.394839105535862
33	22.299393236978457	28.126704390509953	27.924734115080447	21.649168257431143
34	22.187755658576496	27.610274065994002	28.364466866648485	21.83750340878102
35	22.399100081810744	28.040632669757294	27.569368693755113	21.990898554676846
36	22.266726890004346	27.808694318268977	27.72688148217588	22.197697309550797
37	22.43943177073125	27.657289917935696	27.62831602000903	22.274962291324023
38	22.131035775766563	27.728349610540132	28.44676245504593	21.693852158647374
39	21.8754793516609	27.881649140149644	27.578272800095444	22.664598708094015
40	22.032945026289553	27.754437693336854	28.223985274442466	21.988632005931127
41	22.32438835249303	27.58315082618218	27.48174218343886	22.61071863788593
42	22.029536332415827	27.760402907615873	27.50901173442866	22.70104902553964
43	22.02830872014248	27.158305567154382	28.221799930122966	22.591585782580168
44	21.683369124314005	27.738862187681086	28.006442376521118	22.57132631148379
45	21.227460203838156	27.83089613798275	28.15131063162559	22.7903330265535
46	21.727681767051845	27.59399393257661	28.023485700651058	22.654838599720488
47	21.50715399626748	27.630020366945896	28.163480958183847	22.699344678602777
48	21.68251695810751	28.19136244333095	27.607628591880562	22.518492006680983
49	22.227903330265537	26.53901216893343	28.464907795616455	22.76817670518458
50	21.195077887991275	26.8517571667178	29.115110611173606	22.838054334117327
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.5
2	1.0
3	6.5
4	12.0
5	10.5
6	9.0
7	12.5
8	16.0
9	19.5
10	23.0
11	43.5
12	64.0
13	99.5
14	135.0
15	149.5
16	164.0
17	177.0
18	190.0
19	226.5
20	263.0
21	351.5
22	440.0
23	622.0
24	804.0
25	1056.0
26	1308.0
27	1677.5
28	2047.0
29	2405.0
30	2763.0
31	3408.0
32	4053.0
33	4475.5
34	4898.0
35	5647.5
36	6397.0
37	7072.5
38	7748.0
39	8653.5
40	9559.0
41	10060.0
42	10561.0
43	10218.5
44	9876.0
45	9877.5
46	9879.0
47	9579.0
48	9279.0
49	8808.5
50	8338.0
51	7983.5
52	7629.0
53	6802.5
54	5976.0
55	5457.5
56	4939.0
57	4211.0
58	3483.0
59	2888.5
60	2294.0
61	1991.0
62	1688.0
63	1428.0
64	1168.0
65	895.5
66	623.0
67	430.0
68	237.0
69	185.0
70	133.0
71	120.5
72	108.0
73	90.0
74	72.0
75	83.0
76	94.0
77	66.5
78	39.0
79	30.0
80	21.0
81	13.0
82	5.0
83	4.5
84	4.0
85	2.0
86	0.0
87	0.0
88	0.0
89	0.5
90	1.0
91	3.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008521589446863629
2	0.0
3	0.0017043178893727258
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	8.521589446863629E-4
10	0.0
11	0.0
12	8.521589446863629E-4
13	8.521589446863629E-4
14	0.0
15	0.0034086357787454515
16	0.0
17	0.0
18	0.0
19	0.0
20	8.521589446863629E-4
21	0.0
22	8.521589446863629E-4
23	8.521589446863629E-4
24	0.0
25	8.521589446863629E-4
26	0.0059651126128045405
27	0.005112953668118177
28	0.0034086357787454515
29	0.0025564768340590885
30	0.0042607947234318145
31	0.006817271557490903
32	0.0042607947234318145
33	0.0042607947234318145
34	0.0042607947234318145
35	0.0042607947234318145
36	0.006817271557490903
37	0.0017043178893727258
38	0.0059651126128045405
39	0.0025564768340590885
40	0.0017043178893727258
41	0.0017043178893727258
42	0.0017043178893727258
43	0.0
44	8.521589446863629E-4
45	8.521589446863629E-4
46	8.521589446863629E-4
47	0.0017043178893727258
48	8.521589446863629E-4
49	8.521589446863629E-4
50	8.521589446863629E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	117349.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.93562791331839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.78276597718207	28.38030149383463
2	16.13764956868565	14.825861319653342
3	7.8044708283090625	10.755098040886587
4	4.361376495686856	8.013702715830556
5	2.8698636490121507	6.591449437149017
6	1.8681012893052593	5.148744343795005
7	1.2818847973286336	4.1218928154479375
8	0.8681940450793062	3.1904830889057427
9	0.673406919580744	2.784003272290348
>10	2.3430108524255635	15.885094887898491
>50	0.009275577404693442	0.3033685843083452
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	8.521589446863629E-4	0.0
28	0.0	0.0	0.0	0.0017043178893727258	0.0
29	0.0	0.0	0.0	0.005112953668118177	0.0
30	0.0	0.0	0.0	0.013634543114981806	0.0
31	0.0	0.0	0.0	0.057946808238672674	0.0
32	0.0	0.0	0.0	0.09118100708144083	0.0
33	0.0	0.0	0.0	0.1286760006476408	0.0
34	0.0	0.0	0.0	0.18236201416288167	0.0
35	0.0	0.0	0.0	0.23604802767812252	0.0
36	0.0	0.0	0.0	0.3212639221467588	0.0
37	0.0	0.0	0.0	0.45760935329657687	0.0
38	0.0	0.0	0.0	0.6459364800722631	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	30	1.2908118E-4	36.681946	1
GTATCAA	165	0.0	29.345556	1
GTTATAA	30	0.005712508	29.345556	1
GGTAATA	30	0.005724433	29.333048	13
TTATGGT	30	0.005724433	29.333048	4
GATCTTT	30	0.005724433	29.333048	22
GTATACA	40	6.977058E-4	27.511463	1
CCATATC	40	6.9944846E-4	27.499735	9
ATCAACG	210	0.0	23.047396	3
CAACGCA	220	0.0	21.999788	5
AACGCAG	220	0.0	21.999788	6
TCAACGC	220	0.0	21.999788	4
TTTATGG	50	0.0025668456	21.999786	3
AGTGAGG	60	2.8516093E-4	21.999784	6
TATCAAC	245	0.0	21.55081	2
ACCATAT	55	0.0044601858	19.999807	8
TAGAATA	55	0.0044601858	19.999807	43
CTTATAC	110	1.2415512E-7	19.999807	43
CCCATAG	55	0.0044601858	19.999807	3
TTATGGA	60	0.0073736897	18.333155	2
>>END_MODULE