FastQCFastQC Report
Thu 2 Feb 2017
SRR4064139_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4064139_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1120967
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21660.19322602717118345No Hit
TATCAACGCAGAGTACTTTTTTTTT14070.12551662983834494No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11280.10062740473180745No Hit
GATTAAGAGGGACGGCCGGGGGCAT11240.10027056996325494No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA350.00216938416.28605810
AACGAAC1101.2732926E-1114.68343914
TAACGAA1101.2732926E-1114.68278413
TAGATTA400.005283400414.2477575
CTAGACA602.5729754E-514.2464854
ACGGTAT1500.013.9342499
CGCGTAA759.663454E-713.93362710
CGGACAT1101.8553692E-1013.8160065
CGATAAC1205.0931703E-1113.45861810
TAATTCC1700.013.4084574
GCATAAT500.001502386613.2961251
CGTCAAT1450.013.10723719
TCGCGTA801.9965028E-613.063369
ACGAACG1251.0186341E-1012.92200515
CCGATAA1251.0186341E-1012.920859
CGAACGA1352.7284841E-1112.668632516
GTACCGT604.0912497E-412.6663696
GCGTAAC905.40198E-712.66636911
GTATTAA905.4195516E-712.6629771
CTTACAC751.4835052E-512.6618453
Produced by FastQC (version 0.11.5)