##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4064139_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1120967
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.238321021047007	32.0	32.0	32.0	32.0	32.0
2	30.799583752242484	32.0	32.0	32.0	32.0	32.0
3	30.814162236711695	32.0	32.0	32.0	32.0	32.0
4	30.850149023120217	32.0	32.0	32.0	32.0	32.0
5	30.731356944495243	32.0	32.0	32.0	32.0	32.0
6	34.40418406607866	36.0	36.0	36.0	32.0	36.0
7	34.33644790613818	36.0	36.0	36.0	32.0	36.0
8	34.29272940238205	36.0	36.0	36.0	32.0	36.0
9	34.46056128324919	36.0	36.0	36.0	32.0	36.0
10	34.10740548116046	36.0	36.0	36.0	32.0	36.0
11	34.42650408085162	36.0	36.0	36.0	32.0	36.0
12	34.216024200534	36.0	36.0	36.0	32.0	36.0
13	34.32389624315435	36.0	36.0	36.0	32.0	36.0
14	34.20751993591247	36.0	36.0	36.0	32.0	36.0
15	34.12656304779713	36.0	36.0	36.0	32.0	36.0
16	34.13158906551219	36.0	36.0	36.0	32.0	36.0
17	34.054221043081554	36.0	36.0	36.0	32.0	36.0
18	34.05043145783953	36.0	36.0	36.0	32.0	36.0
19	34.06724997256833	36.0	36.0	36.0	32.0	36.0
20	34.04507001544202	36.0	36.0	36.0	32.0	36.0
21	34.02231020181682	36.0	36.0	36.0	32.0	36.0
22	34.009993157693316	36.0	36.0	36.0	32.0	36.0
23	33.93882157101859	36.0	36.0	36.0	32.0	36.0
24	33.91937675239325	36.0	36.0	36.0	32.0	36.0
25	33.50325834748035	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	10.0
5	9.0
6	41.0
7	19.0
8	60.0
9	55.0
10	101.0
11	40.0
12	67.0
13	50.0
14	103.0
15	186.0
16	330.0
17	449.0
18	566.0
19	814.0
20	1250.0
21	1842.0
22	2893.0
23	4280.0
24	6489.0
25	9232.0
26	13073.0
27	17394.0
28	23283.0
29	31288.0
30	41135.0
31	56014.0
32	79022.0
33	112021.0
34	236665.0
35	482184.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51667697459252	16.999153054403536	11.35995901818568	26.12421095281826
2	16.82558743288839	19.693119956035094	37.34804539947578	26.13324721160074
3	19.223872608055668	23.62255229938891	28.266113564387354	28.887461528168075
4	12.848461274943618	15.35040609209512	35.52253133118273	36.27860130177852
5	14.90710382513661	36.30610014497602	33.347340247574444	15.439455782312924
6	34.464355017865984	35.01563552498339	16.868077209605254	13.651932247545378
7	30.26947301923813	30.10529621866922	20.672803666853152	18.952427095239496
8	28.228984472603962	32.843744422630735	19.289398536498304	19.637872568267
9	27.72209628188423	13.91705192510219	18.042946646912874	40.317905146100706
10	16.193436152484626	26.46220061784319	31.124140781131594	26.220222448540586
11	37.60568873846494	20.600969841724613	22.38588561651355	19.40745580329689
12	25.203429105983084	23.00516293966216	28.750450775333576	23.040957179021184
13	30.08812035498078	19.164838264514596	24.946843930924782	25.800197449579837
14	23.922618101836893	19.409834485147222	24.71064804483237	31.956899368183517
15	25.3602943407948	27.053003836355945	21.78680643450971	25.79989538833955
16	25.942350728448922	25.21671366481084	23.710630685637312	25.130304921102926
17	24.488119955511202	25.424223543714884	24.769745474800096	25.317911025973817
18	25.149693114607746	24.592433880147304	25.882557750251088	24.37531525499386
19	26.190984183890727	24.317189897758144	24.762561965100467	24.729263953250662
20	26.343987144005	23.996964556736007	24.481921257030624	25.17712704222837
21	27.34881968702937	23.715171550007184	23.78899969558165	25.1470090673818
22	26.477940257467818	24.015658756941097	24.344367668327173	25.162033317263916
23	24.889717009516342	23.788181663159456	24.962212284377895	26.35988904294631
24	25.378630635938777	24.501519878945324	24.783266601497115	25.336582883618785
25	25.425847230653858	24.21874442024069	24.79189729671821	25.563511052387245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	75.0
1	75.0
2	194.5
3	314.0
4	314.0
5	314.0
6	726.0
7	1138.0
8	1138.0
9	1138.0
10	1310.5
11	1483.0
12	1483.0
13	1483.0
14	1868.5
15	2254.0
16	2254.0
17	2254.0
18	3929.5
19	5605.0
20	5605.0
21	5605.0
22	9540.0
23	13475.0
24	13475.0
25	13475.0
26	21071.5
27	28668.0
28	28668.0
29	28668.0
30	37339.5
31	46011.0
32	46011.0
33	46011.0
34	56552.5
35	67094.0
36	67094.0
37	67094.0
38	77632.0
39	88170.0
40	88170.0
41	88170.0
42	101917.0
43	115664.0
44	115664.0
45	115664.0
46	130075.0
47	144486.0
48	144486.0
49	144486.0
50	148204.0
51	151922.0
52	151922.0
53	151922.0
54	143004.0
55	134086.0
56	134086.0
57	134086.0
58	123992.0
59	113898.0
60	113898.0
61	113898.0
62	101118.5
63	88339.0
64	88339.0
65	88339.0
66	72442.5
67	56546.0
68	56546.0
69	56546.0
70	42903.5
71	29261.0
72	29261.0
73	29261.0
74	22548.5
75	15836.0
76	15836.0
77	15836.0
78	12547.0
79	9258.0
80	9258.0
81	9258.0
82	6422.5
83	3587.0
84	3587.0
85	3587.0
86	2765.5
87	1944.0
88	1944.0
89	1944.0
90	1389.0
91	834.0
92	834.0
93	834.0
94	588.0
95	342.0
96	342.0
97	342.0
98	509.5
99	677.0
100	677.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04192808530491977
2	0.006512234526083284
3	0.0015165477663481618
4	0.0034791389933869596
5	0.008207199676707699
6	0.010883460440851514
7	0.019982747038940485
8	0.03273959001469268
9	0.04522880691403048
10	0.0551309717413626
11	0.0515626240558375
12	0.060215867193235836
13	0.06075111934606461
14	0.06289212795737965
15	0.0564691021234345
16	0.06333817141807029
17	0.06110795411461711
18	0.07511371878030307
19	0.06958277986773918
20	0.07734393608375625
21	0.07118853632622547
22	0.07377558839823117
23	0.07975257077148569
24	0.07243745801615926
25	0.07555976224099371
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1120967.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.90027036639541
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.70549789635713	45.92164650357276
2	11.524511801148813	13.114956746521642
3	3.2864864658753623	5.610059053914223
4	1.4113330989765622	3.2122093963523635
5	0.7981245457075813	2.270675121840895
6	0.4741213213518765	1.618657882283665
7	0.32209296184774766	1.2829023628574945
8	0.24152580862625378	1.0994307049036893
9	0.1667776054354447	0.8540721756303139
>10	0.9153167383018311	9.35765696266934
>50	0.06916704510602217	2.7488065060226385
>100	0.07669773219034152	9.18723677205811
>500	0.007087057705316647	2.8204585760793632
>1k	0.0012599213698340707	0.9012312352935805
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2166	0.19322602717118345	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1407	0.12551662983834494	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1128	0.10062740473180745	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1124	0.10027056996325494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.920869213812717E-5
2	0.0	0.0	0.0	0.0	8.920869213812717E-5
3	0.0	0.0	0.0	0.0	8.920869213812717E-5
4	0.0	0.0	0.0	0.0	8.920869213812717E-5
5	0.0	0.0	0.0	0.0	8.920869213812717E-5
6	0.0	0.0	0.0	0.0	8.920869213812717E-5
7	0.0	0.0	0.0	0.0	8.920869213812717E-5
8	0.0	0.0	0.0	0.0	8.920869213812717E-5
9	0.0	0.0	0.0	8.920869213812717E-5	8.920869213812717E-5
10	0.0	0.0	0.0	8.920869213812717E-5	8.920869213812717E-5
11	0.0	0.0	0.0	8.920869213812717E-5	8.920869213812717E-5
12	0.0	0.0	0.0	8.920869213812717E-5	2.676260764143815E-4
13	0.0	0.0	0.0	8.920869213812717E-5	3.568347685525087E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	35	0.002169384	16.286058	10
AACGAAC	110	1.2732926E-11	14.683439	14
TAACGAA	110	1.2732926E-11	14.682784	13
TAGATTA	40	0.0052834004	14.247757	5
CTAGACA	60	2.5729754E-5	14.246485	4
ACGGTAT	150	0.0	13.934249	9
CGCGTAA	75	9.663454E-7	13.933627	10
CGGACAT	110	1.8553692E-10	13.816006	5
CGATAAC	120	5.0931703E-11	13.458618	10
TAATTCC	170	0.0	13.408457	4
GCATAAT	50	0.0015023866	13.296125	1
CGTCAAT	145	0.0	13.107237	19
TCGCGTA	80	1.9965028E-6	13.06336	9
ACGAACG	125	1.0186341E-10	12.922005	15
CCGATAA	125	1.0186341E-10	12.92085	9
CGAACGA	135	2.7284841E-11	12.6686325	16
GTACCGT	60	4.0912497E-4	12.666369	6
GCGTAAC	90	5.40198E-7	12.666369	11
GTATTAA	90	5.4195516E-7	12.662977	1
CTTACAC	75	1.4835052E-5	12.661845	3
>>END_MODULE